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Spectrin Alpha 1 (SPTA1) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4380694

MBS4380694 is an antibody targeting SPTA1. Also known as Spectrin Alpha 1 (Erythrocyte Marker). Reactive in Human. Others not known.. Host: Mouse. Recombinant Mouse Monoclonal Antibody More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Spectrin Alpha 1 (SPTA1), Monoclonal Antibody
Full Product Name
Spectrin Alpha 1 (Erythrocyte Marker)
Product Gene Name
anti-SPTA1 antibody
Product Synonym Names
Alpha I spectrin; Elliptocytosis 2 (EL2); Erythrocyte alpha spectrin; Erythroid alpha-spectrin; HPP; HS3; Spectrin alpha erythrocytic 1; SPH3; SPTA1
Clonality
Monoclonal
Isotype
IgG1
Clone Number
rSPTA1/1833
Host
Mouse
Species Reactivity
Human. Others not known.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Specificity
Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. Spectrins function as membrane organizers and stabilizers, composed of non-homologous alpha and beta chains, which aggregate side-to-side in an anti-parallel fashion to form dimers, tetramers and higher polymers. Spectrin alpha I and spectrin beta I are present in erythrocytes, whereas spectrin alpha II (also designated fodrin alpha) and spectrin beta II (also designated fodrin beta) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
240kDa
3D Structure
ModBase 3D Structure for P02549
Immunogen
Recombinant fragment of human SPTA (around aa356-475) (exact sequence is proprietary)
Positive Control
Jurkat Cells. Placenta or Tonsil.
Cellular Localization
Cytoplasmic and Cell Surface
Chromosome Location
1q23.1
Applications Tested/Suitable for anti-SPTA1 antibody
Immunohistology (Formalin-Fixed)
Application Notes for anti-SPTA1 antibody
Immunohistology (Formalin-Fixed): 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Immunohistochemistry (IHC) of anti-SPTA1 antibody

Formalin-fixed, paraffin-embedded Human Placenta stained with Spectrin alpha 1 Mouse Recombinant Monoclonal Antibody (rSPTA1/1833).
Mouse Anti-SPTA1 Monoclonal Antibody – Cat# MBS4380694

SDS-Page of anti-SPTA1 antibody

SDS-PAGE Analysis Purified Spectrin alpha 1 Mouse Recombinant Monoclonal (rSPTA1/1833). Confirmation of Purity and Integrity of Antibody.
Mouse Anti-SPTA1 Monoclonal Antibody, SDS-Page – Cat# MBS4380694

Product References and Citations for anti-SPTA1 antibody

Speicher, D.W. 1986. J. Cell. Biochem. 30: 245-258.

Other Notes

Small volumes of anti-SPTA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SPTA1. It may not necessarily be applicable to this product.

NCBI GI #
115298659
NCBI GeneID
6708
NCBI Accession #
NP_003117.2
UniProt Primary Accession #
P02549
UniProt Secondary Accession #
Q15514; Q5VYL1; Q5VYL2; Q6LDY5
UniProt Related Accession #
P02549
GenBank Nucleotide #
NM_003126.3
NCBI Official Full Name
spectrin alpha chain, erythrocytic 1
NCBI Official Synonym Full Names
spectrin alpha, erythrocytic 1
NCBI Official Symbol
SPTA1
NCBI Official Synonym Symbols
EL2; HPP; HS3; SPH3; SPTA
UniProt Protein Name
Spectrin alpha chain, erythrocytic 1
UniProt Synonym Protein Names
Erythroid alpha-spectrin
Protein Family
Spectrin
UniProt Gene Name
SPTA1
UniProt Synonym Gene Names
SPTA
NCBI Protein Information
spectrin alpha chain, erythrocytic 1

NCBI Summary for SPTA1

This gene encodes a member of a family of molecular scaffold proteins that link the plasma membrane to the actin cytoskeleton and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. The encoded protein is primarily composed of 22 spectrin repeats which are involved in dimer formation. It forms a component of the erythrocyte plasma membrane. Mutations in this gene result in a variety of hereditary red blood cell disorders, including elliptocytosis-2, pyropoikilocytosis, and spherocytosis, type 3. [provided by RefSeq, Aug 2017]
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UniProt Comments for SPTA1

Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane.

Research Articles on SPTA1

1. a novel HE case with a His54Pro mutation in the SPTA1 gene was reported. The results suggested that the His54Pro mutation influenced the role of erythrocyte membrane proteins without reducing its level of expression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SPTA1 antibody

 Reference Product  PubMed Publications
 SPTB antibody  >21 publications with SPTA1 and SPTB
 ANK1 antibody  >19 publications with SPTA1 and ANK1
 ANK2 antibody  >1 publications with SPTA1 and ANK2
 SPTBN1 antibody  >1 publications with SPTA1 and SPTBN1

Pathways associated with anti-SPTA1 antibody

 Products by Pathway  Pathway Diagram
 ARMS-mediated Activation Pathway antibodies  ARMS-mediated Activation Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Asparagine N-linked Glycosylation Pathway antibodies  Asparagine N-linked Glycosylation Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 COPI-mediated Anterograde Transport Pathway antibodies  COPI-mediated Anterograde Transport Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram

Diseases associated with anti-SPTA1 antibody

 Disease Name  Pubmed Publications
 Anemia Antibodies  >30 publications with SPTA1 and Anemia
 Elliptocytosis 2 Antibodies  >7 publications with SPTA1 and Elliptocytosis 2
 Kidney Diseases Antibodies  >5 publications with SPTA1 and Kidney Diseases
 Edema Antibodies  >4 publications with SPTA1 and Edema
 Infant, Newborn, Diseases Antibodies  >3 publications with SPTA1 and Infant, Newborn, Diseases
 Congenital Abnormalities Antibodies  >3 publications with SPTA1 and Congenital Abnormalities
 Atrophy Antibodies  >2 publications with SPTA1 and Atrophy
 Inflammation Antibodies  >2 publications with SPTA1 and Inflammation
 Spherocytosis, Type 3 Antibodies  >2 publications with SPTA1 and Spherocytosis, Type 3
 Male Urogenital Diseases Antibodies  >2 publications with SPTA1 and Male Urogenital Diseases

Organs/Tissues associated with anti-SPTA1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >34 publications with SPTA1 and Blood
 Bone Antibodies  >3 publications with SPTA1 and Bone
 Bone Marrow Antibodies  >3 publications with SPTA1 and Bone Marrow
 Adipose Tissue Antibodies  >1 publications with SPTA1 and Adipose Tissue
 Heart Antibodies  >1 publications with SPTA1 and Heart
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