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TLE1 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4380705

MBS4380705 is an antibody targeting TLE1. Also known as TLE1 (Synovial Sarcoma Marker). Reactive in Human. Others not known.. Host: Rabbit. Recombinant Rabbit Monoclonal Antibody More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TLE1, Monoclonal Antibody
Full Product Name
TLE1 (Synovial Sarcoma Marker)
Product Gene Name
Product Synonym Names
Enhancer of split groucho 1 (ESG1); Enhancer of split groucho-like protein 1; GRG1; TLE1; Transducin like enhancer of split 1; Transducin-like enhancer protein 1
Clonality
Monoclonal
Isotype
IgG
Clone Number
TLE1/2946R
Host
Rabbit
Species Reactivity
Human. Others not known.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Specificity
Key players in the Notch pathway are the TLE genes, which are human homologs of the Drosophila groucho gene. Groucho is a transcriptional repressor that plays a key role in neurogenesis, segmentation and sex determination. Transducin-like enhancer protein 1 (TLE1) is a protein that is encoded by the TLE1 gene and is involved in control of hematopoiesis, neuronal, and terminal epithelial differentiation. Positive immunohistochemical nuclear staining with anti-TLE-1 has been shown to be a useful addition to an IHC panel when differentiating synovial sarcoma from other soft tissue malignancies.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
83kDa
3D Structure
ModBase 3D Structure for Q04724
Immunogen
Recombinant human TLE1 fragment (aa 175-338) (exact sequence is proprietary)
Positive Control
HeLa, HepG2 or Jurkat Cells. Endometrium Carcinoma or Sarcomas
Cellular Localization
Nuclear
Chromosome Location
9q21.32
Applications Tested/Suitable for anti-TLE1 antibody
Immunohistology (Formalin-Fixed)
Application Notes for anti-TLE1 antibody
Immunohistology (Formalin-Fixed): 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Immunohistochemistry (IHC) of anti-TLE1 antibody

Formalin-fixed, paraffin-embedded Human Endometrial Carcinoma stained with TLE1 Rabbit Recombinant Monoclonal Antibody (TLE1/2946R).
Rabbit Anti-TLE1 Monoclonal Antibody – Cat# MBS4380705

SDS-Page of anti-TLE1 antibody

SDS-PAGE Analysis Purified TLE1 Rabbit Recombinant Monoclonal Antibody (TLE1/2946R). Confirmation of Purity and Integrity of Antibody.
Rabbit Anti-TLE1 Monoclonal Antibody, SDS-Page – Cat# MBS4380705

Product References and Citations for anti-TLE1 antibody

Jagdis A, et al. Am J Surg Pathol. 2009; 33:1743-51.

Other Notes

Small volumes of anti-TLE1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TLE1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
transducin-like enhancer protein 1 isoform 1
NCBI Official Synonym Full Names
transducin like enhancer of split 1
NCBI Official Symbol
TLE1
NCBI Official Synonym Symbols
ESG; ESG1; GRG1
UniProt Protein Name
Transducin-like enhancer protein 1
UniProt Synonym Protein Names
E(Sp1) homolog; Enhancer of split groucho-like protein 1; ESG1
UniProt Gene Name
TLE1
UniProt Synonym Gene Names
ESG1
NCBI Protein Information
transducin-like enhancer protein 1

UniProt Comments for TLE1

Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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