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Thymidylate Synthase (TYMS) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4380708

MBS4380708 is an antibody targeting TYMS. Also known as Thymidylate Synthase (5-FU Resistance Marker). Reactive in Human. Others not known.. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Thymidylate Synthase (TYMS), Monoclonal Antibody
Full Product Name
Thymidylate Synthase (5-FU Resistance Marker)
Product Gene Name
Product Synonym Names
dTMP synthase; TMS; TS; TSase; TYMS protein; Tyms thymidylate synthetase
Clonality
Monoclonal
Isotype
IgG2c, kappa
Clone Number
TYMS/1884
Host
Mouse
Species Reactivity
Human. Others not known.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA at 1.0mg/ml
Specificity
It recognizes a protein of 36kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). It converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein has been reported to associate with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
36kDa
3D Structure
ModBase 3D Structure for P04818
Immunogen
Recombinant human thymidylate synthase protein fragment (around aa 60-174) (exact sequence is proprietary)
Positive Control
5-FU-resistant colon carcinoma cell lines (NCI H630R10, NCI H630R1); 5-FU-resistant breast cancer cell lines, MCF-Ad5 and MCF-Ad10. Testicular carcinomas.
Cellular Localization
Nuclear & Cytoplasmic
Chromosome Location
18p11.32
Applications Tested/Suitable for anti-TYMS antibody
Immunohistology (Formalin-Fixed)
Application Notes for anti-TYMS antibody
Immunohistology (Formalin-Fixed): 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Immunohistochemistry (IHC) of anti-TYMS antibody

Formalin-fixed, paraffin-embedded human Tonsil stained with Thymidylate Synthase Mouse Monoclonal Antibody (TYMS/1884).
Mouse Anti-TYMS Monoclonal Antibody – Cat# MBS4380708

SDS-Page of anti-TYMS antibody

SDS-PAGE Analysis Purified Thymidylate Synthase Mouse Monoclonal Antibody (TYMS/1884). Confirmation of Purity and Integrity of Antibody.
Mouse Anti-TYMS Monoclonal Antibody, SDS-Page – Cat# MBS4380708

Western Blot (WB) of anti-TYMS antibody

Western Blot Analysis of human HeLa Cell Lysate using Thymidylate Synthase Mouse Monoclonal Antibody (TYMS/1884).
Mouse Anti-TYMS Monoclonal Antibody, Western Blot (WB) – Cat# MBS4380708

Testing Data of anti-TYMS antibody

Analysis of Protein Array containing more than 19,000 full-length human proteins using Thymidylate Synthase (TYMS) Mouse Monoclonal Antibody (TYMS/1884). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Mouse Anti-TYMS Monoclonal Antibody, Testing Data – Cat# MBS4380708

Product References and Citations for anti-TYMS antibody

Johnston PG, et. al. Journal of the National Cancer Institute, 1997, 89(4):308-13.; Pestalozzi BC, et. al. Journal of Clinical Oncology, 1997, 15(5):1923-31.

Other Notes

Small volumes of anti-TYMS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TYMS. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
thymidylate synthase isoform 1
NCBI Official Synonym Full Names
thymidylate synthetase
NCBI Official Symbol
TYMS
NCBI Official Synonym Symbols
TS; TMS; HST422
UniProt Protein Name
Thymidylate synthase
Protein Family
UniProt Gene Name
TYMS
UniProt Synonym Gene Names
TS; TS; TSase
NCBI Protein Information
thymidylate synthase

NCBI Summary for TYMS

Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using, 10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occurring antisense transcript, mitochondrial enolase superfamily member 1 (GeneID:55556), vary inversely when cell-growth progresses from late-log to plateau phase. Polymorphisms in this gene may be associated with etiology of neoplasia, including breast cancer, and response to chemotherapy. [provided by RefSeq, Aug 2017]
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UniProt Comments for TYMS

Contributes to the de novo mitochondrial thymidylate biosynthesis pathway.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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