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Uroplakin 1a (UPK1A) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4380796

MBS4380796 is an antibody targeting UPK1A. Also known as Uroplakin 1A (Urothelial Differentiation Marker). Reactive in Human. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Uroplakin 1A (UPK1A), Monoclonal Antibody
Full Product Name
Uroplakin 1A (Urothelial Differentiation Marker)
Product Gene Name
Product Synonym Names
UP1A; UPIA; UPKA; TSPAN21
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
UPK1A/2925
Host
Mouse
Species Reactivity
Human
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Specificity
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is found in the asymmetrical unit membrane (AUM) where it can complex with other transmembrane 4 superfamily proteins. It may play a role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions. The protein may also play a role in tumor suppression.
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
29kDa
OMIM
611557
3D Structure
ModBase 3D Structure for O00322
Positive Control
Urinary Bladder.
Immunogen
Recombinant fragment (around aa 114-173) of human Uroplakin 1A (UPK1A) protein (exact sequence is proprietary)
Cellular Localization
Cell Surface
Hu-chromosome Location
19q13.12
Applications Tested/Suitable for anti-UPK1A antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-UPK1A antibody
ELISA: For coating, order Ab without BSA
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Immunohistochemistry (IHC) of anti-UPK1A antibody

Formalin-fixed, paraffin-embedded human Urothelial Carcinoma stained with Uroplakin 1A Mouse Monoclonal Antibody (UPK1A/2925).
Mouse Anti-UPK1A Monoclonal Antibody – Cat# MBS4380796

Immunohistochemistry (IHC) of anti-UPK1A antibody

Formalin-fixed, paraffin-embedded human Urothelial Carcinoma stained with Uroplakin 1A Mouse Monoclonal Antibody (UPK1A/2925).
Mouse Anti-UPK1A Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS4380796

SDS-Page of anti-UPK1A antibody

SDS-PAGE Analysis Purified Uroplakin 1A Mouse Monoclonal Antibody (UPK1A/2925). Confirmation of Purity and Integrity of Antibody.
Mouse Anti-UPK1A Monoclonal Antibody, SDS-Page – Cat# MBS4380796

Testing Data of anti-UPK1A antibody

Analysis of Protein Array containing more than 19,000 full-length human proteins using Uroplakin 1A Mouse Monoclonal Antibody (UPK1A/2925) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Mouse Anti-UPK1A Monoclonal Antibody, Testing Data – Cat# MBS4380796

Product References and Citations for anti-UPK1A antibody

Sun TT, et al. Adv Exp Med Biol. 1999;462:7-18; discussion 103

Other Notes

Small volumes of anti-UPK1A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for UPK1A. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
uroplakin-1a isoform 2
NCBI Official Synonym Full Names
uroplakin 1A
NCBI Official Symbol
UPK1A
NCBI Official Synonym Symbols
UP1A; UPIA; UPKA; TSPAN21
UniProt Protein Name
Uroplakin-1a
UniProt Synonym Protein Names
Tetraspanin-21; Tspan-21; Uroplakin Ia
Protein Family
UniProt Gene Name
UPK1A
UniProt Synonym Gene Names
TSPAN21; UP1a; Tspan-21; UPIa; UPKa
UniProt Entry Name
UPK1A_HUMAN
NCBI Protein Information
uroplakin-1a

NCBI Summary for UPK1A

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is found in the asymmetrical unit membrane (AUM) where it can complex with other transmembrane 4 superfamily proteins. It may play a role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions. The protein may also play a role in tumor suppression. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]
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UniProt Comments for UPK1A

UPK1A: Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions. Belongs to the tetraspanin (TM4SF) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 19q13.13

Cellular Component: endoplasmic reticulum; apical plasma membrane; integral to membrane; plasma membrane

Molecular Function: monosaccharide binding; protein homodimerization activity

Biological Process: epithelial cell differentiation; protein oligomerization
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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