Mouse Anti-BAX Monoclonal Antibody – Cat# MBS438080

Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with Bax Monoclonal Antibody (Clone SPM336).

BAX Antibody – Mouse Monoclonal

Name: BAX Antibody; Monoclonal; Mouse; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS438080
Price: 280 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS438080

MBS438080 is an antibody targeting BAX. Also known as Bax (Apoptosis Marker) Mouse Monoclonal Antibody. Reactive in Human, Monkey.Does not react with Mouse and Rat. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Bax, Monoclonal Antibody

Full Product Name

Bax (Apoptosis Marker) Mouse Monoclonal Antibody

Product Gene Name

anti-BAX antibody

Product Synonym Names

Apoptosis regulator BAX cytoplasmic isoform beta, Apoptosis regulator BAX membrane isoform alpha, Bax isoform psi, Bax protein cytoplasmic isoform delta, Bax protein cytoplasmic isoform gamma, Bax zeta, Bcl-2-like protein 4, BCL2 associated X protein, BCL2 associated X protein omega, BCL2 associated X protein transcript variant delta2, BCL2L4

Clonality

Monoclonal

Isotype

IgG1

Clone Number

SPM336

Host

Mouse

Species Reactivity

Human, Monkey.
Does not react with Mouse and Rat

Form/Format

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Specificity

Recognizes a protein of 21kDa, identified as the Bax protein. This MAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.

Preparation and Storage

Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Molecular Weight

21kDa

OMIM

600040

3D Structure

ModBase 3D Structure for Q07812

Cellular Localization

Cytoplasmic

Immunogen

A synthetic peptide, aa 3-16 (Cys-GSGEQPRGGGPTSS) of human bax protein.

Hu-Chromosome Location

19q13.33

Positive Control

Jurkat, K562, HL-60, or HeLa Cells. Reed-Sternberg cells in Hodgkin's lymphoma.

Applications Tested/Suitable for anti-BAX antibody

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin

Application Notes for anti-BAX antibody

Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Western Blot (0.5-1ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)

Product References and Citations for anti-BAX antibody

Hsu YT, et. al. Journal of Biological Chemistry, 1997, 272(21):13829-34. Hsu YT, et. al. PNAS, 1997, 94(8):3668-72. Wolter KG, et. al. Journal of Cell Biology, 1997, 139(5):1281-92

Other Notes

Small volumes of anti-BAX antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for BAX. It may not necessarily be applicable to this product.

NCBI GI #

612149787

NCBI GeneID

581

NCBI Accession #

NP_001278357.1

UniProt Primary Accession #

Q07812

UniProt Secondary Accession #

P55269; Q07814; Q07815; Q8WZ49; Q9NR76; Q9NYG7; Q9UCZ6; Q9UCZ7; Q9UQD6; A8K4W1

UniProt Related Accession #

Q07812

GenBank Nucleotide #

NM_001291428.1

NCBI Official Full Name

apoptosis regulator BAX isoform 1

NCBI Official Synonym Full Names

BCL2 associated X, apoptosis regulator

NCBI Official Symbol

BAX

NCBI Official Synonym Symbols

BCL2L4

UniProt Protein Name

Apoptosis regulator BAX

UniProt Synonym Protein Names

Bcl-2-like protein 4; Bcl2-L-4

Protein Family

Apoptosis regulator

UniProt Gene Name

BAX

UniProt Synonym Gene Names

BCL2L4; Bcl2-L-4

UniProt Entry Name

BAX_HUMAN

NCBI Protein Information

apoptosis regulator BAX

NCBI Summary for BAX

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Jul 2008]

UniProt Comments for BAX

Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.

Research Articles on BAX

1. Low expression of pro-apoptotic Bax protein in in Waldenstrom macroglobulinemia.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-BAX antibody

Reference Product	PubMed Publications
BID antibody	>1310 publications with BAX and BID
BAK1 antibody	>657 publications with BAX and BAK1
TP53 antibody	>528 publications with BAX and TP53
BCL2L11 antibody	>362 publications with BAX and BCL2L11
MCL1 antibody	>233 publications with BAX and MCL1
XRCC6 antibody	>57 publications with BAX and XRCC6
VDAC1 antibody	>42 publications with BAX and VDAC1
CLU antibody	>26 publications with BAX and CLU

Pathways associated with anti-BAX antibody

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Activation, Translocation And Oligomerization Of BAX Pathway antibodies	Activation, Translocation And Oligomerization Of BAX Pathway Diagram
AhR Pathway antibodies	AhR Pathway Diagram
Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies	Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies	Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies	Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram

Diseases associated with anti-BAX antibody

Disease Name	Pubmed Publications
Necrosis Antibodies	>2298 publications with BAX and Necrosis
Nervous System Diseases Antibodies	>2228 publications with BAX and Nervous System Diseases
Heart Diseases Antibodies	>2072 publications with BAX and Heart Diseases
Ischemia Antibodies	>1851 publications with BAX and Ischemia
Brain Diseases Antibodies	>1667 publications with BAX and Brain Diseases
Inflammation Antibodies	>1339 publications with BAX and Inflammation
Liver Diseases Antibodies	>1170 publications with BAX and Liver Diseases
Breast Neoplasms Antibodies	>1120 publications with BAX and Breast Neoplasms
Intestinal Neoplasms Antibodies	>936 publications with BAX and Intestinal Neoplasms
Liver Neoplasms Antibodies	>845 publications with BAX and Liver Neoplasms

Organs/Tissues associated with anti-BAX antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>3640 publications with BAX and Blood
Brain Antibodies	>2924 publications with BAX and Brain
Liver Antibodies	>2079 publications with BAX and Liver
Lung Antibodies	>1640 publications with BAX and Lung
Bone Antibodies	>924 publications with BAX and Bone
Nerve Antibodies	>856 publications with BAX and Nerve
Prostate Antibodies	>792 publications with BAX and Prostate
Skin Antibodies	>602 publications with BAX and Skin
Stomach Antibodies	>597 publications with BAX and Stomach
Eye Antibodies	>472 publications with BAX and Eye