Mouse Anti-KRT8 Monoclonal Antibody – Cat# MBS438081

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 8 Monoclonal Antibody (TS1).

Cytokeratin 8 (KRT8) Antibody – Mouse Monoclonal

Name: KRT8 Antibody; Monoclonal; Mouse; Human/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS438081
Price: 280 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS438081

MBS438081 is an antibody targeting KRT8. Also known as Cytokeratin 8 (KRT8) Mouse Monoclonal Antibody. Reactive in Human.Does not react with Rat and Ferret. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Cytokeratin 8 (KRT8), Monoclonal Antibody

Full Product Name

Cytokeratin 8 (KRT8) Mouse Monoclonal Antibody

Product Gene Name

anti-KRT8 antibody

Product Synonym Names

CARD2; CK8; CYK8; CYKER; Cytokeratin Endo A; DreK8; EndoA; K2C8; K8; Keratin 8; KRT8; Type-II Keratin Kb8

Clonality

Monoclonal

Isotype

IgG1, kappa

Clone Number

TS1

Host

Mouse

Species Reactivity

Human.
Does not react with Rat and Ferret

Form/Format

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Specificity

Epitope of this MAb is located between aa343-357 (ELAIKDANAKLSELE). Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Reportedly, anti-CK8 is useful for the differentiation of lobular (-ring-like, perinuclear-) from ductal (-peripheral-predominant-) carcinoma of the breast.

Preparation and Storage

Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Molecular Weight

52.5kDa

OMIM

148060

3D Structure

ModBase 3D Structure for P05787

Cellular Localization

Cytoplasmic

Immunogen

Keratin preparation from a human carcinoma

Hu-Chromosome Location

12q13.13

Positive Control

MCF-7 or A431 cells. Skin, Colon, lung or breast carcinoma

Applications Tested/Suitable for anti-KRT8 antibody

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin

Application Notes for anti-KRT8 antibody

Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1.0ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Product References and Citations for anti-KRT8 antibody

Guelstein VI et. al. Int J Cancer 42:147-53 (1988). Diversity in immunoreactivity of tumor-derived cytokeratin monoclonal antibodies. J. Histochem. Cytochem., 37: 1845-1854 (1989). Epitope Specificity of the Monoclonal Anticytokeratin Antibody TS1. Cancer Research, 59: 48-51, (1999). Epitope Specificity of 30 Monoclonal Antibodies against Cytokeratin Antigens: The ISOBM TD5-1 Workshop. Tumor Biology 19: 132-152, (1998). Immunohistochemical Profiles of 30 Monoclonal Antibodies against Cytokeratins 8, 18 and 19. Tumor Biology 19: 4-10, (1998)

Other Notes

Small volumes of anti-KRT8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for KRT8. It may not necessarily be applicable to this product.

NCBI GI #

372466577

NCBI GeneID

3856

NCBI Accession #

NP_001243222.1

UniProt Primary Accession #

P05787

UniProt Secondary Accession #

Q14099; Q14716; Q14717; Q53GJ0; Q6DHW5; Q6GMY0; Q6P4C7; Q96J60; A8K4H3; B0AZN5; F8VXB4

UniProt Related Accession #

P05787

GenBank Nucleotide #

NM_001256293.1

NCBI Official Full Name

keratin, type II cytoskeletal 8 isoform 2

NCBI Official Synonym Full Names

keratin 8

NCBI Official Symbol

KRT8

NCBI Official Synonym Symbols

K8; KO; CK8; CK-8; CYK8; K2C8; CARD2

UniProt Protein Name

Keratin, type II cytoskeletal 8

UniProt Synonym Protein Names

Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8

Protein Family

Keratin

UniProt Gene Name

KRT8

UniProt Synonym Gene Names

CYK8; CK-8; K8

UniProt Entry Name

K2C8_HUMAN

NCBI Protein Information

keratin, type II cytoskeletal 8

NCBI Summary for KRT8

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]

UniProt Comments for KRT8

K8: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Phosphorylation of keratins at specific sites affects their organization, assembly dynamics, and their interaction with signaling molecules. Phsophorylated by p38 kinase, regulating cellular keratin filament reorganization. Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Mutation of this protein is a risk factor for cryptogenic liver failure.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: cytoplasm; intermediate filament; nucleus

Molecular Function: protein binding

Disease: Cirrhosis, Familial

Research Articles on KRT8

1. The reactivity of rheumatoid arthritis (RA) serum IgG antibodies against K8 was significantly higher than healthy controls and systemic lupus erythematosus patients, confirming K8 as a novel autoantigen of RA.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-KRT8 antibody

Products by Pathway	Pathway Diagram
EGFR1 Signaling Pathway antibodies	EGFR1 Signaling Pathway Diagram
Signaling Mediated By P38-alpha And P38-beta Pathway antibodies	Signaling Mediated By P38-alpha And P38-beta Pathway Diagram

Diseases associated with anti-KRT8 antibody

Disease Name	Pubmed Publications
Liver Diseases Antibodies	>43 publications with KRT8 and Liver Diseases
Breast Neoplasms Antibodies	>18 publications with KRT8 and Breast Neoplasms
Adenocarcinoma Antibodies	>17 publications with KRT8 and Adenocarcinoma
Neoplasm Metastasis Antibodies	>14 publications with KRT8 and Neoplasm Metastasis
Disease Models, Animal Antibodies	>14 publications with KRT8 and Disease Models, Animal
Fibrosis Antibodies	>12 publications with KRT8 and Fibrosis
Liver Cirrhosis Antibodies	>10 publications with KRT8 and Liver Cirrhosis
Inflammation Antibodies	>10 publications with KRT8 and Inflammation
Liver Neoplasms Antibodies	>10 publications with KRT8 and Liver Neoplasms
Drug-Induced Liver Injury Antibodies	>9 publications with KRT8 and Drug-Induced Liver Injury

Organs/Tissues associated with anti-KRT8 antibody

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>56 publications with KRT8 and Liver
Skin Antibodies	>19 publications with KRT8 and Skin
Embryonic Tissue Antibodies	>17 publications with KRT8 and Embryonic Tissue
Bone Antibodies	>12 publications with KRT8 and Bone
Mouth Antibodies	>9 publications with KRT8 and Mouth
Kidney Antibodies	>9 publications with KRT8 and Kidney
Lung Antibodies	>9 publications with KRT8 and Lung
Prostate Antibodies	>7 publications with KRT8 and Prostate
Vascular Antibodies	>6 publications with KRT8 and Vascular
Mammary Gland Antibodies	>6 publications with KRT8 and Mammary Gland