Mouse Anti-CD1B Monoclonal Antibody – Cat# MBS438097

FCM staining of human PBMCs using CD1b MAb (RIV12).

CD1B Antibody – Mouse Monoclonal

Name: CD1B Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS438097
Price: 280 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS438097

MBS438097 is an antibody targeting CD1B. Reactive in Human.. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CD1b, Monoclonal Antibody

Full Product Name

CD1b

Product Gene Name

anti-CD1B antibody

Product Synonym Names

A1 domain; CD1A; Cortical thymocyte antigen CD1A; hTa1 thymocyte antigen; Ly38; T cell surface glycoprotein CD1a; T6/Leu6

Clonality

Monoclonal

Isotype

IgG1, kappa

Clone Number

RIV12

Host

Mouse

Species Reactivity

Human.

Form/Format

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Specificity

The mouse monoclonal antibody recognizes CD1b, a 44kDa type I glycoprotein associated with beta2-microglobulin. It is expressed on dendritic cells, Langerhans cells, thymocytes, and T acute lymphoblastic leukemia cells. The CD1 multigene family encodes five forms of the CD1 T-cell surface glycoprotein in human, designated CD1A, 1B, 1C, 1D and 1E. CD1, a type 1 membrane protein, has structural similarity to the MHC class I antigen and has been shown to present lipid antigens for recognition by T lymphocytes. Constitutive endocytosis of CD1B molecules and the differential sorting of MHC class II from lysosomes separate peptide- and lipid antigen-presenting molecules during dendritic cell maturation. CD1B is also expressed in interdigitating cells.

Preparation and Storage

Store at 2 to 8 degree C. Antibody is stable for 24 months.

Molecular Weight

44kDa

OMIM

188360

3D Structure

ModBase 3D Structure for P29016

Localization

Cell surface

Positive Control

MOLT4 cells. Human thymus.

Human Chromosomal Location

1q23.1

Immunogen

Human peripheral lymphocytes

Quantity and Buffer

0.5 ml at 200ug/ml with BSA and azide

Applications Tested/Suitable for anti-CD1B antibody

ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Functional Studies, Immunoprecipitation (IP), Immunohistology (IH)

Application Notes for anti-CD1B antibody

ELISA (For coating, order Ab without BSA); Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); Functional Studies (Order Ab without BSA & Azide); Immunoprecipitation (0.5-1ug/500ug protein lysate); Immunohistology (Frozen) (0.5-1.0ug/ml for 30 minutes at RT) Optimal dilution for a specific application should be determined.

Product References and Citations for anti-CD1B antibody

1. Knapp W. et al. (eds) Leukocyte Typing IV, p251-263, Oxford University Press, Oxford, 1989
2. Maher JK and Kronenberg M. The role of CD1 molecules in immune responses to infection. Curr Opin Immunol 1997, 9(4):456-461
3. Blumberg RS et al. Structure and function of the CD1 family of MHC-like cell surface proteins. Immunol Rev 1995, 147:5-29.
4. Salamone MC et al. Analysis of CD1 molecules on haematological malignancies of myeloid and lymphoid origin. II. Intracellular detection of CD1 antigens. Dis Markers 1990, 8(5):275-281.

Other Notes

Small volumes of anti-CD1B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD1B. It may not necessarily be applicable to this product.

NCBI GI #

4502645

NCBI GeneID

910

NCBI Accession #

NP_001755.1

UniProt Primary Accession #

P29016

UniProt Secondary Accession #

Q5TDK9; Q5TDL0; Q9UMM2

UniProt Related Accession #

P29016

GenBank Nucleotide #

NM_001764.2

NCBI Official Full Name

T-cell surface glycoprotein CD1b

NCBI Official Synonym Full Names

CD1b molecule

NCBI Official Symbol

CD1B

NCBI Official Synonym Symbols

R1; CD1; CD1A

UniProt Protein Name

T-cell surface glycoprotein CD1b

UniProt Synonym Protein Names

CD_antigen: CD1b

Protein Family

T-cell surface glycoprotein

UniProt Gene Name

CD1B

UniProt Entry Name

CD1B_HUMAN

NCBI Protein Information

T-cell surface glycoprotein CD1b; CD1B antigen, b polypeptide; cortical thymocyte antigen CD1B; differentiation antigen CD1-alpha-3

NCBI Summary for CD1B

This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail, and requires vesicular acidification to bind lipid antigens. [provided by RefSeq, Jul 2008]

UniProt Comments for CD1B

CD1B: Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23.1

Cellular Component: cell surface; lysosomal membrane; integral to membrane; plasma membrane; endosome membrane

Molecular Function: protein binding; beta-2-microglobulin binding; exogenous lipid antigen binding; endogenous lipid antigen binding

Biological Process: antigen processing and presentation, exogenous lipid antigen via MHC class Ib; immune response

Research Articles on CD1B

1. [review] Humans express both Group 1 (CD1a, CD1b and CD1c) and Group 2 (CD1d) CD1 molecules with nonredundant functions in response to the presentation of endogenous lipids.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD1B antibody

Reference Product	PubMed Publications
CD1A antibody	>150 publications with CD1B and CD1A
CD83 antibody	>6 publications with CD1B and CD83
PSAP antibody	>1 publications with CD1B and PSAP

Pathways associated with anti-CD1B antibody

Products by Pathway	Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram

Diseases associated with anti-CD1B antibody

Disease Name	Pubmed Publications
Leukemia Antibodies	>16 publications with CD1B and Leukemia
Lymphoma Antibodies	>11 publications with CD1B and Lymphoma
Necrosis Antibodies	>9 publications with CD1B and Necrosis
Dermatitis Antibodies	>8 publications with CD1B and Dermatitis
Lymphoma, Non-Hodgkin Antibodies	>5 publications with CD1B and Lymphoma, Non-Hodgkin
Neurologic Manifestations Antibodies	>4 publications with CD1B and Neurologic Manifestations
Hematologic Diseases Antibodies	>4 publications with CD1B and Hematologic Diseases
Neoplasms, Experimental Antibodies	>4 publications with CD1B and Neoplasms, Experimental
Lung Diseases, Interstitial Antibodies	>4 publications with CD1B and Lung Diseases, Interstitial
Bone Marrow Diseases Antibodies	>3 publications with CD1B and Bone Marrow Diseases

Organs/Tissues associated with anti-CD1B antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>205 publications with CD1B and Blood
Thymus Antibodies	>21 publications with CD1B and Thymus
Heart Antibodies	>5 publications with CD1B and Heart
Kidney Antibodies	>5 publications with CD1B and Kidney
Lung Antibodies	>4 publications with CD1B and Lung
Eye Antibodies	>1 publications with CD1B and Eye
Pharynx Antibodies	>1 publications with CD1B and Pharynx