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Serum Amyloid P/APCS (APCS) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4380985

MBS4380985 is an antibody targeting APCS. Also known as Serum Amyloid P/APCS. Reactive in Human. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Serum Amyloid P/APCS, Monoclonal Antibody
Full Product Name
Serum Amyloid P/APCS
Product Gene Name
Product Synonym Names
9.5S alpha 1 glycoprotein; Amyloid P component serum (APCS); Pentaxin related; Pentraxin 2 (PTX2); Serum Amyloid P (SAP); Serum amyloid P component
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
APCS/3240
Host
Mouse
Species Reactivity
Human
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Specificity
Serum amyloid P (SAP) is a glycoprotein belonging to the pentraxin family of proteins, which has a characteristic pentameric organization and calciumdependent ligand binding. Secreted by liver epithelial cells, SAP is found in serum and urine. Although the function of SAP has not been clearly established, it has been shown to interact with DNA and histones and is thought to play a role in scavenging nuclear material released from damaged circulating cells. Also designated PTX2, SAP is a precursor of the protein amyloid P component (AP), which is universally associated with the amyloid deposits in all forms of amyloidoses, including Alzheimer s disease. SAP is a decamer of ten identical, noncovalently linked subunits, each of which may be post-translationally modified by glycosylation.
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
200kDa (oligomeric); 26kDa (non-covalently bound subunit); 30kDa (glycosylated SAP subunit)
OMIM
104770
3D Structure
ModBase 3D Structure for P02743
Positive Control
Human endometrial carcinoma tissue (IHC).
Immunogen
Recombinant fragment of human APCS protein (around aa 143-223) (exact sequence is proprietary)
Cellular Localization
Secreted.
Hu-chromosome Location
1q23.2
Applications Tested/Suitable for anti-APCS antibody
Immunohistochemistry (IHC) Paraffin
Application Notes for anti-APCS antibody
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Immunohistochemistry (IHC) of anti-APCS antibody

Formalin-fixed, paraffin-embedded human Brain stained with Serum Amyloid P Mouse Monoclonal Antibody (APCS/3240).
Mouse Anti-APCS Monoclonal Antibody – Cat# MBS4380985

SDS-Page of anti-APCS antibody

SDS-PAGE Analysis Purified PSA Mouse Monoclonal Antibody (APCS/3240). Confirmation of Integrity and Purity of Antibody.
Mouse Anti-APCS Monoclonal Antibody, SDS-Page – Cat# MBS4380985

Testing Data of anti-APCS antibody

Analysis of Protein Array containing more than 19,000 full-length human proteins using Serum Amyloid P Mouse Monoclonal Antibody (APCS/3240). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.
Mouse Anti-APCS Monoclonal Antibody, Testing Data – Cat# MBS4380985

Product References and Citations for anti-APCS antibody

Castano AP et al. Serum amyloid P inhibits fibrosis through Fc gamma R-dependent monocyte-macrophage regulation in vivo. Sci Transl Med 1:5ra13 (2009).

Other Notes

Small volumes of anti-APCS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for APCS. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
serum amyloid P-component
NCBI Official Synonym Full Names
amyloid P component, serum
NCBI Official Symbol
APCS
NCBI Official Synonym Symbols
SAP; PTX2; HEL-S-92n
UniProt Protein Name
Serum amyloid P-component
UniProt Synonym Protein Names
9.5S alpha-1-glycoprotein
UniProt Gene Name
APCS
UniProt Synonym Gene Names
PTX2; SAP
UniProt Entry Name
SAMP_HUMAN
NCBI Protein Information
serum amyloid P-component

NCBI Summary for APCS

The protein encoded by this gene is a glycoprotein, belonging to the pentraxin family of proteins, which has a characteristic pentameric organization. These family members have considerable sequence homology which is thought to be the result of gene duplication. The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone. This protein is also thought to control the degradation of chromatin. It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo. [provided by RefSeq, Sep 2008]
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UniProt Comments for APCS

APCS: Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin. SAP is a precursor of amyloid component P which is found in basement membrane and associated with amyloid deposits. Belongs to the pentaxin family.

Protein type: Secreted, signal peptide; Secreted; Chaperone

Chromosomal Location of Human Ortholog: 1q21-q23

Cellular Component: extracellular matrix; extracellular space; protein complex; extracellular region; nucleus

Molecular Function: complement component C1q binding; unfolded protein binding; virion binding; calcium ion binding; carbohydrate binding

Biological Process: protein folding; negative regulation of virion penetration into host cell; acute-phase response; negative regulation of acute inflammatory response; innate immune response; chaperone-mediated protein complex assembly; negative regulation of monocyte differentiation; negative regulation of viral reproduction
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-APCS antibody

Pathways associated with anti-APCS antibody

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