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MART-1 / Melan-A / MLANA (MLANA) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS438101

MBS438101 is an antibody targeting MLANA. Also known as MART-1 / Melan-A / MLANA (Melanoma Marker) Mouse Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MART-1 / Melan-A / MLANA, Monoclonal Antibody
Full Product Name
MART-1 / Melan-A / MLANA (Melanoma Marker) Mouse Monoclonal Antibody
Product Gene Name
Product Synonym Names
Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen recognized by T-cells 1, MLAN-A, MLANA
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
SPM540
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Molecular Weight
20-22kDa (doublet)
OMIM
605513
3D Structure
ModBase 3D Structure for Q16655
Immunogen
Recombinant hMART-1 protein
Cellular Localization
Endoplasmic reticulum membrane, Golgi apparatus, Melanosome, trans-Golgi network membrane
Positive Control
SK-MEL-13 and SK-MEL-19 melanoma cell lines. Human melanoma tissues.
Applications Tested/Suitable for anti-MLANA antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes for anti-MLANA antibody
Flow Cytometry (Flow): 1-2ug/million cells
Immunofluorescence (IF): 1-3ug/ml
Immunohistochemistry (IHC): 1-2ug/ml 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degree C followed by cooling at RT for 20 minutes

*Optimal dilution for a specific application should be determined.

Immunohistochemistry (IHC) of anti-MLANA antibody

Formalin-fixed, paraffin-embedded human Melanoma stained with Melan-A Monoclonal Antibody (SPM540).
Mouse Anti-MLANA Monoclonal Antibody – Cat# MBS438101

Product References and Citations for anti-MLANA antibody

Kawakami Y, et. al. Journal of Immunological Methods, 1997, 202(1):13-25. Marincola FM, et. al. J of Immunotherapy with Emphasis on Tumor Immunol, 1996, 19(3):192-205

Other Notes

Small volumes of anti-MLANA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MLANA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
melanoma antigen recognized by T-cells 1
NCBI Official Synonym Full Names
melan-A
NCBI Official Symbol
MLANA
NCBI Official Synonym Symbols
MART1; MART-1
UniProt Protein Name
Melanoma antigen recognized by T-cells 1
UniProt Synonym Protein Names
Antigen LB39-AA; Antigen SK29-AA; Protein Melan-A
UniProt Gene Name
MLANA
UniProt Synonym Gene Names
MART1; MART-1
UniProt Entry Name
MAR1_HUMAN
NCBI Protein Information
melanoma antigen recognized by T-cells 1

UniProt Comments for MLANA

MLANA: Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 9p24.1

Cellular Component: endoplasmic reticulum membrane; Golgi apparatus; integral to plasma membrane; melanosome; trans-Golgi network

Molecular Function: protein binding
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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