• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

CD25/IL2RA (IL2RA) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4381016

MBS4381016 is an antibody targeting IL2RA. Also known as CD25/IL2RA (Activated Lymphocyte Marker). Reactive in Human. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD25/IL2RA, Monoclonal Antibody
Full Product Name
CD25/IL2RA (Activated Lymphocyte Marker)
Product Gene Name
anti-IL2RA antibody
Product Synonym Names
IL2 Receptor alpha; IL2R alpha chain; IL2R; IL2R subunit alpha IL2RA; Interleukin-2 receptor subunit alpha; Ly43; p55; p55 chain; T Cell Growth Factor Receptor; TAC antigen; TCGFR
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
IL2RA/2394
Host
Mouse
Species Reactivity
Human
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Specificity
Recognizes a protein of 55kDa, identified as CD25. It is expressed on activated T- and B-cells and activated monocytes/macrophages. With respect to lymphomas, CD25 is present on malignant cells of Hodgkin's disease, HTLV-1+ adult T-cell leukemia, cutaneous T-cell lymphoma, and hair cell leukemia. Increased levels of soluble CD25 are observed in the leukemias/lymphomas and inflammatory/ autoimmune diseases. CD25 molecule alone appears to function as a low affinity receptor and associates with CD122 (IL-2R chain, p75) and CD132 (common chain) to form the high affinity IL-2 receptor complex. CD25 antibodies detect three epitope regions, A, B and C. This MAb recognizes the epitope B, which is located at residue 3-104 of CD25 and doe not block IL-2 binding to CD25.
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
55kDa
OMIM
147730
3D Structure
ModBase 3D Structure for P01589
Positive Control
PHA-stimulated human lymphocytes. Human lymph node or tonsil.
Immunogen
Recombinant human IL2RA protein fragment (around aa 42-183) (exact sequence is proprietary)
Cellular Localization
Cell Surface and Cytoplasmic
Hu-chromosome Location
10p15-p14
Applications Tested/Suitable for anti-IL2RA antibody
ELISA (EIA)
Application Notes for anti-IL2RA antibody
ELISA: Use Ab at 2-4ug/ml for coating (Order Ab without BSA)

SDS-Page of anti-IL2RA antibody

SDS-PAGE Analysis Purified CD25 Mouse Monoclonal Antibody (IL2RA/2394). Confirmation of Purity and Integrity of Antibody.
Mouse Anti-IL2RA Monoclonal Antibody – Cat# MBS4381016

Testing Data of anti-IL2RA antibody

Analysis of Protein Array containing >19,000 full-length human proteins using CD25 Mouse Monoclonal Antibody (IL2RA/2394) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Mouse Anti-IL2RA Monoclonal Antibody, Testing Data – Cat# MBS4381016

Product References and Citations for anti-IL2RA antibody

T.A. Waldmann, et al, (1989) Annu Rev Biochem 58: 875-911.

Other Notes

Small volumes of anti-IL2RA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for IL2RA. It may not necessarily be applicable to this product.

NCBI GI #
4557667
NCBI GeneID
3559
NCBI Accession #
NP_000408.1
UniProt Primary Accession #
P01589
UniProt Related Accession #
P01589
GenBank Nucleotide #
NP_000408.1
NCBI Official Full Name
interleukin-2 receptor subunit alpha isoform 1
NCBI Official Synonym Full Names
interleukin 2 receptor subunit alpha
NCBI Official Symbol
IL2RA
NCBI Official Synonym Symbols
p55; CD25; IL2R; IMD41; TCGFR; IDDM10
UniProt Protein Name
Interleukin-2 receptor subunit alpha
UniProt Synonym Protein Names
TAC antigen; p55
Protein Family
Interleukin-2 receptor
UniProt Gene Name
IL2RA
UniProt Synonym Gene Names
IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA
UniProt Entry Name
IL2RA_HUMAN
NCBI Protein Information
interleukin-2 receptor subunit alpha

NCBI Summary for IL2RA

The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009]
Show more

UniProt Comments for IL2RA

IL2RA: Receptor for interleukin-2. Non-covalent dimer of an alpha and a beta subunit. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 10p15-p14

Cellular Component: integral to membrane; plasma membrane; external side of plasma membrane

Molecular Function: interleukin-2 receptor activity; drug binding; interleukin-2 binding

Biological Process: Notch signaling pathway; regulation of T cell homeostatic proliferation; apoptosis; negative regulation of immune response; negative regulation of defense response to virus; positive regulation of activated T cell proliferation; cell proliferation; negative regulation of T cell proliferation; inflammatory response to antigenic stimulus; cell surface receptor linked signal transduction; positive regulation of T cell differentiation; negative regulation of inflammatory response; immune response; inflammatory response; activated T cell apoptosis

Disease: Interleukin 2 Receptor, Alpha, Deficiency Of; Diabetes Mellitus, Insulin-dependent, 10
Show more

Research Articles on IL2RA

1. il2Ra internalizes via both clathrin-dependent and clathrin-independent endocytosis

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-IL2RA antibody

 Reference Product  PubMed Publications
 CD4 antibody  >2381 publications with IL2RA and CD4
 IL2RB antibody  >767 publications with IL2RA and IL2RB
 IL2 antibody  >94 publications with IL2RA and IL2
 STAT3 antibody  >28 publications with IL2RA and STAT3
 ICAM1 antibody  >24 publications with IL2RA and ICAM1
 STAT5B antibody  >10 publications with IL2RA and STAT5B
 NFKB1 antibody  >1 publications with IL2RA and NFKB1

Pathways associated with anti-IL2RA antibody

 Products by Pathway  Pathway Diagram
 Arf6 Trafficking Events Pathway antibodies  Arf6 Trafficking Events Pathway Diagram
 Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies  Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
 Calcium Signaling In The CD4+ TCR Pathway antibodies  Calcium Signaling In The CD4+ TCR Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Downstream Signaling In Naive CD8+ T Cells Pathway antibodies  Downstream Signaling In Naive CD8+ T Cells Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram

Diseases associated with anti-IL2RA antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >445 publications with IL2RA and Inflammation
 Necrosis Antibodies  >304 publications with IL2RA and Necrosis
 Lung Diseases Antibodies  >163 publications with IL2RA and Lung Diseases
 Liver Diseases Antibodies  >138 publications with IL2RA and Liver Diseases
 Diabetes Mellitus, Type 1 Antibodies  >132 publications with IL2RA and Diabetes Mellitus, Type 1
 Multiple Sclerosis Antibodies  >112 publications with IL2RA and Multiple Sclerosis
 Arthritis, Rheumatoid Antibodies  >108 publications with IL2RA and Arthritis, Rheumatoid
 Vascular Diseases Antibodies  >89 publications with IL2RA and Vascular Diseases
 HIV Infections Antibodies  >74 publications with IL2RA and HIV Infections
 Kidney Diseases Antibodies  >73 publications with IL2RA and Kidney Diseases

Organs/Tissues associated with anti-IL2RA antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >3210 publications with IL2RA and Blood
 Spleen Antibodies  >353 publications with IL2RA and Spleen
 Lymph Node Antibodies  >271 publications with IL2RA and Lymph Node
 Bone Antibodies  >214 publications with IL2RA and Bone
 Bone Marrow Antibodies  >201 publications with IL2RA and Bone Marrow
 Thymus Antibodies  >195 publications with IL2RA and Thymus
 Lung Antibodies  >188 publications with IL2RA and Lung
 Mixed Antibodies  >149 publications with IL2RA and Mixed
 Brain Antibodies  >97 publications with IL2RA and Brain
 Uterus Antibodies  >26 publications with IL2RA and Uterus
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000