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Cytokeratin 10 (KRT10) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS438105

MBS438105 is an antibody targeting KRT10. Also known as Cytokeratin 10 (KRT10) (Suprabasal Epithelial Marker) Mouse Monoclonal Antibody. Reactive in Human, Mouse. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Cytokeratin 10 (KRT10), Monoclonal Antibody
Full Product Name
Cytokeratin 10 (KRT10) (Suprabasal Epithelial Marker) Mouse Monoclonal Antibody
Product Gene Name
Product Synonym Names
BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
LH2
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide.
Specificity
This MAb recognizes a protein of 56.5kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.
Preparation and Storage
Antibody with azide - store at 2 to 8°C. Non-hazardous. No MSDS required.
Molecular Weight
56.5kDa
OMIM
113800
3D Structure
ModBase 3D Structure for P13645
Cellular Localization
Cytoplasmic
Immunogen
Skin extract of a human Psoriasis patient
Hu-Chromosome Location
17q21.2
Positive Control
A431, HeLa, MCF7 cells. Esophagus or tonsil.
Applications Tested/Suitable for anti-KRT10 antibody
Western Blot (WB), Immunohistochemistry (IHC) Formalin
Application Notes for anti-KRT10 antibody
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunohistochemistry (IHC) of anti-KRT10 antibody

Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with Cytokeratin 10 Monoclonal Antibody (LH2).
Mouse Anti-KRT10 Monoclonal Antibody – Cat# MBS438105

Western Blot (WB) of anti-KRT10 antibody

Western Blot Analysis of human Thymus tissue lysate using Cytokeratin 10 Mouse Monoclonal Antibody (LH2).
Mouse Anti-KRT10 Monoclonal Antibody, Western Blot (WB) – Cat# MBS438105

SDS-PAGE of anti-KRT10 antibody

SDS-PAGE Analysis Purified Cytokeratin 10 Mouse Monoclonal Antibody (LH2). Confirmation of Integrity and Purity of Antibody.
Mouse Anti-KRT10 Monoclonal Antibody, SDS-PAGE – Cat# MBS438105

Product References and Citations for anti-KRT10 antibody

Barrott JJ et al. Proc Natl Acad Sci U S A 108:12752-7 (2011) Reichelt J et al. J Cell Sci 110 (Pt 18):2175-86 (1997

Other Notes

Small volumes of anti-KRT10 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for KRT10. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
keratin, type I cytoskeletal 10
NCBI Official Synonym Full Names
keratin 10
NCBI Official Symbol
KRT10
NCBI Official Synonym Symbols
BIE; EHK; K10; KPP; BCIE; CK10
UniProt Protein Name
Keratin, type I cytoskeletal 10
UniProt Synonym Protein Names
Cytokeratin-10; CK-10; Keratin-10; K10
Protein Family
UniProt Gene Name
KRT10
UniProt Synonym Gene Names
KPP; CK-10; K10
UniProt Entry Name
K1C10_HUMAN
NCBI Protein Information
keratin, type I cytoskeletal 10

NCBI Summary for KRT10

This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. [provided by RefSeq, Jul 2008]
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UniProt Comments for KRT10

K10: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K1.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: cytoplasm; extracellular space; membrane; nucleus

Biological Process: keratinocyte differentiation

Disease: Epidermolytic Hyperkeratosis; Erythroderma, Ichthyosiform, Congenital Reticular; Ichthyosis, Cyclic, With Epidermolytic Hyperkeratosis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-KRT10 antibody

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