• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

TAG-72 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS438112

MBS438112 is an antibody targeting TAG-72. Also known as TAG-72 / CA72.4 (Tumor-Associated Glycoprotein) Mouse Monoclonal Antibody. Reactive in Human, Cow, Dog, Hamster, Rat. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TAG-72 / CA72.4 (Tumor-Associated Glycoprotein), Monoclonal Antibody
Full Product Name
TAG-72 / CA72.4 (Tumor-Associated Glycoprotein) Mouse Monoclonal Antibody
Product Gene Name
anti-TAG-72 antibody
Product Synonym Gene Name
CA72.4
Product Synonym Names
CA72.4, Tumor associated glycoprotein 72
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
B72.3
Host
Mouse
Species Reactivity
Human, Cow, Dog, Hamster, Rat
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
Recognizes an oncofetal antigen of 220kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This MAb defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80% sensitivity and 93% specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the apocrine carcinoma.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
220kDa
Cellular Localization
Cytoplasmic and Cell Surface
Immunogen
Membrane-enriched fraction of a human breast carcinoma liver metastasis
Positive Control
Jurkat cells, Breast or lung carcinoma.
Applications Tested/Suitable for anti-TAG-72 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
Application Notes for anti-TAG-72 antibody
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Immunohistochemistry (IHC) of anti-TAG-72 antibody

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with TAG-72 Monoclonal Antibody (B72.3).
Mouse Anti-TAG-72 Monoclonal Antibody – Cat# MBS438112

Immunohistochemistry (IHC) of anti-TAG-72 antibody

Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with TAG-72 Monoclonal Antibody (B72.3).
Mouse Anti-TAG-72 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS438112

Immunohistochemistry (IHC) of anti-TAG-72 antibody

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with TAG-72 Monoclonal Antibody (B72.3).
Mouse Anti-TAG-72 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS438112

Product References and Citations for anti-TAG-72 antibody

Lottich SC et. al. Breast Cancer Research and Treatment, 1985, 6(1):49-56. Thor A et. al. Cancer Research, 1986, 46(6):3118-24

Other Notes

Small volumes of anti-TAG-72 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TAG-72. It may not necessarily be applicable to this product.

NCBI GeneID
182875

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-TAG-72 antibody

 Products by Pathway  Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 RNA Pol II CTD Phosphorylation And Interaction With CE Pathway antibodies  RNA Pol II CTD Phosphorylation And Interaction With CE Pathway Diagram
 RNA Polymerase II Transcription Pathway antibodies  RNA Polymerase II Transcription Pathway Diagram
 MRNA Capping Pathway antibodies  MRNA Capping Pathway Diagram
 MRNA Processing Pathway antibodies  MRNA Processing Pathway Diagram
 MRNA Surveillance Pathway antibodies  MRNA Surveillance Pathway Diagram
 MRNA Surveillance Pathway antibodies  MRNA Surveillance Pathway Diagram
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000