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CD31/PECAM-1 (PECAM1) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4381158

MBS4381158 is an antibody targeting PECAM1. Also known as CD31/PECAM-1 (Endothelial Cell Marker). Reactive in Human. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD31/PECAM-1 (PECAM1), Monoclonal Antibody
Full Product Name
CD31/PECAM-1 (Endothelial Cell Marker)
Product Gene Name
Product Synonym Names
EndoCAM; PECA1; Platelet Endothelial Cell Adhesion Molecule 1; GPIIA'
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
PECAM1/3529
Host
Mouse
Species Reactivity
Human
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Specificity
CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
~100kDa (endothelium) and ~130kDa (platelets)
OMIM
173445
3D Structure
ModBase 3D Structure for P16284
Positive Control
Jurkat cells. Tonsil or Angiosarcoma.
Immunogen
Recombinant fragment (around aa 625-738) of human CD31 protein (exact sequence is proprietary)
Cellular Localization
Cell Surface and cytoplasm of endothelial cells
Hu-chromosome Location
17q23.3
Applications Tested/Suitable for anti-PECAM1 antibody
Immunohistochemistry (IHC) Paraffin
Application Notes for anti-PECAM1 antibody
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Immunohistochemistry (IHC) of anti-PECAM1 antibody

Formalin-fixed, paraffin-embedded human angiosarcoma stained with CD31 Mouse Monoclonal Antibody (PECAM1/3529).
Mouse Anti-PECAM1 Monoclonal Antibody – Cat# MBS4381158

Immunohistochemistry (IHC) of anti-PECAM1 antibody

Formalin-fixed, paraffin-embedded human tonsil stained with CD31 Mouse Monoclonal Antibody (PECAM1/3529).
Mouse Anti-PECAM1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS4381158

Immunohistochemistry (IHC) of anti-PECAM1 antibody

Formalin-fixed, paraffin-embedded human tonsil stained with CD31 Mouse Monoclonal Antibody (PECAM1/3529).
Mouse Anti-PECAM1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS4381158

SDS-Page of anti-PECAM1 antibody

SDS-PAGE Analysis Purified CD31 Mouse Monoclonal Antibody (PECAM1/3529). Confirmation of Integrity and Purity of Antibody.
Mouse Anti-PECAM1 Monoclonal Antibody, SDS-Page – Cat# MBS4381158

Testing Data of anti-PECAM1 antibody

Analysis of Protein Array containing more than 19,000 full-length human proteins using CD31 Mouse Monoclonal Antibody (PECAM1/3529) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Mouse Anti-PECAM1 Monoclonal Antibody, Testing Data – Cat# MBS4381158

Other Notes

Small volumes of anti-PECAM1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PECAM1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
platelet endothelial cell adhesion molecule
NCBI Official Synonym Full Names
platelet and endothelial cell adhesion molecule 1
NCBI Official Symbol
PECAM1
NCBI Official Synonym Symbols
CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
UniProt Protein Name
Platelet endothelial cell adhesion molecule
UniProt Synonym Protein Names
EndoCAM; GPIIA'; PECA1
UniProt Gene Name
PECAM1
UniProt Synonym Gene Names
PECAM-1
UniProt Entry Name
PECA1_HUMAN
NCBI Protein Information
platelet endothelial cell adhesion molecule

NCBI Summary for PECAM1

The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]
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UniProt Comments for PECAM1

PECAM-1: a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions. Plays a role in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, cell migration, negative regulation of immune cell signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. Undergoes alternative splicing, generating multiple isoforms in vascular beds of various tissues.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17q23.3

Cellular Component: extracellular space; platelet alpha granule membrane; integral to membrane; plasma membrane; cell junction

Molecular Function: protein binding

Biological Process: platelet activation; extracellular matrix organization and biogenesis; platelet degranulation; diapedesis; cell recognition; blood coagulation; signal transduction; cell adhesion; leukocyte migration; phagocytosis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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