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Calcineurin B/PPP3R1 (PPP3R1) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4381192

MBS4381192 is an antibody targeting PPP3R1. Also known as Calcineurin B/PPP3R1. Reactive in Human, Xenopus laevis. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Calcineurin B/PPP3R1, Monoclonal Antibody
Full Product Name
Calcineurin B/PPP3R1
Product Gene Name
anti-PPP3R1 antibody
Product Synonym Names
calcineurin B; type I (19kDa); Calcineurin subunit B type 1; CALNB1; Cna2; CNB; CNB1; Ppp3r1; protein phosphatase 3 (formerly 2B); regulatory subunit B; 19kDa; alpha isoform (calcineurin B; type I)
Clonality
Monoclonal
Isotype
IgG2a, kappa
Clone Number
BE2.1
Host
Mouse
Species Reactivity
Human, Xenopus laevis
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Specificity
Calcineurin is an enzyme that dephosphorylates serine and threonine residues in proteins. It is a heterodimer of a 59kDa catalytic A subunit and a 19kDa regulatory B subunit that is activated by the binding of calcium ions and calmodulin. Calcineurin is expressed in many tissues, but its levels are highest in the brain, where it may play a role in learning and memory. It has many substrates, including NFAT, a transcription factor that is activated by dephosphorylation. Complexes of the immuno-suppressants cyclosporine and FK506 with immunophilin proteins such as cyclophilin and FKBP12 are potent and specific inhibitors of Calcineurin activity. Alterations in Calcineurin activity are suspected to play a role in cardiac hypertrophy and graft versus host disease in organ transplantation.
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
19kDa
OMIM
601302
3D Structure
ModBase 3D Structure for P63098
Positive Control
HeLa, MCF-7 or A431 cells. Human brain, breast or stomach tissue.
Immunogen
Recombinant full-length human Calcineurin B protein
Cellular Localization
Cytoplasmic.
Hu-chromosome Location
2p14
Applications Tested/Suitable for anti-PPP3R1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-PPP3R1 antibody
ELISA: For coating, order antibody without BSA
WB: 0.5-1.0ug/ml
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Product References and Citations for anti-PPP3R1 antibody

1. Stemmer, P.M and C.B. Klee (1994) Biochemistry 33:6859. 2. Zeng H. et al. (2001) Cell 107:617

Other Notes

Small volumes of anti-PPP3R1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PPP3R1. It may not necessarily be applicable to this product.

NCBI GI #
52000904
NCBI GeneID
5534
NCBI Accession #
P63098.2
UniProt Primary Accession #
P63098
UniProt Related Accession #
P63098
NCBI Official Full Name
Calcineurin subunit B type 1
NCBI Official Synonym Full Names
protein phosphatase 3 regulatory subunit B, alpha
NCBI Official Symbol
PPP3R1
NCBI Official Synonym Symbols
CNB; CNB1; CALNB1
UniProt Protein Name
Calcineurin subunit B type 1
UniProt Synonym Protein Names
Protein phosphatase 2B regulatory subunit 1; Protein phosphatase 3 regulatory subunit B alpha isoform 1
UniProt Gene Name
PPP3R1
UniProt Synonym Gene Names
CNA2; CNB
UniProt Entry Name
CANB1_HUMAN
NCBI Protein Information
calcineurin subunit B type 1

UniProt Comments for PPP3R1

PPP3R1: Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity. Belongs to the calcineurin regulatory subunit family.

Protein type: Protein phosphatase, regulatory subunit; Cell development/differentiation; Apoptosis; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2p15

Cellular Component: nucleoplasm; cytosol; calcineurin complex; sarcolemma

Molecular Function: calmodulin binding; protein domain specific binding; protein binding; calcium-dependent protein serine/threonine phosphatase activity; calcium ion binding

Biological Process: calcineurin-NFAT signaling pathway; positive regulation of NFAT protein import into nucleus; dephosphorylation; apoptosis; innate immune response; positive regulation of transcription from RNA polymerase II promoter
Show more

Research Articles on PPP3R1

1. iTRAQ results show that let-7e is associated with the expression of anti-viral proteins. What's more, calcineurin subunit B type 1, an anti-tumor factor, is upregulated by let-7e after LPS stimulation

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PPP3R1 antibody

 Reference Product  PubMed Publications
 PPP3CB antibody  >7 publications with PPP3R1 and PPP3CB
 PPP3CA antibody  >7 publications with PPP3R1 and PPP3CA
 PPP3CC antibody  >3 publications with PPP3R1 and PPP3CC
 NFATC4 antibody  >2 publications with PPP3R1 and NFATC4
 NFATC1 antibody  >2 publications with PPP3R1 and NFATC1

Pathways associated with anti-PPP3R1 antibody

 Products by Pathway  Pathway Diagram
 Activation Of BAD And Translocation To Mitochondria Pathway antibodies  Activation Of BAD And Translocation To Mitochondria Pathway Diagram
 Activation Of BH3-only Proteins Pathway antibodies  Activation Of BH3-only Proteins Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimers Disease Pathway antibodies  Alzheimers Disease Pathway Diagram
 Amphetamine Addiction Pathway antibodies  Amphetamine Addiction Pathway Diagram
 Amphetamine Addiction Pathway antibodies  Amphetamine Addiction Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram

Diseases associated with anti-PPP3R1 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >4 publications with PPP3R1 and Nervous System Diseases
 Brain Diseases Antibodies  >4 publications with PPP3R1 and Brain Diseases
 Hypertrophy Antibodies  >3 publications with PPP3R1 and Hypertrophy
 Disease Models, Animal Antibodies  >2 publications with PPP3R1 and Disease Models, Animal
 Hypertrophy, Left Ventricular Antibodies  >2 publications with PPP3R1 and Hypertrophy, Left Ventricular
 Myocardial Ischemia Antibodies  >2 publications with PPP3R1 and Myocardial Ischemia
 Hypertension Antibodies  >1 publications with PPP3R1 and Hypertension
 Brain Injuries Antibodies  >1 publications with PPP3R1 and Brain Injuries
 Inflammation Antibodies  >1 publications with PPP3R1 and Inflammation

Organs/Tissues associated with anti-PPP3R1 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >6 publications with PPP3R1 and Muscle
 Brain Antibodies  >5 publications with PPP3R1 and Brain
 Nerve Antibodies  >2 publications with PPP3R1 and Nerve
 Bone Antibodies  >1 publications with PPP3R1 and Bone
 Liver Antibodies  >1 publications with PPP3R1 and Liver
 Spleen Antibodies  >1 publications with PPP3R1 and Spleen
 Pancreas Antibodies  >1 publications with PPP3R1 and Pancreas
 Embryonic Tissue Antibodies  >1 publications with PPP3R1 and Embryonic Tissue
 Placenta Antibodies  >1 publications with PPP3R1 and Placenta
 Lung Antibodies  >1 publications with PPP3R1 and Lung
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