Mouse Anti-SPTB Monoclonal Antibody – Cat# MBS4381267

SDS-PAGE Analysis Purified Spectrin beta III Mouse Monoclonal Antibody (RG/26). Confirmation of Purity and Integrity of Antibody.

Spectrin Beta I (SPTB) Antibody – Mouse Monoclonal

Name: SPTB Antibody; Monoclonal; Mouse; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS4381267
Price: 280 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4381267

MBS4381267 is an antibody targeting SPTB. Also known as Spectrin beta I (SPTB). Reactive in Human, Mouse, Rat. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Spectrin beta I (SPTB), Monoclonal Antibody

Full Product Name

Spectrin beta I (SPTB)

Product Gene Name

anti-SPTB antibody

Product Synonym Names

beta-I spectrin; EL3; erythrocyte; HS2; SPTB1; HSPTB1; membrane cytoskeletal protein; spectrin beta (erythrocytic)

Clonality

Monoclonal

Isotype

IgG2a, kappa

Clone Number

RG/26

Host

Mouse

Species Reactivity

Human, Mouse, Rat

Form/Format

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Purity/Purification

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Specificity

Spectrin is a cytoskeletal protein that lines the intracellular side of the plasma membrane of many cell types in pentagonal or hexagonal arrangements, forming a scaffolding and playing an important role in maintenance of plasma membrane integrity and cytoskeletal structure. In certain types of brain injury such as diffuse axonal injury, spectrin is irreversibly cleaved by the proteolytic enzyme calpain, destroying the cytosketelon. Spectrin cleavage causes the membrane to form blebs and ultimately to be degraded, usually leading to cell death.

Preparation and Storage

Antibody with azide: store at 2 to 8 degree C.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Molecular Weight

246kDa

OMIM

AB008567 mRNA

3D Structure

ModBase 3D Structure for O15020

Positive Control

Jurkat whole cell lysate (WB). Human colon carcinoma tissue (IHC).

Immunogen

Red cell membranes

Cellular Localization

Cell Surface

Hu-chromosome Location

14q23.3

Applications Tested/Suitable for anti-SPTB antibody

ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin

Application Notes for anti-SPTB antibody

ELISA: Use Ab at 2-4ug/ml for coating (Order Ab without BSA)
WB: 1-2ug/ml
IF: 1-2ug/ml
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Product References and Citations for anti-SPTB antibody

Sachanonta et al. 2008. Asian Pac J Allergy Immunol. 26(1):37-45. PMID: 18595528.

Other Notes

Small volumes of anti-SPTB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SPTB. It may not necessarily be applicable to this product.

NCBI GI #

5902122

NCBI GeneID

6712

NCBI Accession #

NP_008877.1

UniProt Primary Accession #

O15020

UniProt Related Accession #

O15020

GenBank Nucleotide #

NP_008877.1

NCBI Official Full Name

spectrin beta chain, non-erythrocytic 2

NCBI Official Synonym Full Names

spectrin beta, non-erythrocytic 2

NCBI Official Symbol

SPTBN2

NCBI Official Synonym Symbols

SCA5; SCAR14; GTRAP41

UniProt Protein Name

Spectrin beta chain, non-erythrocytic 2

UniProt Synonym Protein Names

Beta-III spectrin; Spinocerebellar ataxia 5 protein

Protein Family

Spectrin

UniProt Gene Name

SPTBN2

UniProt Synonym Gene Names

KIAA0302; SCA5

NCBI Protein Information

spectrin beta chain, non-erythrocytic 2

NCBI Summary for SPTB

Spectrins are principle components of a cell's membrane-cytoskeleton and are composed of two alpha and two beta spectrin subunits. The protein encoded by this gene (SPTBN2), is called spectrin beta non-erythrocytic 2 or beta-III spectrin. It is related to, but distinct from, the beta-II spectrin gene which is also known as spectrin beta non-erythrocytic 1 (SPTBN1). SPTBN2 regulates the glutamate signaling pathway by stabilizing the glutamate transporter EAAT4 at the surface of the plasma membrane. Mutations in this gene cause a form of spinocerebellar ataxia, SCA5, that is characterized by neurodegeneration, progressive locomotor incoordination, dysarthria, and uncoordinated eye movements. [provided by RefSeq, Dec 2009]

UniProt Comments for SPTB

Probably plays an important role in neuronal membrane skeleton.

Research Articles on SPTB

1. Study reviews the previously reported SPTBN2 mutations and cases. Moreover, the novel homozygous missense variant (c.1572C>T; p.R414C) in two brothers adds up to the literature for the infantile-onset form of autosomal recessive ataxia associated with SPTBN2.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-SPTB antibody

Products by Pathway	Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
Interaction Between L1 And Ankyrins Pathway antibodies	Interaction Between L1 And Ankyrins Pathway Diagram
L1CAM Interactions Pathway antibodies	L1CAM Interactions Pathway Diagram
NCAM Signaling For Neurite Out-growth Pathway antibodies	NCAM Signaling For Neurite Out-growth Pathway Diagram
NCAM Signaling For Neurite Out-growth Pathway antibodies	NCAM Signaling For Neurite Out-growth Pathway Diagram

Diseases associated with anti-SPTB antibody

Disease Name	Pubmed Publications
Spinocerebellar Ataxias Antibodies	>16 publications with SPTB and Spinocerebellar Ataxias
Nervous System Diseases Antibodies	>15 publications with SPTB and Nervous System Diseases
Atrophy Antibodies	>5 publications with SPTB and Atrophy
Lung Diseases Antibodies	>1 publications with SPTB and Lung Diseases

Organs/Tissues associated with anti-SPTB antibody

Organ/Tissue Name	Pubmed Publications
Nerve Antibodies	>8 publications with SPTB and Nerve
Brain Antibodies	>6 publications with SPTB and Brain
Eye Antibodies	>2 publications with SPTB and Eye
Blood Antibodies	>1 publications with SPTB and Blood
Prostate Antibodies	>1 publications with SPTB and Prostate
Liver Antibodies	>1 publications with SPTB and Liver