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Synaptophysin (MSH2) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4381277

MBS4381277 is an antibody targeting MSH2. Also known as Synaptophysin (Neuroendocrine Marker). Reactive in Human. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Synaptophysin (MSH2), Monoclonal Antibody
Full Product Name
Synaptophysin (Neuroendocrine Marker)
Product Gene Name
anti-MSH2 antibody
Product Synonym Names
Major synaptic vesicle protein p38; MRX96; MRXSYP; Syn p38; Syp; SYPH
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
SYP/3551
Host
Mouse
Species Reactivity
Human
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Specificity
This Monospecific monoclonal antibody recognizes a protein of 38kDa that is identified as synaptophysin. It is an N-glycosylated integral membrane protein found in neurons and endocrine cells. Synaptophysin contains four transmembrane domains and may function as a gap junction-like channel. This antibody identifies normal neuroendocrine cells and neuroendocrine neoplasms. Diffuse, finely granular, cytoplasmic staining is observed, which probably correlates with the distribution of the antigen within neurosecretory vesicles. Synaptophysin is an independent, broad-range marker of neural and neuroendocrine differentiation.
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
38kDa
OMIM
300802
3D Structure
ModBase 3D Structure for P08247
Positive Control
HepG2, SH-SY-5Y, HeLa or Y79 cells. Pancreas, Cerebellum or Pheochromocytoma.
Immunogen
Recombinant fragment (around aa 224-313) of human Synaptophysin (SYP) protein (exact sequence is proprietary)
Cellular Localization
Cytoplasmic
Hu-chromosome Location
Xp11.23
Applications Tested/Suitable for anti-MSH2 antibody
Immunohistochemistry (IHC) Paraffin
Application Notes for anti-MSH2 antibody
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Immunohistochemistry (IHC) of anti-MSH2 antibody

Formalin-fixed, paraffin-embedded human Pancreas stained with Synaptophysin-Monospecific Mouse Monoclonal Antibody (SYP/3551).
Mouse Anti-MSH2 Monoclonal Antibody – Cat# MBS4381277

Immunohistochemistry (IHC) of anti-MSH2 antibody

Formalin-fixed, paraffin-embedded human Pancreatic Carcinoma stained with Synaptophysin-Monospecific Mouse Monoclonal Antibody (SYP/3551).
Mouse Anti-MSH2 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS4381277

SDS-Page of anti-MSH2 antibody

SDS-PAGE Analysis Purified Synaptophysin Mouse Monoclonal Antibody (SYP/3551). Confirmation of Purity and Integrity of Antibody.
Mouse Anti-MSH2 Monoclonal Antibody, SDS-Page – Cat# MBS4381277

Testing Data of anti-MSH2 antibody

Analysis of Protein Array containing more than 19,000 full-length human proteins using Synaptophysin-Monospecific Mouse Monoclonal Antibody (SYP/3551). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Mouse Anti-MSH2 Monoclonal Antibody, Testing Data – Cat# MBS4381277

Product References and Citations for anti-MSH2 antibody

Wiedenmann B, et al. Identification and localization of synaptophysin, an integral membrane glycoprotein of Mr 38,000 characteristic of presynaptic vesicles. Cell. 1985; 41:1017-28.

Other Notes

Small volumes of anti-MSH2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MSH2. It may not necessarily be applicable to this product.

NCBI GI #
27764867
NCBI GeneID
6855
NCBI Accession #
NP_003170.1
UniProt Primary Accession #
P08247
UniProt Related Accession #
P08247
GenBank Nucleotide #
NP_003170.1
NCBI Official Full Name
synaptophysin
NCBI Official Synonym Full Names
synaptophysin
NCBI Official Symbol
SYP
NCBI Official Synonym Symbols
MRX96; MRXSYP
UniProt Protein Name
Synaptophysin
UniProt Synonym Protein Names
Major synaptic vesicle protein p38
Protein Family
DNA mismatch repair protein
UniProt Gene Name
SYP
UniProt Entry Name
SYPH_HUMAN
NCBI Protein Information
synaptophysin

NCBI Summary for MSH2

This gene encodes an integral membrane protein of small synaptic vesicles in brain and endocrine cells. The protein also binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with an X-linked form of cognitive disability. [provided by RefSeq, Jul 2017]
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UniProt Comments for MSH2

SYP: Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity. Homohexamer or homotetramer. Interacts with SRCIN1. Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype. Belongs to the synaptophysin/synaptobrevin family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Vesicle

Chromosomal Location of Human Ortholog: Xp11.23-p11.22

Cellular Component: presynaptic membrane; synaptic vesicle; neuron projection; protein complex; synaptic vesicle membrane; presynaptic active zone; excitatory synapse; cell junction; integral to synaptic vesicle membrane

Molecular Function: identical protein binding; protein self-association; transporter activity; protein complex binding; cholesterol binding; syntaxin-1 binding; SH2 domain binding

Biological Process: regulation of long-term neuronal synaptic plasticity; synaptic vesicle maturation; synaptic vesicle membrane organization and biogenesis; endocytosis; regulation of short-term neuronal synaptic plasticity; regulation of neuronal synaptic plasticity

Disease: Mental Retardation, X-linked 96
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Research Articles on MSH2

1. Using a small amount of peripheral blood,successfully detected possible neuron-derived exosome-related blood biomarkers. This is the first study to suggest that not only SYP and TNFR1 but also IL34 are important blood biomarkers for patients with Manic Depressive Disorder.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MSH2 antibody

 Reference Product  PubMed Publications
 GRB2 antibody  >42 publications with MSH2 and GRB2
 VAMP2 antibody  >4 publications with MSH2 and VAMP2

Pathways associated with anti-MSH2 antibody

 Products by Pathway  Pathway Diagram
 Synaptic Vesicle Pathway antibodies  Synaptic Vesicle Pathway Diagram

Diseases associated with anti-MSH2 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >44 publications with MSH2 and Nervous System Diseases
 Brain Diseases Antibodies  >34 publications with MSH2 and Brain Diseases
 Disease Models, Animal Antibodies  >20 publications with MSH2 and Disease Models, Animal
 Cardiovascular Diseases Antibodies  >12 publications with MSH2 and Cardiovascular Diseases
 Memory Disorders Antibodies  >9 publications with MSH2 and Memory Disorders
 Cognition Disorders Antibodies  >7 publications with MSH2 and Cognition Disorders
 Neurobehavioral Manifestations Antibodies  >7 publications with MSH2 and Neurobehavioral Manifestations
 Kidney Diseases Antibodies  >7 publications with MSH2 and Kidney Diseases
 Drug Toxicity Antibodies  >7 publications with MSH2 and Drug Toxicity
 Liver Diseases Antibodies  >6 publications with MSH2 and Liver Diseases

Organs/Tissues associated with anti-MSH2 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >101 publications with MSH2 and Brain
 Nerve Antibodies  >56 publications with MSH2 and Nerve
 Blood Antibodies  >24 publications with MSH2 and Blood
 Eye Antibodies  >9 publications with MSH2 and Eye
 Lung Antibodies  >8 publications with MSH2 and Lung
 Kidney Antibodies  >8 publications with MSH2 and Kidney
 Prostate Antibodies  >5 publications with MSH2 and Prostate
 Embryonic Tissue Antibodies  >4 publications with MSH2 and Embryonic Tissue
 Intestine Antibodies  >2 publications with MSH2 and Intestine
 Pineal Gland Antibodies  >1 publications with MSH2 and Pineal Gland
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