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C1QA Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4381295

MBS4381295 is an antibody targeting C1QA. Also known as C1QA/Complement C1q A-Chain. Reactive in Human. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
C1QA/Complement C1q A-Chain, Monoclonal Antibody
Full Product Name
C1QA/Complement C1q A-Chain
Product Gene Name
Product Synonym Names
C1QA; Complement C1q subcomponent subunit A; Complement component 1 q subcomponent A chain; Complement component 1 q subcomponent alpha polypeptide; Complement component C1q A chain
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
C1QA/2954
Host
Mouse
Species Reactivity
Human
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Specificity
C1q, a subcomponent of the classical complement pathway, is composed of nine subunits that mediate classical complement activation and thereby play an important role in the immune response. Six of these subunits are disulfide-linked dimers of chains A and B, while three of these subunits, designated C1q-A through C1q-C, are disulfide-linked dimers of chain C. Each chain contains an N-terminal collagen-like region and a C-terminal C1q globular domain. The presence of receptors for C1q on effector cells modulates its activity, which may be antibody-dependent or independent. Macrophages are the primary source of C1q, while anti-inflammatory drugs as well as cytokines differentially regulate expression of the mRNA as well as the protein. C1q deficiency is associated with lupus erythematosus and glomerulonephritis.
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
29kDa
OMIM
120550
3D Structure
ModBase 3D Structure for P02745
Positive Control
Liver or Kidney.
Immunogen
Recombinant fragment (around aa 104-237) of human C1QA protein (exact sequence is proprietary)
Cellular Localization
Secreted
Hu-chromosome Location
1p36.12
Applications Tested/Suitable for anti-C1QA antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-C1QA antibody
WB: 1-2ug/ml
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Immunohistochemistry (IHC) of anti-C1QA antibody

Formalin-fixed, paraffin-embedded human Kidney stained with C1QA Mouse Monoclonal Antibody (C1QA/2954).
Mouse Anti-C1QA Monoclonal Antibody – Cat# MBS4381295

Immunohistochemistry (IHC) of anti-C1QA antibody

Formalin-fixed, paraffin-embedded human Liver stained with C1QA Mouse Monoclonal Antibody (C1QA/2954).
Mouse Anti-C1QA Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS4381295

SDS-Page of anti-C1QA antibody

SDS-PAGE Analysis Purified C1QA Mouse Monoclonal Antibody (C1QA/2954). Confirmation of Purity and Integrity of Antibody.
Mouse Anti-C1QA Monoclonal Antibody, SDS-Page – Cat# MBS4381295

Western Blot (WB) of anti-C1QA antibody

Western Blot Analysis of human PC3 cell lysate using C1QA Mouse Monoclonal Antibody (C1QA/2954).
Mouse Anti-C1QA Monoclonal Antibody, Western Blot (WB) – Cat# MBS4381295

Testing Data of anti-C1QA antibody

Analysis of Protein Array containing more than 19,000 full-length human proteins using C1QA Mouse Monoclonal Antibody (C1QA/2954). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Mouse Anti-C1QA Monoclonal Antibody, Testing Data – Cat# MBS4381295

Product References and Citations for anti-C1QA antibody

Kishore, U., et al. 2000. C1q: structure, function and receptors. Immunopharmacology 49: 159-170.

Other Notes

Small volumes of anti-C1QA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for C1QA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
complement C1q subcomponent subunit A
NCBI Official Synonym Full Names
complement C1q A chain
NCBI Official Symbol
C1QA
UniProt Protein Name
Complement C1q subcomponent subunit A
UniProt Gene Name
C1QA
UniProt Entry Name
C1QA_HUMAN
NCBI Protein Information
complement C1q subcomponent subunit A

NCBI Summary for C1QA

This gene encodes the A-chain polypeptide of serum complement subcomponent C1q, which associates with C1r and C1s to yield the first component of the serum complement system. C1q deficiency is associated with lupus erythematosus and glomerulonephritis. C1q is composed of 18 polypeptide chains which include 6 A-chains, 6 B-chains, and 6 C-chains. Each chain contains an N-terminal collagen-like region and a C-terminal C1q globular domain. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Nov 2016]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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