Mouse Anti-ITGAX Monoclonal Antibody – Cat# MBS438163

ITGAX Antibody – Mouse Monoclonal

Name: ITGAX Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS438163
Price: 280 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS438163

MBS438163 is an antibody targeting ITGAX. Also known as CD11c. Reactive in Human.. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CD11c (ITGAX), Monoclonal Antibody

Full Product Name

CD11c

Product Gene Name

anti-ITGAX antibody

Product Synonym Names

CD11 antigen-like family member C; Complement component 3 receptor 4 subunit; Integrin alpha-X; integrin; alpha X (antigen CD11C (p150); alpha polypeptide); Leu M5 alpha subunit; Leukocyte adhesion glycoprotein p150 95 alpha chain; Myeloid membrane antigen alpha subunit; p150/95

Clonality

Monoclonal

Isotype

IgG1, kappa

Clone Number

HC1/1

Host

Mouse

Species Reactivity

Human.

Form/Format

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Specificity

CD11c, also known as Integrin, alpha X (complement component 3 receptor 4 subunit) (ITGAX), is a type I transmembrane protein found at high levels on most human dendritic cells, but also on monocytes, macrophages, neutrophils, and some B cells that induces cellular activation and helps trigger neutrophil respiratory burst; expressed in hairy cell leukemias, acute non-lymphocytic leukemias, and some B-cell chronic lymphocytic leukemias.

Preparation and Storage

Store at 2 to 8 degree C. Antibody is stable for 24 months.

Molecular Weight

145kDa

OMIM

151510

3D Structure

ModBase 3D Structure for P20702

Localization

Cell surface

Positive Control

Human dendritic cells. Human lymph nodes and tonsils.

Human Chromosomal Location

16p11.2

Immunogen

Human monocytic U937 phorbol ester-treated cells

Quantity and Buffer

0.5 ml at 200ug/ml with BSA and azide

Applications Tested/Suitable for anti-ITGAX antibody

ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Functional Studies, Immunoprecipitation (IP), Immunohistology (IH)

Application Notes for anti-ITGAX antibody

ELISA (For coating, order Ab without BSA) Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); Functional Studies (Order Ab without BSA & Azide); Immunoprecipitation (0.5-1ug/500ug protein lysate); Immunohistology (Frozen) (0.5-1.0ug/ml for 30 minutes at RT) Optimal dilution for a specific application should be determined.

Product References and Citations for anti-ITGAX antibody

1. Nicolaou, F., et al. 2003. CD11c gene expression in hairy cell leukemia is dependent upon activation of the proto-oncogenes Ras and JunD. Blood 101: 4033-4041.
2. Edwards, A.D., et al. 2003. Relationships among murine CD11c (high) dendritic cell subsets as revealed by baseline gene expression patterns. J. Immunol. 171: 47-60.

Other Notes

Small volumes of anti-ITGAX antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ITGAX. It may not necessarily be applicable to this product.

NCBI GI #

34452173

NCBI GeneID

3687

NCBI Accession #

NP_000878.2

UniProt Primary Accession #

P20702

UniProt Secondary Accession #

Q8IVA6

UniProt Related Accession #

P20702

GenBank Nucleotide #

NM_000887.4

NCBI Official Full Name

integrin alpha-X isoform 2

NCBI Official Synonym Full Names

integrin, alpha X (complement component 3 receptor 4 subunit)

NCBI Official Symbol

ITGAX

NCBI Official Synonym Symbols

CD11C; SLEB6

UniProt Protein Name

Integrin alpha-X

UniProt Synonym Protein Names

CD11 antigen-like family member C; Leu M5; Leukocyte adhesion glycoprotein p150,95 alpha chain; Leukocyte adhesion receptor p150,95; CD_antigen: CD11c

Protein Family

Integrin

UniProt Gene Name

ITGAX

UniProt Synonym Gene Names

CD11C

UniProt Entry Name

ITAX_HUMAN

NCBI Protein Information

integrin alpha-X; leu M5, alpha subunit; p150 95 integrin alpha chain; CD11 antigen-like family member C; leukocyte adhesion receptor p150,95; myeloid membrane antigen, alpha subunit; leukocyte surface antigen p150,95, alpha subunit; leukocyte adhesion glycoprotein p150,95 alpha chain; integrin, alpha X (antigen CD11C (p150), alpha polypeptide)

NCBI Summary for ITGAX

This gene encodes the integrin alpha X chain protein. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as inactivated-C3b (iC3b) receptor 4 (CR4). The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]

UniProt Comments for ITGAX

ITGAX: Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis. Belongs to the integrin alpha chain family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: cell surface; membrane; plasma membrane; integrin complex

Molecular Function: protein binding; metal ion binding; receptor activity

Biological Process: integrin-mediated signaling pathway; heterotypic cell-cell adhesion; extracellular matrix organization and biogenesis; organ morphogenesis; blood coagulation; cell adhesion; leukocyte migration

Disease: Systemic Lupus Erythematosus

Research Articles on ITGAX

1. the phosphorylation site of CD11c as Ser-1158 is pivotal for adherence and phagocytosis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ITGAX antibody

Reference Product	PubMed Publications
ITGAM antibody	>975 publications with ITGAX and ITGAM
ITGB2 antibody	>408 publications with ITGAX and ITGB2
ICAM1 antibody	>139 publications with ITGAX and ICAM1
FGG antibody	>2 publications with ITGAX and FGG

Pathways associated with anti-ITGAX antibody

Products by Pathway	Pathway Diagram
Cell Surface Interactions At The Vascular Wall Pathway antibodies	Cell Surface Interactions At The Vascular Wall Pathway Diagram
ECM Proteoglycans Pathway antibodies	ECM Proteoglycans Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram
Integrin Cell Surface Interactions Pathway antibodies	Integrin Cell Surface Interactions Pathway Diagram
Integrin-mediated Cell Adhesion Pathway antibodies	Integrin-mediated Cell Adhesion Pathway Diagram
Regulation Of Actin Cytoskeleton Pathway antibodies	Regulation Of Actin Cytoskeleton Pathway Diagram
Regulation Of Actin Cytoskeleton Pathway antibodies	Regulation Of Actin Cytoskeleton Pathway Diagram
Tuberculosis Pathway antibodies	Tuberculosis Pathway Diagram

Diseases associated with anti-ITGAX antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>503 publications with ITGAX and Inflammation
Necrosis Antibodies	>354 publications with ITGAX and Necrosis
Disease Models, Animal Antibodies	>289 publications with ITGAX and Disease Models, Animal
Lung Diseases Antibodies	>165 publications with ITGAX and Lung Diseases
Nervous System Diseases Antibodies	>115 publications with ITGAX and Nervous System Diseases
Neoplasms, Experimental Antibodies	>109 publications with ITGAX and Neoplasms, Experimental
Cardiovascular Diseases Antibodies	>104 publications with ITGAX and Cardiovascular Diseases
Vascular Diseases Antibodies	>96 publications with ITGAX and Vascular Diseases
Asthma Antibodies	>68 publications with ITGAX and Asthma
Liver Diseases Antibodies	>59 publications with ITGAX and Liver Diseases