Mouse Anti-FOXP3 Monoclonal Antibody – Cat# MBS438165

Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with FOXP3 Monoclonal Antibody (SPM579).

FOXP3 Antibody – Mouse Monoclonal

Name: FOXP3 Antibody; Monoclonal; Mouse; Human/Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS438165
Price: 280 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS438165

MBS438165 is an antibody targeting FOXP3. Also known as FOXP3 (Forkhead Box Protein P3) / Scurfin Mouse Monoclonal Antibody. Reactive in Human, Monkey, Mouse. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

FOXP3 (Forkhead Box Protein P3) / Scurfin, Monoclonal Antibody

Full Product Name

FOXP3 (Forkhead Box Protein P3) / Scurfin Mouse Monoclonal Antibody

Product Gene Name

anti-FOXP3 antibody

Product Synonym Names

JM2, AIID, DIETER, IPEX, MGC141961, MGC141963, PIDX, XPID, FOXP3delta7, forkhead box protein P3, immune dysregulation, polyendocrinopathy, enteropathy, X-linked, immunodeficiency, polyendocrinopathy, enteropathy, X-linked, scurfin

Clonality

Monoclonal

Isotype

IgG1, kappa

Clone Number

SPM579

Host

Mouse

Species Reactivity

Human, Monkey, Mouse

Form/Format

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Specificity

Recognizes a protein of 47-55kDa, which is identified as FOXP3. Its precise epitope is not known, but it has been mapped to the N-terminal portion of the protein. The FOX family of transcription factors is a large group of proteins that share a common DNA binding domain termed a winged-helix or forkhead domain. During early development, FOXP1 and FOXP2 are expressed abundantly in the lung, with lower levels of expression in neural, intestinal and cardiovascular tissues, where they act as transcription repressors. FOXP1 is widely expressed in adult tissues, while neoplastic cells often exhibit a dramatic change in expression level or localization of FOXP1. Mutations in FOXP3 gene cause IPEX, a fatal, X-linked inherited disorder characterized by immune dysregulation. The FOXP3 protein is essential for normal immune homeostasis. Specifically, FOXP3 represses transcription through a DNA binding forkhead domain, thereby regulating T cell activation.

Preparation and Storage

Antibody without azide , store at -20°C to -80°C. Non hazardous. No MSDS required.

Molecular Weight

47-55kDa

OMIM

300292

3D Structure

ModBase 3D Structure for Q9BZS1

Cellular Localization

Predominantly nuclear, some cytoplasmic

Immunogen

Full-length human FOXP3 protein

Positive Control

Tonsil, lymph node or Breast carcinoma

Human Chromosome Location

XP1123

Applications Tested/Suitable for anti-FOXP3 antibody

Flow Cytometry (FC), Immunohistochemistry (IHC) (Formalin-Fixed), Western Blot (WB)

Application Notes for anti-FOXP3 antibody

Flow Cytometry (1-2ug/million cells)
Western Blot (WB) ( 1-2ug/mL)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Product References and Citations for anti-FOXP3 antibody

Schubert, L.A., et al. 2001. Scurfin (FOXP3) acts as a repressor of transcription and regulates T cell activation. J. Biol. Chem. 276: 37672-37679

Other Notes

Small volumes of anti-FOXP3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for FOXP3. It may not necessarily be applicable to this product.

NCBI GI #

167466190

NCBI GeneID

50943

NCBI Accession #

NP_001107849.1

UniProt Primary Accession #

Q9BZS1

UniProt Secondary Accession #

O60827; Q14DD8; Q4ZH51; A5HJT1; B7ZLG0; B9UN80

UniProt Related Accession #

Q9BZS1

GenBank Nucleotide #

NM_001114377.1

NCBI Official Full Name

forkhead box protein P3 isoform b

NCBI Official Synonym Full Names

forkhead box P3

NCBI Official Symbol

FOXP3

NCBI Official Synonym Symbols

JM2; AIID; IPEX; PIDX; XPID; DIETER

UniProt Protein Name

Forkhead box protein P3

UniProt Synonym Protein Names

Scurfin

Protein Family

Forkhead box protein

UniProt Gene Name

FOXP3

UniProt Synonym Gene Names

IPEX

UniProt Entry Name

FOXP3_HUMAN

NCBI Protein Information

forkhead box protein P3

NCBI Summary for FOXP3

The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

UniProt Comments for FOXP3

FOXP3: Probable transcription factor. Plays a critical role in the control of immune response. Interacts with IKZF3. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein; Transcription factor; DNA-binding; Cell cycle regulation

Chromosomal Location of Human Ortholog: Xp11.23

Cellular Component: cytoplasm; nucleus

Molecular Function: histone acetyltransferase binding; histone deacetylase binding; NFAT protein binding; protein binding; protein homodimerization activity; sequence-specific DNA binding; transcription factor activity

Biological Process: CD4-positive, CD25-positive, alpha-beta regulatory T cell lineage commitment; inhibition of CREB transcription factor; inhibition of NF-kappaB transcription factor; negative regulation of cell proliferation; negative regulation of cytokine biosynthetic process; negative regulation of cytokine secretion; negative regulation of histone deacetylation; negative regulation of immune response; negative regulation of interferon-gamma production; negative regulation of interleukin-10 production; negative regulation of interleukin-17 production; negative regulation of interleukin-2 biosynthetic process; negative regulation of interleukin-2 production; negative regulation of interleukin-4 production; negative regulation of T cell cytokine production; negative regulation of T cell proliferation; negative regulation of transcription factor activity; negative regulation of transcription, DNA-dependent; positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation; positive regulation of histone acetylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of T cell anergy; response to virus; T cell activation

Disease: Diabetes Mellitus, Insulin-dependent; Immunodysregulation, Polyendocrinopathy, And Enteropathy, X-linked

Research Articles on FOXP3

1. A novel mutation in the FOXP3 gene affecting the Phe367 residue of the protein (F367V) is described in a family with three male siblings affected by IPEX syndrome.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-FOXP3 antibody

Reference Product	PubMed Publications
CD4 antibody	>8646 publications with FOXP3 and CD4
TNF antibody	>819 publications with FOXP3 and TNF
CTLA4 antibody	>495 publications with FOXP3 and CTLA4
TGFB1 antibody	>466 publications with FOXP3 and TGFB1
GATA3 antibody	>238 publications with FOXP3 and GATA3
IL2 antibody	>122 publications with FOXP3 and IL2
NFATC2 antibody	>20 publications with FOXP3 and NFATC2
STAT5A antibody	>17 publications with FOXP3 and STAT5A
CD8A antibody	>10 publications with FOXP3 and CD8A

Pathways associated with anti-FOXP3 antibody

Products by Pathway	Pathway Diagram
Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies	Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
IL2 Signaling Events Mediated By STAT5 Pathway antibodies	IL2 Signaling Events Mediated By STAT5 Pathway Diagram
Inflammatory Bowel Disease (IBD) Pathway antibodies	Inflammatory Bowel Disease (IBD) Pathway Diagram
Inflammatory Bowel Disease (IBD) Pathway antibodies	Inflammatory Bowel Disease (IBD) Pathway Diagram

Diseases associated with anti-FOXP3 antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>2408 publications with FOXP3 and Inflammation
Disease Models, Animal Antibodies	>1719 publications with FOXP3 and Disease Models, Animal
Necrosis Antibodies	>806 publications with FOXP3 and Necrosis
Nervous System Diseases Antibodies	>789 publications with FOXP3 and Nervous System Diseases
Lung Diseases Antibodies	>705 publications with FOXP3 and Lung Diseases
Liver Diseases Antibodies	>465 publications with FOXP3 and Liver Diseases
Cardiovascular Diseases Antibodies	>417 publications with FOXP3 and Cardiovascular Diseases
Encephalomyelitis, Autoimmune, Experimental Antibodies	>405 publications with FOXP3 and Encephalomyelitis, Autoimmune, Experimental
Diabetes Mellitus, Type 1 Antibodies	>392 publications with FOXP3 and Diabetes Mellitus, Type 1
Neoplasms, Experimental Antibodies	>328 publications with FOXP3 and Neoplasms, Experimental

Organs/Tissues associated with anti-FOXP3 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>10263 publications with FOXP3 and Blood
Lymph Node Antibodies	>1220 publications with FOXP3 and Lymph Node
Spleen Antibodies	>1200 publications with FOXP3 and Spleen
Thymus Antibodies	>828 publications with FOXP3 and Thymus
Lung Antibodies	>799 publications with FOXP3 and Lung
Eye Antibodies	>134 publications with FOXP3 and Eye
Ascites Antibodies	>20 publications with FOXP3 and Ascites
Trachea Antibodies	>11 publications with FOXP3 and Trachea
Mammary Gland Antibodies	>3 publications with FOXP3 and Mammary Gland
Uncharacterized Tissue Antibodies	>1 publications with FOXP3 and Uncharacterized Tissue