Mouse Anti-MUC5AC Monoclonal Antibody – Cat# MBS4381712

Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with Biotinylated MUC5AC Mouse Monoclonal Antibody (45M1).

MUC5AC Antibody – Mouse Monoclonal

Name: MUC5AC Antibody; Monoclonal; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS4381712
Price: 280 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4381712

MBS4381712 is an antibody targeting MUC5AC. Also known as MUC5AC (Mucin 5AC/Gastric Mucin). Reactive in Human, Monkey, Rabbit, Cat, Mouse, Rat, Pig, Hedgehog, ChickenDoes not react with Cow. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

MUC5AC, Monoclonal Antibody

Full Product Name

MUC5AC (Mucin 5AC/Gastric Mucin)

Product Gene Name

anti-MUC5AC antibody

Product Synonym Names

Apomucin Major Airway Glycoprotein; Mucin 5 subtype AC tracheobronchial; Mucin 5 Subtypes A And C; Mucin 5AC oligomeric mucus/gel forming; Tracheobronchial Mucin (TBM)

Clonality

Monoclonal

Isotype

IgG1, kappa

Clone Number

45M1

Host

Mouse

Species Reactivity

Human, Monkey, Rabbit, Cat, Mouse, Rat, Pig, Hedgehog, Chicken
Does not react with Cow

Form/Format

Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% azide and 0.05% BSA. Contact us if you require this Ab in a different format.

Purity/Purification

Purified Ab conjugated to Biotin at 1.0mg/ml

Specificity

This MAb recognizes the peptide core of gastric mucin M1 (recently identified as Mucin 5AC).Its epitope is located in the C-terminal cysteine rich part of the peptide core of MUC5AC. Its epitope is destroyed by beta-mercaptoethanol but not by periodate treatment. This mucin is present in primary ovarian mucinous cancer but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Together with a panel of antibodies, Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification of intestinal metaplasia as well as in the identification of pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.

Preparation and Storage

Antibody with azide: Store at 2 to 8 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Molecular Weight

>1000kDa

OMIM

158373

3D Structure

ModBase 3D Structure for P98088

Immunogen

M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient

Cellular Localization

Cytoplasmic

Hu-chromosome Location

11p15.5

Positive Control

Stomach

Applications Tested/Suitable for anti-MUC5AC antibody

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin

Application Notes for anti-MUC5AC antibody

FC/FACS: 2-4ug/million cells
IF: 2-4ug/ml
IHC-P: 2-4ug/ml for 30 minutes at RT
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degree C followed by cooling at RT for 20 minutes

Product References and Citations for anti-MUC5AC antibody

Cancer Res.46: 3983-3989 (1986). | Biochem. J. 254: 185-193 (1988). | Int. J. Cancer 47: 304-310 (1991).

Other Notes

Small volumes of anti-MUC5AC antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MUC5AC. It may not necessarily be applicable to this product.

NCBI GI #

160370004

NCBI GeneID

4586

NCBI Accession #

P98088.3

UniProt Primary Accession #

P98088

UniProt Related Accession #

P98088

NCBI Official Full Name

Mucin-5AC

NCBI Official Synonym Full Names

mucin 5AC, oligomeric mucus/gel-forming

NCBI Official Symbol

MUC5AC

NCBI Official Synonym Symbols

TBM; leB; MUC5; mucin

UniProt Protein Name

Mucin-5AC

UniProt Synonym Protein Names

Gastric mucin; Lewis B blood group antigen; LeB; Major airway glycoprotein; Mucin-5 subtype AC, tracheobronchial; Tracheobronchial mucin

Protein Family

Mucin

UniProt Gene Name

MUC5AC

UniProt Synonym Gene Names

MUC5; MUC-5AC; LeB; TBM

UniProt Entry Name

MUC5A_HUMAN

NCBI Protein Information

mucin-5AC

UniProt Comments for MUC5AC

MUC5AC iso1:

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: Golgi lumen; fibril

Molecular Function: extracellular matrix structural constituent

Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; fibril organization and biogenesis; post-translational protein modification

Research Articles on MUC5AC

1. In HNE-induced CRS, miR-146a downregulates the expression of MUC5AC by inhibiting the activation of EGFR, and EGFR is a target gene of miR-146a.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-MUC5AC antibody

Products by Pathway	Pathway Diagram
Metabolism Of Proteins Pathway antibodies	Metabolism Of Proteins Pathway Diagram
O-linked Glycosylation Pathway antibodies	O-linked Glycosylation Pathway Diagram
O-linked Glycosylation Of Mucins Pathway antibodies	O-linked Glycosylation Of Mucins Pathway Diagram
Post-translational Protein Modification Pathway antibodies	Post-translational Protein Modification Pathway Diagram
Termination Of O-glycan Biosynthesis Pathway antibodies	Termination Of O-glycan Biosynthesis Pathway Diagram

Organs/Tissues associated with anti-MUC5AC antibody

Organ/Tissue Name	Pubmed Publications
Stomach Antibodies	>309 publications with MUC5AC and Stomach