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CD47 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4381714

MBS4381714 is an antibody targeting CD47. Also known as CD47/IAP (Integrin Associated Protein). Reactive in Human. Host: Mouse. More details

$500 / 0.5 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD47/IAP, Monoclonal Antibody
Full Product Name
CD47/IAP (Integrin Associated Protein)
Product Gene Name
anti-CD47 antibody
Product Synonym Names
Antigenic Surface Determinant Protein OA3; IAP; Integrin Associated Protein; Integrin Associated Signal Transducer; MER6; OA3; Protein MER6; Rh Related Antigen
Clonality
Monoclonal
Isotype
IgG2c, kappa
Clone Number
CD47/2937
Host
Mouse
Species Reactivity
Human
Form/Format
Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.
Purity/Purification
Purified Ab conjugated to Biotin
Specificity
This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.
Preparation and Storage
Antibody with azide: store at 4 to 8 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
~50kDa
OMIM
601028
3D Structure
ModBase 3D Structure for Q08722
Immunogen
A recombinant fragment (around aa 18-135) of Human CD47 protein (exact sequence is proprietary)
Cellular Localization
Cell Surface
Hu-chromosome Location
3q13.12
Positive Control
MCF-7, HUVEC or OVCA3 cells (IF/FACS). Human placenta, Brain or Ovarian tumors (IHC).
Applications Tested/Suitable for anti-CD47 antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-CD47 antibody
WB: 2-4ug/ml
IHC-P: 2-4ug/ml for 30 minutes at RT
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degree C followed by cooling at RT for 20 minutes

Immunohistochemistry (IHC)-Formallin of anti-CD47 antibody

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with Biotin-conjugated CD47 Mouse Monoclonal Antibody(IAP/2937).
Mouse Anti-CD47 Monoclonal Antibody – Cat# MBS4381714

Immunohistochemistry (IHC)-Formalin of anti-CD47 antibody

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with Biotin-conjugated CD47 Mouse Monoclonal Antibody(IAP/2937).
Mouse Anti-CD47 Monoclonal Antibody, Immunohistochemistry (IHC)-Formalin – Cat# MBS4381714

SDS-Page of anti-CD47 antibody

SDS-PAGE Analysis Purified CD47 Mouse Monoclonal Antibody(IAP/2937) (unconjugated) Confirmation of Integrity and Purity of Antibody.
Mouse Anti-CD47 Monoclonal Antibody, SDS-Page – Cat# MBS4381714

Testing Data of anti-CD47 antibody

Analysis of Protein Array containing more than 19,000 full-length human proteins using CD47Mouse Monoclonal Antibody (CD47/2937) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Mouse Anti-CD47 Monoclonal Antibody, Testing Data – Cat# MBS4381714

Product References and Citations for anti-CD47 antibody

Edris Bet. al. Proc Natl Acad Sci USA 109:6656-61 (2012). | Willingham SB et. al. Proc Natl Acad Sci USA 109:6662-7 (2012).

Other Notes

Small volumes of anti-CD47 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD47. It may not necessarily be applicable to this product.

NCBI GI #
1171879
NCBI GeneID
961
NCBI Accession #
Q08722.1
UniProt Primary Accession #
Q08722
UniProt Related Accession #
Q08722
NCBI Official Full Name
Leukocyte surface antigen CD47
NCBI Official Synonym Full Names
CD47 molecule
NCBI Official Symbol
CD47
NCBI Official Synonym Symbols
IAP; OA3; MER6
UniProt Protein Name
Leukocyte surface antigen CD47
UniProt Synonym Protein Names
Antigenic surface determinant protein OA3; Integrin-associated protein; IAP; Protein MER6; CD_antigen: CD47
Protein Family
Leukocyte surface antigen
UniProt Gene Name
CD47
UniProt Synonym Gene Names
MER6; IAP
UniProt Entry Name
CD47_HUMAN
NCBI Protein Information
leukocyte surface antigen CD47

NCBI Summary for CD47

This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010]
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UniProt Comments for CD47

CD47: Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus. Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3q13.1-q13.2

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: protein binding

Biological Process: integrin-mediated signaling pathway; extracellular matrix organization and biogenesis; response to bacterium; positive regulation of phagocytosis; positive regulation of cell proliferation; positive regulation of cell-cell adhesion; positive regulation of T cell activation; opsonization; blood coagulation; cell adhesion; leukocyte migration; positive regulation of inflammatory response
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Research Articles on CD47

1. Decreased CD47 expression along the storage period was statistically significant in both groups, group 1 (unfiltered packed red cell units), and group 2 (filtered "leuco-reduced" red cell units). The expression of CD47 was significantly higher in group 2 than group 1 on day zero, 1st and 21st days, and this correlated with 2,3-DPG decreases on day 21 of group 1 but not group 2.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD47 antibody

 Reference Product  PubMed Publications
 THBS1 antibody  >141 publications with CD47 and THBS1
 SIRPA antibody  >72 publications with CD47 and SIRPA
 RHAG antibody  >22 publications with CD47 and RHAG
 PTPN11 antibody  >7 publications with CD47 and PTPN11
 SIRPB1 antibody  >5 publications with CD47 and SIRPB1
 SIRPG antibody  >3 publications with CD47 and SIRPG
 THBS2 antibody  >1 publications with CD47 and THBS2
 ITGB3 antibody  >1 publications with CD47 and ITGB3
 ITGAV antibody  >1 publications with CD47 and ITGAV

Pathways associated with anti-CD47 antibody

 Products by Pathway  Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Cell-Cell Communication Pathway antibodies  Cell-Cell Communication Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Integrin Cell Surface Interactions Pathway antibodies  Integrin Cell Surface Interactions Pathway Diagram
 Integrins In Angiogenesis Pathway antibodies  Integrins In Angiogenesis Pathway Diagram
 Signal Regulatory Protein (SIRP) Family Interactions Pathway antibodies  Signal Regulatory Protein (SIRP) Family Interactions Pathway Diagram
 Spinal Cord Injury Pathway antibodies  Spinal Cord Injury Pathway Diagram

Diseases associated with anti-CD47 antibody

 Disease Name  Pubmed Publications
 Immune System Diseases Antibodies  >84 publications with CD47 and Immune System Diseases
 Inflammation Antibodies  >81 publications with CD47 and Inflammation
 Disease Models, Animal Antibodies  >46 publications with CD47 and Disease Models, Animal
 Anemia Antibodies  >43 publications with CD47 and Anemia
 Necrosis Antibodies  >42 publications with CD47 and Necrosis
 Nervous System Diseases Antibodies  >33 publications with CD47 and Nervous System Diseases
 Brain Diseases Antibodies  >28 publications with CD47 and Brain Diseases
 Neoplasms, Experimental Antibodies  >23 publications with CD47 and Neoplasms, Experimental
 Respiratory Tract Diseases Antibodies  >14 publications with CD47 and Respiratory Tract Diseases
 Breast Neoplasms Antibodies  >10 publications with CD47 and Breast Neoplasms

Organs/Tissues associated with anti-CD47 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >434 publications with CD47 and Blood
 Vascular Antibodies  >105 publications with CD47 and Vascular
 Bone Antibodies  >75 publications with CD47 and Bone
 Bone Marrow Antibodies  >62 publications with CD47 and Bone Marrow
 Brain Antibodies  >57 publications with CD47 and Brain
 Muscle Antibodies  >54 publications with CD47 and Muscle
 Lung Antibodies  >28 publications with CD47 and Lung
 Liver Antibodies  >28 publications with CD47 and Liver
 Spleen Antibodies  >27 publications with CD47 and Spleen
 Skin Antibodies  >26 publications with CD47 and Skin
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