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Kindlin-2/KIND2 (FERMT2) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4381766

Kindlin-2 (also known as Mig-2, KIND2) is an important component of cell-extracellular matrix adhesion. Kindlin-2 binds integrins and is involved in ECM adhesion, actin assembly and cell shape modulation. This monoclonal antibody recognizes kindlin-2 in… More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Kindlin-2/KIND2 (FERMT2), Monoclonal Antibody
Full Product Name
Kindlin-2/KIND2 Mouse Monoclonal Antibody [Clone 3A3]
Product Gene Name
Product Synonym Names
Fermitin family homolog 2 (Fermt2); Kindlin-2 (KIND2); Mitogen-inducible gene 2 protein (MIG2); Pleckstrin homology domain containing family C (with FERM domain) member 1 (PLEKHC1);
Clonality
Monoclonal
Isotype
Mouse/IgG1, kappa
Clone Number
3A3
Host
Mouse
Species Reactivity
Human, Mouse, Rat, Bovine, Hamster
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0. 05% BSA & 0. 05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
Purified Ab with BSA and Azide at 200ug/ml
OR
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (lot specific)
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C
Antibody without azide: store at -20 to -80 degree C
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
72,397 Da
3D Structure
ModBase 3D Structure for Q96AC1
Immunogen
Recombinant protein containing MIG-2 (residues 218-486)
Cellular Localization
Cytoplasm
Positive Control
293T whole cell lysate. Human tonsil or liver.
Hu-chromosome Location
14q22.1
Unigene
509343
Applications Tested/Suitable for anti-FERMT2 antibody
Immunoprecipitation (IP), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry-Formalin (IHC-F)
Application Notes for anti-FERMT2 antibody
IP: 1-2ug per 100-500ug of total protein
IF: 1-2ug/ml
WB: 1-2ug/ml
IHC (Formalin-fixed): 1-2ug/ml for 30 minutes at RT
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH9.0, for 45 minutes at 95 degree C followed by cooling at RT for 20 minutes)

Immunohistochemistry (IHC)-Formalin of anti-FERMT2 antibody

Formalin-fixed, paraffin-embedded human liver stained with KIND2 Mouse Monoclonal Antibody (3A3).
Mouse Anti-FERMT2 Monoclonal Antibody – Cat# MBS4381766

Immunohistochemistry (IHC) of anti-FERMT2 antibody

Mouse Anti-FERMT2 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS4381766

Other Notes

Small volumes of anti-FERMT2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FERMT2 purchase

MBS4381766 is an antibody targeting FERMT2. Also known as Kindlin-2/KIND2 Mouse Monoclonal Antibody [Clone 3A3]. Reactive in Human, Mouse, Rat, Bovine, Hamster. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for FERMT2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
fermitin family homolog 2 isoform 2
NCBI Official Synonym Full Names
fermitin family member 2
NCBI Official Symbol
FERMT2
NCBI Official Synonym Symbols
MIG2; KIND2; mig-2; UNC112; PLEKHC1; UNC112B
UniProt Protein Name
Fermitin family homolog 2
UniProt Synonym Protein Names
Kindlin-2; Mitogen-inducible gene 2 protein; MIG-2; Pleckstrin homology domain-containing family C member 1; PH domain-containing family C member 1
Protein Family
UniProt Gene Name
FERMT2
UniProt Synonym Gene Names
KIND2; MIG2; PLEKHC1; MIG-2; PH domain-containing family C member 1
NCBI Protein Information
fermitin family homolog 2

UniProt Comments for FERMT2

Scaffolding protein that enhances integrin activation mediated by TLN1 and/or TLN2, but activates integrins only weakly by itself. Binds to membranes enriched in phosphoinositides. Enhances integrin-mediated cell adhesion onto the extracellular matrix and cell spreading; this requires both its ability to interact with integrins and with phospholipid membranes. Required for the assembly of focal adhesions. Participates in the connection between extracellular matrix adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits FBLIM1 to focal adhesions. Plays a role in the TGFB1 and integrin signaling pathways. Stabilizes active CTNNB1 and plays a role in the regulation of transcription mediated by CTNNB1 and TCF7L2/TCF4 and in Wnt signaling.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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