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CDC20 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS438179

MBS438179 is an antibody targeting CDC20. Also known as CDc20 (Cell Division Cycle Protein 20) Mouse Monoclonal Antibody. Reactive in Human. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CDc20 (Cell Division Cycle Protein 20), Monoclonal Antibody
Full Product Name
CDc20 (Cell Division Cycle Protein 20) Mouse Monoclonal Antibody
Product Gene Name
anti-CDC20 antibody
Product Synonym Names
CDC20, CDC20A, p55CDC, P55CDC-LSB
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
AR12
Host
Mouse
Species Reactivity
Human
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
Cyclins, regulatory subunits, which associate with kinases, control many of the important steps in cell cycle progression. The Cdc2 protein kinase (p34Cdc2) exhibits protein kinase activity in vitro and exists in a complex with both cyclin B and a protein homologous to p13SUC1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF) and the M phase-specific Histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition. An additional cell cycle-dependent protein kinase, termed p55cdc, exhibits a high degree of homology with the S. cerevisiae proteins Cdc20 and Cdc4. The p55cdc transcript is readily detectable in a variety of cultured cell lines in growth phase, but disappears when cell growth is chemically arrested.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
55kDa
OMIM
603618
3D Structure
ModBase 3D Structure for Q12834
Cellular Localization
Cytoplasmic
Immunogen
Urea-denatured His6 Cdc20 human recombinant protein
Hu-Chromosome Location
1p34.2
Positive Control
Ramos or HeLa cells. Tonsil or gastric carcinoma.
Applications Tested/Suitable for anti-CDC20 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
Application Notes for anti-CDC20 antibody
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Immunohistochemistry (IHC) of anti-CDC20 antibody

Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with CDC20 Monoclonal Antibody (AR12)
Mouse Anti-CDC20 Monoclonal Antibody – Cat# MBS438179

Product References and Citations for anti-CDC20 antibody

Sironi L et al. 2001. EMBO J. 20(22):6371-82.

Other Notes

Small volumes of anti-CDC20 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CDC20. It may not necessarily be applicable to this product.

NCBI GI #
118402582
NCBI GeneID
991
NCBI Accession #
NP_001246.2
UniProt Primary Accession #
Q12834
UniProt Secondary Accession #
Q5JUY4; Q9BW56; Q9UQI9; B2R6Z6; D3DPJ1
UniProt Related Accession #
Q12834
GenBank Nucleotide #
NM_001255.2
NCBI Official Full Name
cell division cycle protein 20 homolog
NCBI Official Synonym Full Names
cell division cycle 20
NCBI Official Symbol
CDC20
NCBI Official Synonym Symbols
CDC20A; p55CDC; bA276H19.3
UniProt Protein Name
Cell division cycle protein 20 homolog
UniProt Synonym Protein Names
p55CDC
Protein Family
Anaphase-promoting complex
UniProt Gene Name
CDC20
UniProt Entry Name
CDC20_HUMAN
NCBI Protein Information
cell division cycle protein 20 homolog

NCBI Summary for CDC20

CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation. [provided by RefSeq, Jul 2008]

UniProt Comments for CDC20

CDC20: Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation. Found in a complex with CDC20, CDC27, SPATC1 and TUBG1. Interacts with SPATC1. Interacts with NEUROD2. Interacts with MAD2L1 and BUB1B. The phosphorylated form interacts with APC/C. Interacts with NINL. May interact with MAD2L2. Interacts with CDK5RAP2. Interacts with isoform 1 of NEK2. Belongs to the WD repeat CDC20/Fizzy family.

Protein type: Ubiquitin conjugating system; Cell cycle regulation

Chromosomal Location of Human Ortholog: 1p34.1

Cellular Component: anaphase-promoting complex; cytoplasm; cytosol; nucleoplasm; nucleus; spindle

Molecular Function: enzyme binding; protein binding; protein C-terminus binding

Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of synaptic plasticity; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; sister chromatid cohesion
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Research Articles on CDC20

1. The presence of this segment correlates with SAC activity and efficient binding of CDC20 but not of MAD1 to kinetochores.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CDC20 antibody

 Reference Product  PubMed Publications
 CCNB1 antibody  >124 publications with CDC20 and CCNB1
 MAD2L1 antibody  >115 publications with CDC20 and MAD2L1
 BUB3 antibody  >67 publications with CDC20 and BUB3
 CDC27 antibody  >33 publications with CDC20 and CDC27
 BUB1B antibody  >23 publications with CDC20 and BUB1B
 CDC23 antibody  >19 publications with CDC20 and CDC23
 CDC16 antibody  >13 publications with CDC20 and CDC16
 ANAPC2 antibody  >8 publications with CDC20 and ANAPC2
 PTTG1 antibody  >6 publications with CDC20 and PTTG1
 ANAPC4 antibody  >5 publications with CDC20 and ANAPC4

Pathways associated with anti-CDC20 antibody

 Products by Pathway  Pathway Diagram
 APC-Cdc20 Mediated Degradation Of Nek2A Pathway antibodies  APC-Cdc20 Mediated Degradation Of Nek2A Pathway Diagram
 APC/C Complex Pathway antibodies  APC/C Complex Pathway Diagram
 APC/C Complex Pathway antibodies  APC/C Complex Pathway Diagram
 APC/C-mediated Degradation Of Cell Cycle Proteins Pathway antibodies  APC/C-mediated Degradation Of Cell Cycle Proteins Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Cyclin B Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Cyclin B Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Securin Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Securin Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
 APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway antibodies  APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway Diagram
 APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway antibodies  APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway Diagram
 Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies  Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram

Diseases associated with anti-CDC20 antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >51 publications with CDC20 and Carcinoma
 Breast Neoplasms Antibodies  >22 publications with CDC20 and Breast Neoplasms
 Adenocarcinoma Antibodies  >20 publications with CDC20 and Adenocarcinoma
 Cell Transformation, Neoplastic Antibodies  >19 publications with CDC20 and Cell Transformation, Neoplastic
 Lung Neoplasms Antibodies  >15 publications with CDC20 and Lung Neoplasms
 Lung Diseases Antibodies  >13 publications with CDC20 and Lung Diseases
 Neoplasms, Experimental Antibodies  >12 publications with CDC20 and Neoplasms, Experimental
 Liver Diseases Antibodies  >11 publications with CDC20 and Liver Diseases
 Liver Neoplasms Antibodies  >10 publications with CDC20 and Liver Neoplasms
 Prostatic Neoplasms Antibodies  >10 publications with CDC20 and Prostatic Neoplasms

Organs/Tissues associated with anti-CDC20 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >32 publications with CDC20 and Brain
 Lung Antibodies  >26 publications with CDC20 and Lung
 Liver Antibodies  >25 publications with CDC20 and Liver
 Blood Antibodies  >19 publications with CDC20 and Blood
 Prostate Antibodies  >14 publications with CDC20 and Prostate
 Bone Antibodies  >12 publications with CDC20 and Bone
 Bone Marrow Antibodies  >8 publications with CDC20 and Bone Marrow
 Muscle Antibodies  >7 publications with CDC20 and Muscle
 Kidney Antibodies  >7 publications with CDC20 and Kidney
 Nerve Antibodies  >6 publications with CDC20 and Nerve
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