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Cytokeratin 14 (KRT14) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS438196

MBS438196 is an antibody targeting KRT14. Also known as Cytokeratin 14 (KRT14) (Squamous Cell Marker) Mouse Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Cytokeratin 14 (KRT14), Monoclonal Antibody
Full Product Name
Cytokeratin 14 (KRT14) (Squamous Cell Marker) Mouse Monoclonal Antibody
Product Gene Name
anti-KRT14 antibody
Product Synonym Names
CK-14; Dowling Meara; ebs3; ebs4; Epidermolysis Bullosa Simplex; k14; Keratin 14; Keratin Type I Cytoskeletal 14; Koebner; krt14; NFJ
Clonality
Monoclonal
Isotype
IgG3
Clone Number
SPM263
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
Cytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic). CK14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. Anti-CK14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors. Anti-CK14 is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Anti-CK14 is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
50kDa
OMIM
125595
3D Structure
ModBase 3D Structure for P02533
Cellular Localization
Cytoplasmic
Immunogen
A synthetic peptide of 15 amino acid from the C-terminus of human keratin 14.
Hu-Chromosome Location
17q21.2
Positive Control
A431 cells. Skin or Squamous cell carcinoma
Applications Tested/Suitable for anti-KRT14 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
Application Notes for anti-KRT14 antibody
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Optimal dilution for a specific application should be determined.

Immunohistochemistry (IHC) of anti-KRT14 antibody

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 14 Monoclonal Antibody (SPM263).
Mouse Anti-KRT14 Monoclonal Antibody – Cat# MBS438196

Immunohistochemistry (IHC) of anti-KRT14 antibody

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 14 Monoclonal Antibody (SPM263).
Mouse Anti-KRT14 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS438196

SDS-PAGE of anti-KRT14 antibody

SDS-PAGE Analysis Purified Cytokeratin 14 MAb (SPM263). Confirmation of Integrity and Purity of Antibody.
Mouse Anti-KRT14 Monoclonal Antibody, SDS-PAGE – Cat# MBS438196

Immunofluorescence (IF) of anti-KRT14 antibody

Immunofluorescence Analysis of A549 Cells labeling KRT14 with Cytokeratin 14 MAb (SPM263) followed by Goat anti-mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).
Mouse Anti-KRT14 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS438196

Product References and Citations for anti-KRT14 antibody

Perkins W et. al. J Cutaneous Pathol, 1992, 19(6):476-82. Kasper M. Histochemistry, 1991, 95(6):613-20. Leigh I M et. al. (1988) Dermatology, 91:415

Other Notes

Small volumes of anti-KRT14 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for KRT14. It may not necessarily be applicable to this product.

NCBI GI #
15431310
NCBI GeneID
3861
NCBI Accession #
NP_000517.2
UniProt Primary Accession #
P02533
UniProt Secondary Accession #
Q14715; Q53XY3; Q9BUE3; Q9UBN2; Q9UBN3; Q9UCY4
UniProt Related Accession #
P02533
GenBank Nucleotide #
NM_000526.4
NCBI Official Full Name
keratin, type I cytoskeletal 14
NCBI Official Synonym Full Names
keratin 14
NCBI Official Symbol
KRT14
NCBI Official Synonym Symbols
K14; NFJ; CK14; EBS3; EBS4
UniProt Protein Name
Keratin, type I cytoskeletal 14
UniProt Synonym Protein Names
Cytokeratin-14; CK-14; Keratin-14; K14
Protein Family
Keratin
UniProt Gene Name
KRT14
UniProt Synonym Gene Names
CK-14; K14
UniProt Entry Name
K1C14_HUMAN
NCBI Protein Information
keratin, type I cytoskeletal 14

NCBI Summary for KRT14

This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq, Jul 2008]
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UniProt Comments for KRT14

K14: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K5.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: cytoplasm; cytosol; intermediate filament; keratin filament; nucleus

Molecular Function: protein binding; structural constituent of cytoskeleton

Biological Process: aging; epidermis development; hair cycle; hemidesmosome assembly; intermediate filament bundle assembly

Disease: Dermatopathia Pigmentosa Reticularis; Epidermolysis Bullosa Simplex, Autosomal Recessive 1; Epidermolysis Bullosa Simplex, Dowling-meara Type; Epidermolysis Bullosa Simplex, Generalized; Epidermolysis Bullosa Simplex, Localized; Naegeli Syndrome
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Research Articles on KRT14

1. all keratins tested, except for keratin 14, were evenly expressed in all trophoblast cells. Keratin 14 was expressed in a subset of CK7 positive cells

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-KRT14 antibody

 Reference Product  PubMed Publications
 KRT5 antibody  >109 publications with KRT14 and KRT5
 DST antibody  >4 publications with KRT14 and DST
 IVL antibody  >1 publications with KRT14 and IVL
 CREBBP antibody  >1 publications with KRT14 and CREBBP
 KRT3 antibody  >1 publications with KRT14 and KRT3

Pathways associated with anti-KRT14 antibody

 Products by Pathway  Pathway Diagram
 Corticotropin-releasing Hormone Pathway antibodies  Corticotropin-releasing Hormone Pathway Diagram
 Glucocorticoid Receptor Regulatory Network Pathway antibodies  Glucocorticoid Receptor Regulatory Network Pathway Diagram

Diseases associated with anti-KRT14 antibody

 Disease Name  Pubmed Publications
 Congenital Abnormalities Antibodies  >104 publications with KRT14 and Congenital Abnormalities
 Epidermolysis Bullosa Simplex Antibodies  >99 publications with KRT14 and Epidermolysis Bullosa Simplex
 Carcinoma Antibodies  >61 publications with KRT14 and Carcinoma
 Carcinoma, Squamous Cell Antibodies  >39 publications with KRT14 and Carcinoma, Squamous Cell
 Hyperplasia Antibodies  >21 publications with KRT14 and Hyperplasia
 Breast Neoplasms Antibodies  >18 publications with KRT14 and Breast Neoplasms
 Inflammation Antibodies  >15 publications with KRT14 and Inflammation
 Necrosis Antibodies  >13 publications with KRT14 and Necrosis
 Cell Transformation, Neoplastic Antibodies  >13 publications with KRT14 and Cell Transformation, Neoplastic
 Pigmentation Disorders Antibodies  >10 publications with KRT14 and Pigmentation Disorders

Organs/Tissues associated with anti-KRT14 antibody

 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >171 publications with KRT14 and Skin
 Blood Antibodies  >25 publications with KRT14 and Blood
 Mouth Antibodies  >16 publications with KRT14 and Mouth
 Lung Antibodies  >11 publications with KRT14 and Lung
 Eye Antibodies  >9 publications with KRT14 and Eye
 Mammary Gland Antibodies  >8 publications with KRT14 and Mammary Gland
 Bladder Antibodies  >8 publications with KRT14 and Bladder
 Esophagus Antibodies  >8 publications with KRT14 and Esophagus
 Liver Antibodies  >7 publications with KRT14 and Liver
 Connective Tissue Antibodies  >6 publications with KRT14 and Connective Tissue
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