Mouse Anti-MAGEA1 Monoclonal Antibody – Cat# MBS438253

Formalin-fixed, paraffin-embedded human Testis stained with MAGE-1 Monoclonal Antibody (SPM282).

MAGEA1 Antibody – Mouse Monoclonal

Name: MAGEA1 Antibody; Monoclonal; Mouse; Human/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS438253
Price: 280 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS438253

MBS438253 is an antibody targeting MAGEA1. Also known as MAGE-1 (Target for Cancer Immunotherapy) Mouse Monoclonal Antibody. Reactive in Human, Rat, Dog. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

MAGE-1 (MAGEA1), Monoclonal Antibody

Full Product Name

MAGE-1 (Target for Cancer Immunotherapy) Mouse Monoclonal Antibody

Product Gene Name

anti-MAGEA1 antibody

Product Synonym Names

MZ2 E, cancer/testis antigen 1.1, CT1.1, MAGE1A, MAGEA1, Melanoma antigen family A 1, Melanoma associated antigen 1, Melanoma associated antigen MZ2 E

Clonality

Monoclonal

Isotype

IgG1, kappa

Clone Number

SPM282

Host

Mouse

Species Reactivity

Human, Rat, Dog

Form/Format

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Specificity

Recognizes a protein of 42-46kDa, identified as MAGE-1. This MAb does not cross-react with MAGE-2, -3, -4, -6 -9, -10, -or -12 protein. Human malignant neoplasms carry rejection antigens that are recognized by the patients' autologous, tumor directed and specific, cytolytic, CD8+ T lymphocyte clones (CTL). The MAGE family of genes codes an important group of antigens. It was identified that melanomas and primary glial brain tumors express common melanoma associated antigens (MAAs). Because MAGE-1 is expressed on a significant proportion of human neoplasms of various histological types (melanoma, brain tumors of glial origin, neuroblastoma, non-small cell lung cancer, breast, gastric, colorectal, ovarian, renal cell carcinomas) and not on normal tissues, the encoded antigen may serve as a marker of early detection and target for cancer immunotherapy.

Preparation and Storage

Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Molecular Weight

42-46kDa

OMIM

300016

3D Structure

ModBase 3D Structure for P43355

Cellular Localization

Cytoplasmic

Immunogen

Human MAGE-A1 full length recombinant protein

Hu-Chromosome Location

Xq28

Positive Control

Melanoma cell lines. Melanomas, gliomas, neuroblastoma, non-small cell lung cancer, breast, gastric, colorectal, ovarian, and renal cell carcinomas.

Applications Tested/Suitable for anti-MAGEA1 antibody

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin

Application Notes for anti-MAGEA1 antibody

Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Product References and Citations for anti-MAGEA1 antibody

Qian, X et al. 2008. Mol Oncol. 2: 81-93

Other Notes

Small volumes of anti-MAGEA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MAGEA1. It may not necessarily be applicable to this product.

NCBI GI #

148276977

NCBI GeneID

4100

NCBI Accession #

NP_004979.3

UniProt Primary Accession #

P43355

UniProt Secondary Accession #

O00346; B2RC81

UniProt Related Accession #

P43355; AAB33086

GenBank Nucleotide #

NM_004988.4

NCBI Official Full Name

melanoma-associated antigen 1

NCBI Official Synonym Full Names

MAGE family member A1

NCBI Official Symbol

MAGEA1

NCBI Official Synonym Symbols

CT1.1; MAGE1

UniProt Protein Name

Melanoma-associated antigen 1

UniProt Synonym Protein Names

Antigen MZ2-E; Cancer/testis antigen 1.1; CT1.1; MAGE-1 antigen

Protein Family

Melanoma-associated antigen

UniProt Gene Name

MAGEA1

UniProt Synonym Gene Names

MAGE1; MAGE1A; CT1.1

UniProt Entry Name

MAGA1_HUMAN

NCBI Protein Information

melanoma-associated antigen 1

NCBI Summary for MAGEA1

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. [provided by RefSeq, Jul 2008]

UniProt Comments for MAGEA1

MAGE-A1: a tumor antigen expressed in a variety of tumor cells and in the testis. May play a role in embryonal development and tumor transformation or aspects of tumor progression. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.

Protein type: Cell surface

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: cytoplasm; nucleus; plasma membrane

Molecular Function: histone deacetylase binding; protein binding

Biological Process: negative regulation of Notch signaling pathway; negative regulation of transcription from RNA polymerase II promoter

Research Articles on MAGEA1

1. TRIM31 directly binds to NSE4, suggesting the existence of a TRIM31-MAGEA1-NSE4 complex reminiscent of the NSE1-NSE3-NSE4 trimer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MAGEA1 antibody

Reference Product	PubMed Publications
SMC6 antibody	>1 publications with MAGEA1 and SMC6
CTCFL antibody	>1 publications with MAGEA1 and CTCFL
MOK antibody	>1 publications with MAGEA1 and MOK
DDX43 antibody	>1 publications with MAGEA1 and DDX43

Pathways associated with anti-MAGEA1 antibody

Products by Pathway	Pathway Diagram
Delta-Notch Signaling Pathway antibodies	Delta-Notch Signaling Pathway Diagram

Diseases associated with anti-MAGEA1 antibody

Disease Name	Pubmed Publications
Melanoma Antibodies	>314 publications with MAGEA1 and Melanoma
Carcinoma Antibodies	>91 publications with MAGEA1 and Carcinoma
Adenocarcinoma Antibodies	>46 publications with MAGEA1 and Adenocarcinoma
Carcinoma, Hepatocellular Antibodies	>29 publications with MAGEA1 and Carcinoma, Hepatocellular
Head and Neck Neoplasms Antibodies	>26 publications with MAGEA1 and Head and Neck Neoplasms
Lung Neoplasms Antibodies	>25 publications with MAGEA1 and Lung Neoplasms
Carcinoma, Squamous Cell Antibodies	>23 publications with MAGEA1 and Carcinoma, Squamous Cell
Skin Neoplasms Antibodies	>18 publications with MAGEA1 and Skin Neoplasms
Sarcoma Antibodies	>18 publications with MAGEA1 and Sarcoma
Neoplasms, Experimental Antibodies	>14 publications with MAGEA1 and Neoplasms, Experimental

Organs/Tissues associated with anti-MAGEA1 antibody

Organ/Tissue Name	Pubmed Publications
Testis Antibodies	>88 publications with MAGEA1 and Testis
Lung Antibodies	>30 publications with MAGEA1 and Lung
Skin Antibodies	>20 publications with MAGEA1 and Skin
Bone Antibodies	>11 publications with MAGEA1 and Bone
Ovary Antibodies	>3 publications with MAGEA1 and Ovary
Connective Tissue Antibodies	>1 publications with MAGEA1 and Connective Tissue