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Cyclin A (CCNA2) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS438286

MBS438286 is an antibody targeting CCNA2. Also known as Cyclin A. Reactive in Human, Mouse, Cow, African Green Monkey, Mink (Mustela).. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Cyclin A (CCNA2), Monoclonal Antibody
Full Product Name
Cyclin A
Product Gene Name
anti-CCNA2 antibody
Product Synonym Names
CCN1; CCNA; CCNA2; CCNA2_HUMAN; Cyclin A2
Clonality
Monoclonal
Isotype
IgG2a, kappa
Clone Number
E67
Host
Mouse
Species Reactivity
Human, Mouse, Cow, African Green Monkey, Mink (Mustela).
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
It recognizes a protein of 54kDa, which is identified as cyclin A. Its epitope is located amino acids 144-148 of human Cyclin A2. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk's) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdk's. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while cyclin B is a critical regulator of mitosis. Mutation or disruption of normal cyclin A expression causes cells to arrest in G2-phase.
Preparation and Storage
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
54kDa
OMIM
123835
3D Structure
ModBase 3D Structure for P20248
Localization
Predominantly nuclear
Positive Control
K-562 and HeLa Cells. Human tonsil or lymph node
Human Chromosomal Location
4q27
Immunogen
Full length bovine Cyclin A protein
Applications Tested/Suitable for anti-CCNA2 antibody
ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB), Immunohistology (IH)
Application Notes for anti-CCNA2 antibody
FC: (1-2ug/million cells)
IF: (1-2ug/ml)
IHC: (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20minutes)

Optimal dilution for a specific application should be determined.

SDS-PAGE (SDS) of anti-CCNA2 antibody

SDS-PAGE Analysis Purified Cyclin A2 Mouse Monoclonal Antibody (E67). Confirmation of Integrity and Purity of Antibody.
Mouse Anti-CCNA2 Monoclonal Antibody – Cat# MBS438286

Western Blot (WB) of anti-CCNA2 antibody

Mouse Anti-CCNA2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS438286

Immunohistochemistry (IHC) of anti-CCNA2 antibody

Mouse Anti-CCNA2 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS438286

Product References and Citations for anti-CCNA2 antibody

1. Adamczewski JP; Gannon JV; Hunt T. Simian virus 40 large T antigen associates with cyclin A and p33cdk2. Journal of Virology, 1993, 67(11):6551-7.

Other Notes

Small volumes of anti-CCNA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CCNA2. It may not necessarily be applicable to this product.

NCBI GI #
4502613
NCBI GeneID
890
NCBI Accession #
NP_001228.1
UniProt Primary Accession #
P20248
UniProt Secondary Accession #
Q2M3U6; Q4W5P4; Q6LER8; A8K7B6
UniProt Related Accession #
P20248
GenBank Nucleotide #
NM_001237.3
NCBI Official Full Name
cyclin-A2
NCBI Official Synonym Full Names
cyclin A2
NCBI Official Symbol
CCNA2
NCBI Official Synonym Symbols
CCN1; CCNA
UniProt Protein Name
Cyclin-A2
Protein Family
Cyclin
UniProt Gene Name
CCNA2
UniProt Synonym Gene Names
CCN1; CCNA; Cyclin-A
UniProt Entry Name
CCNA2_HUMAN
NCBI Protein Information
cyclin-A2

NCBI Summary for CCNA2

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. [provided by RefSeq, Jul 2008]
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UniProt Comments for CCNA2

CCNA2: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.

Protein type: Nuclear receptor co-regulator; Cell cycle regulation

Chromosomal Location of Human Ortholog: 4q27

Cellular Component: nucleoplasm; male pronucleus; cytoplasm; female pronucleus; nucleus

Molecular Function: protein binding; protein kinase binding

Biological Process: mitosis; organ regeneration; response to glucagon stimulus; positive regulation of transcription, DNA-dependent; response to estradiol stimulus; positive regulation of fibroblast proliferation; cell division; mitotic cell cycle G2/M transition DNA damage checkpoint; Ras protein signal transduction; regulation of G2/M transition of mitotic cell cycle; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; G2/M transition of mitotic cell cycle
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Research Articles on CCNA2

1. cyclin A expression predicted 100% of the patients at risk for metastasis of rectal neuroendocrine tumors.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CCNA2 antibody

 Reference Product  PubMed Publications
 CDK2 antibody  >82 publications with CCNA2 and CDK2
 CDKN1A antibody  >54 publications with CCNA2 and CDKN1A
 CDK1 antibody  >51 publications with CCNA2 and CDK1
 CDKN1B antibody  >32 publications with CCNA2 and CDKN1B
 CDK4 antibody  >30 publications with CCNA2 and CDK4
 E2F1 antibody  >16 publications with CCNA2 and E2F1
 CDC20 antibody  >11 publications with CCNA2 and CDC20
 CDK6 antibody  >10 publications with CCNA2 and CDK6
 CDC6 antibody  >5 publications with CCNA2 and CDC6

Pathways associated with anti-CCNA2 antibody

 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 APC/C-mediated Degradation Of Cell Cycle Proteins Pathway antibodies  APC/C-mediated Degradation Of Cell Cycle Proteins Pathway Diagram
 ATF-2 Transcription Factor Network Pathway antibodies  ATF-2 Transcription Factor Network Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram

Diseases associated with anti-CCNA2 antibody

 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >43 publications with CCNA2 and Breast Neoplasms
 Adenocarcinoma Antibodies  >27 publications with CCNA2 and Adenocarcinoma
 Neoplasms, Experimental Antibodies  >23 publications with CCNA2 and Neoplasms, Experimental
 Lung Neoplasms Antibodies  >22 publications with CCNA2 and Lung Neoplasms
 Liver Diseases Antibodies  >20 publications with CCNA2 and Liver Diseases
 Disease Models, Animal Antibodies  >20 publications with CCNA2 and Disease Models, Animal
 Liver Neoplasms Antibodies  >19 publications with CCNA2 and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >17 publications with CCNA2 and Carcinoma, Hepatocellular
 Cardiovascular Diseases Antibodies  >13 publications with CCNA2 and Cardiovascular Diseases
 Prostatic Neoplasms Antibodies  >13 publications with CCNA2 and Prostatic Neoplasms

Organs/Tissues associated with anti-CCNA2 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >48 publications with CCNA2 and Blood
 Liver Antibodies  >47 publications with CCNA2 and Liver
 Lung Antibodies  >38 publications with CCNA2 and Lung
 Brain Antibodies  >27 publications with CCNA2 and Brain
 Muscle Antibodies  >25 publications with CCNA2 and Muscle
 Vascular Antibodies  >24 publications with CCNA2 and Vascular
 Embryonic Tissue Antibodies  >22 publications with CCNA2 and Embryonic Tissue
 Bone Antibodies  >21 publications with CCNA2 and Bone
 Testis Antibodies  >17 publications with CCNA2 and Testis
 Prostate Antibodies  >16 publications with CCNA2 and Prostate
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