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CD28 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS438303

MBS438303 is an antibody targeting CD28. Reactive in Human.. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD28, Monoclonal Antibody
Full Product Name
CD28
Product Gene Name
anti-CD28 antibody
Product Synonym Names
T-cell-specific surface glycoprotein CD28; Tp44
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
C28/75
Host
Mouse
Species Reactivity
Human.
Form/Format
supplied as 200ug/ml of AB purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide.
Specificity
Recognizes a glycoprotein of 44-88kDa, which is identified as CD28. It is the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing T-cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.
Preparation and Storage
Antibody with azide store at 2 to 8 degree C. Antibody is stable for 24 months.
Molecular Weight
44kDa (monomer); 88kDa (dimer)
OMIM
186760
3D Structure
ModBase 3D Structure for P10747
Localization
Cell surface
Positive Control
Jurkat cells or human lymph nodes and tonsils.
Human Chromosomal Location
2q33.2
Unigene
591629 (Human)
Immunogen
Recombinant human CD28 protein
Quantity and Buffer
0.5 ml at 200ug/ml with BSA and azide
Applications Tested/Suitable for anti-CD28 antibody
ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Functional Studies, Immunoprecipitation (IP), Immunohistology (IH)
Application Notes for anti-CD28 antibody
ELISA (For coating, order Ab without BSA); Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); Functional Studies (Order Ab without BSA & Azide); Immunoprecipitation (0.5-1ug/500ug protein lysate); Immunohistology (Frozen & Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 1mM EDTA Buffer, pH 8.5-9.5, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined.

Product References and Citations for anti-CD28 antibody

1. Marti F, Krause A, Post NH, Lyddane C, Dupont B, Sadelain M, King PD: Negative-feedback regulation of CD28 costimulation by a novel mitogen-activated protein kinase phosphatase, MKP6. J Immunol. 2001;166(1):197-206.
2. Scharschmidt E, Wegener E, Heissmeyer V, Rao A, Krappmann D: Degradation of Bcl10 induced by T-cell activation negatively regulates NF-kappa B signaling. Mol Cell Biol. 2004;24(9):3860-73.
3. Jeong SH, Qiao M, Nascimbeni M, Hu Z, Rehermann B, Murthy K, Liang TJ. Immunization with hepatitis C virus-like particles induces humoral and cellular immune responses in nonhuman primates. J Virol. 2004;78(13):6995-7003.

Other Notes

Small volumes of anti-CD28 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD28. It may not necessarily be applicable to this product.

NCBI GI #
340545508
NCBI GeneID
940
NCBI Accession #
NP_001230006.1
UniProt Primary Accession #
P10747
UniProt Secondary Accession #
Q13964; Q52M23; Q70WG0; Q8NI54; Q8NI55; Q8NI56; Q8WXJ2; Q9BYV0; A8KAC1
UniProt Related Accession #
P10747
GenBank Nucleotide #
NM_001243077.1
NCBI Official Full Name
T-cell-specific surface glycoprotein CD28 isoform 2
NCBI Official Synonym Full Names
CD28 molecule
NCBI Official Symbol
CD28
NCBI Official Synonym Symbols
Tp44
UniProt Protein Name
T-cell-specific surface glycoprotein CD28
UniProt Synonym Protein Names
TP44; CD_antigen: CD28
Protein Family
T-cell-specific surface glycoprotein
UniProt Gene Name
CD28
UniProt Entry Name
CD28_HUMAN
NCBI Protein Information
T-cell-specific surface glycoprotein CD28; CD28 antigen

NCBI Summary for CD28

The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]

UniProt Comments for CD28

CD28: a type I plasma membrane protein Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Binds to B7-1 and -2. Expressed in T cells and plasma cells, but not in less mature B cells. Six splice-variant isoforms have been described.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 2q33

Cellular Component: integral to plasma membrane; plasma membrane; immunological synapse; cytosol; external side of plasma membrane

Molecular Function: identical protein binding; protein binding; SH3/SH2 adaptor activity; protease binding; coreceptor activity

Biological Process: epidermal growth factor receptor signaling pathway; positive regulation of isotype switching to IgG isotypes; positive regulation of viral genome replication; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of translation; nerve growth factor receptor signaling pathway; viral reproduction; positive regulation of mitosis; cytokine biosynthetic process; positive regulation of interleukin-2 biosynthetic process; negative thymic T cell selection; T cell receptor signaling pathway; humoral immune response; regulation of defense response to virus by virus; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of inflammatory response to antigenic stimulus; positive regulation of protein kinase B signaling cascade; cell surface receptor linked signal transduction; T cell costimulation; innate immune response; positive regulation of alpha-beta T cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of T cell proliferation; regulatory T cell differentiation
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Research Articles on CD28

1. Loss of CD28 expression by liver-infiltrating T cells contributes to pathogenesis of primary sclerosing cholangitis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CD28 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 CD28 Co-stimulation Pathway antibodies  CD28 Co-stimulation Pathway Diagram
 CD28 Dependent PI3K/Akt Signaling Pathway antibodies  CD28 Dependent PI3K/Akt Signaling Pathway Diagram
 CD28 Dependent Vav1 Pathway antibodies  CD28 Dependent Vav1 Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram

Diseases associated with anti-CD28 antibody

 Disease Name  Pubmed Publications
 Autoimmune Diseases Antibodies  >1219 publications with CD28 and Autoimmune Diseases
 Necrosis Antibodies  >837 publications with CD28 and Necrosis
 Inflammation Antibodies  >781 publications with CD28 and Inflammation
 HIV Infections Antibodies  >449 publications with CD28 and HIV Infections
 Arthritis, Rheumatoid Antibodies  >354 publications with CD28 and Arthritis, Rheumatoid
 Lung Diseases Antibodies  >308 publications with CD28 and Lung Diseases
 Lymphoma Antibodies  >278 publications with CD28 and Lymphoma
 Carcinoma Antibodies  >226 publications with CD28 and Carcinoma
 Neoplasms, Experimental Antibodies  >205 publications with CD28 and Neoplasms, Experimental
 Liver Diseases Antibodies  >162 publications with CD28 and Liver Diseases

Organs/Tissues associated with anti-CD28 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >7997 publications with CD28 and Blood
 Thymus Antibodies  >337 publications with CD28 and Thymus
 Brain Antibodies  >114 publications with CD28 and Brain
 Embryonic Tissue Antibodies  >56 publications with CD28 and Embryonic Tissue
 Pharynx Antibodies  >34 publications with CD28 and Pharynx
 Connective Tissue Antibodies  >24 publications with CD28 and Connective Tissue
 Placenta Antibodies  >19 publications with CD28 and Placenta
 Uterus Antibodies  >19 publications with CD28 and Uterus
 Trachea Antibodies  >11 publications with CD28 and Trachea
 Adipose Tissue Antibodies  >7 publications with CD28 and Adipose Tissue
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