Mouse Anti-MYOD1 Monoclonal Antibody – Cat# MBS438339

Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with MyoD1 Monoclonal Antibody (SPM427)

MYOD1 Antibody – Mouse Monoclonal

Name: MYOD1 Antibody; Monoclonal; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS438339
Price: 280 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS438339

MBS438339 is an antibody targeting MYOD1. Also known as MyoD1 (Rhabdomyosarcoma Marker) Mouse Monoclonal Antibody. Reactive in Human, Mouse, Rat, Chicken. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

MyoD1, Monoclonal Antibody

Full Product Name

MyoD1 (Rhabdomyosarcoma Marker) Mouse Monoclonal Antibody

Product Gene Name

anti-MYOD1 antibody

Product Synonym Names

bHLHc1, Class C basic helix-loop-helix protein 1, Myoblast determination protein 1, Myogenic differentiation 1, Myogenic factor 3 (Myf-3), Myogenin D1, PUM

Clonality

Monoclonal

Isotype

IgG1, kappa

Clone Number

SPM427

Host

Mouse

Species Reactivity

Human, Mouse, Rat, Chicken

Form/Format

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Specificity

Recognizes a phosphor-protein of 45kDa, identified as MyoD1. The epitope of this MAb maps between amino acid 180-189 in the C-terminal of mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilms tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing's sarcomas and alveolar soft part sarcomas.

Preparation and Storage

Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Molecular Weight

45kDa

OMIM

159970

3D Structure

ModBase 3D Structure for P15172

Cellular Localization

Nuclear. Only nuclear staining should be considered as evidence of skeletal muscle differentiation.

Immunogen

Recombinant mouse MyoD1 protein

Hu-Chromosome Location

11p15.1

Positive Control

Rhabdomyosarcoma

Applications Tested/Suitable for anti-MYOD1 antibody

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin

Application Notes for anti-MYOD1 antibody

Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)

Product References and Citations for anti-MYOD1 antibody

Thulasi R et. al. Cell Growth and Differentiation, 1996, 7(4):531-41. Wesche WA et. al. American Journal of Surgical Pathology, 1995, 19(3):261-9. Parham DM et. al. Acta Neuropathologica, 1994, 87:605-11

Other Notes

Small volumes of anti-MYOD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MYOD1. It may not necessarily be applicable to this product.

NCBI GI #

23111009

NCBI GeneID

4654

NCBI Accession #

NP_002469.2

UniProt Primary Accession #

P15172

UniProt Secondary Accession #

O75321

UniProt Related Accession #

P15172

GenBank Nucleotide #

NM_002478.4

NCBI Official Full Name

myoblast determination protein 1

NCBI Official Synonym Full Names

myogenic differentiation 1

NCBI Official Symbol

MYOD1

NCBI Official Synonym Symbols

PUM; MYF3; MYOD; bHLHc1

UniProt Protein Name

Myoblast determination protein 1

UniProt Synonym Protein Names

Class C basic helix-loop-helix protein 1; bHLHc1; Myogenic factor 3; Myf-3

Protein Family

Myoblast determination protein

UniProt Gene Name

MYOD1

UniProt Synonym Gene Names

BHLHC1; MYF3; MYOD; bHLHc1; Myf-3

UniProt Entry Name

MYOD1_HUMAN

NCBI Protein Information

myoblast determination protein 1

NCBI Summary for MYOD1

This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. [provided by RefSeq, Jul 2008]

UniProt Comments for MYOD1

MyoD: a transcription factor of the basic helix-loop-helix family and the myogenic factors subfamily. Involved in muscle differentiation. Induces fibroblasts to differentiate into myoblasts. It is involved in muscle cell differentiation, and is essential for repair of damaged tissue. It activates its own transcription which may stabilize commitment to myogenesis.

Protein type: DNA-binding; Transcription factor; RNA splicing

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: cytoplasm; nuclear chromatin; nucleoplasm; nucleus; transcription factor complex

Molecular Function: chromatin binding; chromatin DNA binding; nuclear hormone receptor binding; protein binding; protein heterodimerization activity; RNA polymerase II transcription factor activity, enhancer binding; transcription coactivator activity

Biological Process: muscle cell fate commitment; muscle development; myotube cell development; positive regulation of muscle cell differentiation; positive regulation of myoblast differentiation; positive regulation of skeletal muscle fiber development; positive regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; regulation of RNA splicing; regulation of transcription from RNA polymerase II promoter; skeletal muscle development

Research Articles on MYOD1

1. These observations demonstrated the first time that Wnt3a can directly activate MyoD expression through targeting cis-elements in the DE and the L fragment.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MYOD1 antibody

Reference Product	PubMed Publications
JUN antibody	>91 publications with MYOD1 and JUN
MEF2C antibody	>22 publications with MYOD1 and MEF2C
TCF3 antibody	>20 publications with MYOD1 and TCF3
SMAD3 antibody	>10 publications with MYOD1 and SMAD3
ID1 antibody	>8 publications with MYOD1 and ID1
MEF2A antibody	>6 publications with MYOD1 and MEF2A
MYB antibody	>3 publications with MYOD1 and MYB
CREBBP antibody	>2 publications with MYOD1 and CREBBP
EP300 antibody	>2 publications with MYOD1 and EP300

Pathways associated with anti-MYOD1 antibody

Products by Pathway	Pathway Diagram
C-MYB Transcription Factor Network Pathway antibodies	C-MYB Transcription Factor Network Pathway Diagram
CDO In Myogenesis Pathway antibodies	CDO In Myogenesis Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
Id Signaling Pathway antibodies	Id Signaling Pathway Diagram
Myogenesis Pathway antibodies	Myogenesis Pathway Diagram
Notch-mediated HES/HEY Network Pathway antibodies	Notch-mediated HES/HEY Network Pathway Diagram
Regulation Of Nuclear SMAD2/3 Signaling Pathway antibodies	Regulation Of Nuclear SMAD2/3 Signaling Pathway Diagram
Regulation Of Retinoblastoma Protein Pathway antibodies	Regulation Of Retinoblastoma Protein Pathway Diagram
Signaling Events Mediated By HDAC Class III Pathway antibodies	Signaling Events Mediated By HDAC Class III Pathway Diagram
MiRs In Muscle Cell Differentiation Pathway antibodies	MiRs In Muscle Cell Differentiation Pathway Diagram

Diseases associated with anti-MYOD1 antibody

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>103 publications with MYOD1 and Nervous System Diseases
Rhabdomyosarcoma, Embryonal Antibodies	>78 publications with MYOD1 and Rhabdomyosarcoma, Embryonal
Necrosis Antibodies	>37 publications with MYOD1 and Necrosis
Inflammation Antibodies	>30 publications with MYOD1 and Inflammation
Muscular Atrophy Antibodies	>25 publications with MYOD1 and Muscular Atrophy
Hypertrophy Antibodies	>24 publications with MYOD1 and Hypertrophy
Cell Transformation, Neoplastic Antibodies	>20 publications with MYOD1 and Cell Transformation, Neoplastic
Cardiovascular Diseases Antibodies	>17 publications with MYOD1 and Cardiovascular Diseases
Lung Diseases Antibodies	>17 publications with MYOD1 and Lung Diseases
Heart Diseases Antibodies	>15 publications with MYOD1 and Heart Diseases

Organs/Tissues associated with anti-MYOD1 antibody

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>779 publications with MYOD1 and Muscle
Heart Antibodies	>50 publications with MYOD1 and Heart
Eye Antibodies	>13 publications with MYOD1 and Eye
Lymph Antibodies	>11 publications with MYOD1 and Lymph
Placenta Antibodies	>1 publications with MYOD1 and Placenta