Mouse Anti-CCNB1 Monoclonal Antibody – Cat# MBS438370

Cyclin B1 (CCNB1) Antibody – Mouse Monoclonal

Name: CCNB1 Antibody; Monoclonal; Mouse; Human/Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS438370
Price: 280 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS438370

MBS438370 is an antibody targeting CCNB1. Also known as Cyclin B1. Reactive in Human, Mouse, Hamster.. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Cyclin B1 (CCNB1), Monoclonal Antibody

Full Product Name

Cyclin B1

Product Gene Name

anti-CCNB1 antibody

Product Synonym Names

CCNB; CCNB1; CCNB1_HUMAN; G2 Mitotic Specific Cyclin B1

Clonality

Monoclonal

Isotype

IgG1, kappa

Clone Number

V92.1

Host

Mouse

Species Reactivity

Human, Mouse, Hamster.

Form/Format

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Specificity

It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.

Preparation and Storage

Store at 2 to 8 degree C. Antibody is stable for 24 months.

Molecular Weight

55-62kDa

OMIM

123836

3D Structure

ModBase 3D Structure for P14635

Localization

Cytoplasmic

Positive Control

Any human cell line in logarithmic growth phase, tonsil, or breast carcinoma

Human Chromosomal Location

5q13.2

Immunogen

Hamster Cyclin B1 protein

Quantity and Buffer

0.5 ml at 200ug/ml with BSA and azide

Applications Tested/Suitable for anti-CCNB1 antibody

ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB), Immunohistology (IH)

Application Notes for anti-CCNB1 antibody

ELISA (For coating, order antibody without BSA); Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (1-2ug/ml); Immunoprecipitation (1-2ug/500ug protein) (precipitates active CDK1/cyclin B1 complexes); Western Blotting (0.5-1ug/ml); Immunohistology (Frozen) (1-2ug/ml for 30 min at RT) Optimal dilution for a specific application should be determined.

Product References and Citations for anti-CCNB1 antibody

1. Garner AP et al. Oncogene 21:8089-104 (2002)
2. Minemoto Y et al. Exp Cell Res 262:37-48 (2001)

Other Notes

Small volumes of anti-CCNB1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CCNB1. It may not necessarily be applicable to this product.

NCBI GI #

14327896

NCBI GeneID

891

NCBI Accession #

NP_114172.1

UniProt Primary Accession #

P14635

UniProt Secondary Accession #

Q5TZP9; A8K066

UniProt Related Accession #

P14635

GenBank Nucleotide #

NM_031966.3

NCBI Official Full Name

G2/mitotic-specific cyclin-B1

NCBI Official Synonym Full Names

cyclin B1

NCBI Official Symbol

CCNB1

NCBI Official Synonym Symbols

CCNB

UniProt Protein Name

G2/mitotic-specific cyclin-B1

Protein Family

G2/mitotic-specific cyclin

UniProt Gene Name

CCNB1

UniProt Synonym Gene Names

CCNB

UniProt Entry Name

CCNB1_HUMAN

NCBI Protein Information

G2/mitotic-specific cyclin-B1; G2/mitotic-specific cyclin B1

NCBI Summary for CCNB1

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008]

UniProt Comments for CCNB1

CCNB1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Complexes with cdc2 to form the maturation-promoting factor (MPF). Two alternatively spliced isoforms have been found, a constitutively expressed form and a cell cycle-regulated form that is expressed predominantly during G2/M phase.

Protein type: Activator; Cell cycle regulation

Chromosomal Location of Human Ortholog: 5q12

Cellular Component: nucleoplasm; spindle pole; centrosome; membrane; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; histone kinase activity; patched binding; protein kinase binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of histone phosphorylation; regulation of cell cycle; ventricular cardiac muscle cell development; positive regulation of attachment of spindle microtubules to kinetochore; mitotic nuclear envelope disassembly; positive regulation of mRNA 3'-end processing; positive regulation of mitotic cell cycle; positive regulation of fibroblast proliferation; negative regulation of protein amino acid phosphorylation; tissue regeneration; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; protein complex assembly; G2/M transition of mitotic cell cycle; positive regulation of cardiac muscle cell proliferation; response to drug; in utero embryonic development; oocyte maturation; mitotic metaphase plate congression; response to DDT; gut development; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; response to mechanical stimulus; cell division; spermatogenesis; mitotic cell cycle; regulation of cyclin-dependent protein kinase activity; mitotic spindle stabilization; G1/S transition of mitotic cell cycle

Research Articles on CCNB1

1. The aim of the present study was to evaluate the prognostic value of the proliferation factors mitotic activity index (MAI), phosphohistone H3 (PPH3), cyclin B1, cyclin A and Ki67, alone and in combinations.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CCNB1 antibody

Reference Product	PubMed Publications
CDK1 antibody	>512 publications with CCNB1 and CDK1
CDC20 antibody	>101 publications with CCNB1 and CDC20
CDC27 antibody	>9 publications with CCNB1 and CDC27
CDC23 antibody	>5 publications with CCNB1 and CDC23
CDC16 antibody	>3 publications with CCNB1 and CDC16
ANAPC11 antibody	>2 publications with CCNB1 and ANAPC11
ANAPC7 antibody	>1 publications with CCNB1 and ANAPC7

Pathways associated with anti-CCNB1 antibody

Products by Pathway	Pathway Diagram
AMPK Signaling Pathway antibodies	AMPK Signaling Pathway Diagram
APC/C-mediated Degradation Of Cell Cycle Proteins Pathway antibodies	APC/C-mediated Degradation Of Cell Cycle Proteins Pathway Diagram
APC/C:Cdc20 Mediated Degradation Of Cyclin B Pathway antibodies	APC/C:Cdc20 Mediated Degradation Of Cyclin B Pathway Diagram
APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies	APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
ATM Signaling Pathway antibodies	ATM Signaling Pathway Diagram
Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies	Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
Activation Of NIMA Kinases NEK9, NEK6, NEK7 Pathway antibodies	Activation Of NIMA Kinases NEK9, NEK6, NEK7 Pathway Diagram
C-MYB Transcription Factor Network Pathway antibodies	C-MYB Transcription Factor Network Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle Checkpoints Pathway antibodies	Cell Cycle Checkpoints Pathway Diagram

Diseases associated with anti-CCNB1 antibody

Disease Name	Pubmed Publications
Adenocarcinoma Antibodies	>265 publications with CCNB1 and Adenocarcinoma
Breast Neoplasms Antibodies	>237 publications with CCNB1 and Breast Neoplasms
Lung Neoplasms Antibodies	>150 publications with CCNB1 and Lung Neoplasms
Carcinoma, Squamous Cell Antibodies	>128 publications with CCNB1 and Carcinoma, Squamous Cell
Neoplasms, Experimental Antibodies	>125 publications with CCNB1 and Neoplasms, Experimental
Liver Neoplasms Antibodies	>122 publications with CCNB1 and Liver Neoplasms
Liver Diseases Antibodies	>122 publications with CCNB1 and Liver Diseases
Carcinoma, Hepatocellular Antibodies	>108 publications with CCNB1 and Carcinoma, Hepatocellular
Prostatic Neoplasms Antibodies	>99 publications with CCNB1 and Prostatic Neoplasms
Disease Models, Animal Antibodies	>91 publications with CCNB1 and Disease Models, Animal

Organs/Tissues associated with anti-CCNB1 antibody

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>224 publications with CCNB1 and Lung
Blood Antibodies	>223 publications with CCNB1 and Blood
Liver Antibodies	>212 publications with CCNB1 and Liver
Prostate Antibodies	>134 publications with CCNB1 and Prostate
Brain Antibodies	>114 publications with CCNB1 and Brain
Vascular Antibodies	>100 publications with CCNB1 and Vascular
Muscle Antibodies	>89 publications with CCNB1 and Muscle
Bone Antibodies	>82 publications with CCNB1 and Bone
Skin Antibodies	>77 publications with CCNB1 and Skin
Ovary Antibodies	>76 publications with CCNB1 and Ovary