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CD33 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS438398

MBS438398 is an antibody targeting CD33. Reactive in Human.. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD33, Monoclonal Antibody
Full Product Name
CD33
Product Gene Name
anti-CD33 antibody
Product Synonym Names
gp67; My9; Myeloid cell surface antigen CD33; p67; Sialic acid-binding Ig-like lectin 3 (SIGLEC3)
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
WM53
Host
Mouse
Species Reactivity
Human.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
Recognizes a 67kDa glycoprotein, which is identified as CD33 (HLDA IV; WS Code M-505). CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. CD33 is expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. It is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.
Preparation and Storage
Store at 2 to 8 degree C. Antibody is stable for 24 months.
Molecular Weight
67kDa
OMIM
159590
3D Structure
ModBase 3D Structure for P20138
Localization
Cell surface
Positive Control
Human dendritic and mast cells. Human lymph nodes and tonsils.
Human Chromosomal Location
19q13.33
Immunogen
Human AML cells
Quantity and Buffer
0.5 ml at 200ug/ml with BSA and azide
Applications Tested/Suitable for anti-CD33 antibody
ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Functional Studies, Immunoprecipitation (IP), Immunohistology (IH)
Application Notes for anti-CD33 antibody
ELISA (For coating, order Ab without BSA); Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); Functional Studies (Order Ab without BSA & Azide); Immunoprecipitation (0.5-1ug/500ug protein lysate); Immunohistology (Frozen) (0.5-1.0ug/ml for 30 minutes at RT) Optimal dilution for a specific application should be determined.

Product References and Citations for anti-CD33 antibody

1. Favaloro EJ, Bradstock KF, Kabral A, Grimsley P, Berndt MC: Characterization of monoclonal antibodies to the human myeloid-differentiation antigen, 'gp67' (CD-33). Dis Markers. 1987;5(4):215-25.
2. Favaloro EJ, Bradstock KF, Kabral A, Grimsley P, Zowtyj H, Zola H: Further characterization of human myeloid antigens (gp160,95; gp150; gp67): investigation of epitopic heterogeneity and non-haemopoietic distribution using panels of monoclonal antibodies belonging to CD-11b, CD-13 and CD-33. Br J Haematol. 1988;69(2):163-71.

Other Notes

Small volumes of anti-CD33 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD33. It may not necessarily be applicable to this product.

NCBI GI #
130980072
NCBI GeneID
945
NCBI Accession #
NP_001076087.1
UniProt Primary Accession #
P20138
UniProt Secondary Accession #
Q8TD24; B4E3P8; C9JEN7; F8WAL2
UniProt Related Accession #
P20138
GenBank Nucleotide #
NM_001082618.1
NCBI Official Full Name
myeloid cell surface antigen CD33 isoform 2
NCBI Official Synonym Full Names
CD33 molecule
NCBI Official Symbol
CD33
NCBI Official Synonym Symbols
p67; SIGLEC3; SIGLEC-3
UniProt Protein Name
Myeloid cell surface antigen CD33
UniProt Synonym Protein Names
Sialic acid-binding Ig-like lectin 3; Siglec-3; gp67; CD_antigen: CD33
Protein Family
Myeloid cell surface antigen
UniProt Gene Name
CD33
UniProt Synonym Gene Names
SIGLEC3; Siglec-3
UniProt Entry Name
CD33_HUMAN
NCBI Protein Information
myeloid cell surface antigen CD33; gp67; CD33 antigen (gp67); sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3

UniProt Comments for CD33

CD33: Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro). Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: protein binding; receptor activity; carbohydrate binding

Biological Process: negative regulation of cell proliferation; cell-cell signaling; signal transduction; cell adhesion
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Research Articles on CD33

1. Case Report: indolent T-lymphoblastic proliferation with disseminated multinodal involvement with partial CD33 expression on T cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD33 antibody

 Reference Product  PubMed Publications
 CD34 antibody  >1056 publications with CD33 and CD34
 SRC antibody  >19 publications with CD33 and SRC
 PTPN11 antibody  >11 publications with CD33 and PTPN11
 PTPN6 antibody  >7 publications with CD33 and PTPN6
 CBL antibody  >3 publications with CD33 and CBL
 UBC antibody  >3 publications with CD33 and UBC
 PVR antibody  >1 publications with CD33 and PVR

Pathways associated with anti-CD33 antibody

 Products by Pathway  Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram

Diseases associated with anti-CD33 antibody

 Disease Name  Pubmed Publications
 Leukemia, Myeloid Antibodies  >1313 publications with CD33 and Leukemia, Myeloid
 Leukemia, Myeloid, Acute Antibodies  >1151 publications with CD33 and Leukemia, Myeloid, Acute
 Necrosis Antibodies  >92 publications with CD33 and Necrosis
 Cardiovascular Diseases Antibodies  >77 publications with CD33 and Cardiovascular Diseases
 Inflammation Antibodies  >66 publications with CD33 and Inflammation
 Respiratory Tract Diseases Antibodies  >43 publications with CD33 and Respiratory Tract Diseases
 Breast Neoplasms Antibodies  >38 publications with CD33 and Breast Neoplasms
 Brain Diseases Antibodies  >33 publications with CD33 and Brain Diseases
 Leukocytosis Antibodies  >32 publications with CD33 and Leukocytosis
 Lung Diseases Antibodies  >27 publications with CD33 and Lung Diseases

Organs/Tissues associated with anti-CD33 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1398 publications with CD33 and Blood
 Bone Antibodies  >976 publications with CD33 and Bone
 Bone Marrow Antibodies  >965 publications with CD33 and Bone Marrow
 Liver Antibodies  >83 publications with CD33 and Liver
 Lymph Node Antibodies  >53 publications with CD33 and Lymph Node
 Lung Antibodies  >46 publications with CD33 and Lung
 Brain Antibodies  >29 publications with CD33 and Brain
 Thymus Antibodies  >28 publications with CD33 and Thymus
 Kidney Antibodies  >18 publications with CD33 and Kidney
 Connective Tissue Antibodies  >10 publications with CD33 and Connective Tissue
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