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HSP27 (Heat Shock Protein 27) (HSPB1) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS438416

MBS438416 is an antibody targeting HSPB1. Also known as HSP27 (Heat Shock Protein 27) Mouse Monoclonal Antibody. Reactive in Human, Chimpanzee, Monkey, Sheep, Rat, Mouse, Chicken. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
HSP27 (Heat Shock Protein 27) (HSPB1), Monoclonal Antibody
Full Product Name
HSP27 (Heat Shock Protein 27) Mouse Monoclonal Antibody
Product Gene Name
anti-HSPB1 antibody
Product Synonym Names
Heat shock 27kDa protein, Estrogen-regulated 24kDa protein, Heat shock 25kDa protein 1, Heat shock 28kDa protein 1, Heat shock protein beta-1, HMN2B, Hsp25, Hsp28, HspB1, Stress-responsive protein 27 (SRP27)
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
G3.1
Host
Mouse
Species Reactivity
Human, Chimpanzee, Monkey, Sheep, Rat, Mouse, Chicken
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Specificity
This MAb reacts specifically with heat shock protein HSP27 in human and monkey tissues and cell lines such as MCF-7. HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSPs and mammalian alpha-crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore HSP27 may be useful in classifying various hormone sensitive tumors.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
27kDa
OMIM
602195
3D Structure
ModBase 3D Structure for P04792
Cellular Localization
Predominantly Cytoplasmic with some nuclear
Immunogen
Partially purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells.
Hu-Chromosome Location
7q11.23
Positive Control
~50% of Breast Carcinomas are positive for hsp27 especially those that are also positive for ER and/or PgR.
Applications Tested/Suitable for anti-HSPB1 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin
Application Notes for anti-HSPB1 antibody
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Western Blot (0.25-0.5ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Immunohistochemistry (IHC) of anti-HSPB1 antibody

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with HSP27 Monoclonal Antibody (G3.1)
Mouse Anti-HSPB1 Monoclonal Antibody – Cat# MBS438416

Immunohistochemistry (IHC) of anti-HSPB1 antibody

Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with HSP27 Monoclonal Antibody (G3.1)
Mouse Anti-HSPB1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS438416

Product References and Citations for anti-HSPB1 antibody

Edwards DP et. al. Biochem Biophys Research Commun, 93:804-812, 1980. Ciocca DR et. al. Breast Cancer Research and Treatment, 20:33-42, 1991

Other Notes

Small volumes of anti-HSPB1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HSPB1. It may not necessarily be applicable to this product.

NCBI GI #
4504517
NCBI GeneID
3315
NCBI Accession #
NP_001531.1
UniProt Primary Accession #
P04792
UniProt Secondary Accession #
Q6FI47; Q96C20; Q96EI7; Q9UC31; Q9UC34; Q9UC35; Q9UC36; B2R4N8
UniProt Related Accession #
P04792
GenBank Nucleotide #
NM_001540.3
NCBI Official Full Name
heat shock protein beta-1
NCBI Official Synonym Full Names
heat shock protein family B (small) member 1
NCBI Official Symbol
HSPB1
NCBI Official Synonym Symbols
CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
UniProt Protein Name
Heat shock protein beta-1
UniProt Synonym Protein Names
28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27
Protein Family
Heat shock protein
UniProt Gene Name
HSPB1
UniProt Synonym Gene Names
HSP27; HSP28; HspB1; HSP 27; SRP27
UniProt Entry Name
HSPB1_HUMAN
NCBI Protein Information
heat shock protein beta-1

NCBI Summary for HSPB1

The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008]
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UniProt Comments for HSPB1

HSP27: a small heat shock protein that is regulated both transcriptionally and posttranslationally. Modulates actin polymerization and reorganization. Its expression level increases several-fold in response to stress and is phosphorylated by MAPKAP kinase 2. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock.

Protein type: Heat shock protein; Motility/polarity/chemotaxis; Chaperone

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: cytoplasm; cytoskeleton; cytosol; extracellular space; focal adhesion; nucleus; proteasome complex

Molecular Function: identical protein binding; protein binding; protein kinase binding; protein kinase C binding; protein kinase C inhibitor activity; ubiquitin binding

Biological Process: cell motility; negative regulation of apoptosis; negative regulation of protein kinase activity; positive regulation of angiogenesis; positive regulation of blood vessel endothelial cell migration; positive regulation of interleukin-1 beta production; positive regulation of tumor necrosis factor biosynthetic process; regulation of I-kappaB kinase/NF-kappaB cascade; regulation of mRNA stability; regulation of translational initiation; response to virus; retinal homeostasis; vascular endothelial growth factor receptor signaling pathway

Disease: Charcot-marie-tooth Disease, Axonal, Type 2f; Neuronopathy, Distal Hereditary Motor, Type Iib
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Research Articles on HSPB1

1. Data show there is an inverse correlation between heat shock protein 27 (HSP27) expression and gemcitabine (GEM) sensitivity of pancreatic cancer SW1990 cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-HSPB1 antibody

 Reference Product  PubMed Publications
 MAPKAPK2 antibody  >58 publications with HSPB1 and MAPKAPK2
 HSF1 antibody  >49 publications with HSPB1 and HSF1
 HSP90AA1 antibody  >7 publications with HSPB1 and HSP90AA1
 TP53 antibody  >7 publications with HSPB1 and TP53
 MAPK14 antibody  >7 publications with HSPB1 and MAPK14
 AKT1 antibody  >6 publications with HSPB1 and AKT1
 MAPKAPK5 antibody  >5 publications with HSPB1 and MAPKAPK5
 MAPKAPK3 antibody  >4 publications with HSPB1 and MAPKAPK3
 UBC antibody  >3 publications with HSPB1 and UBC
 EIF4G1 antibody  >1 publications with HSPB1 and EIF4G1

Pathways associated with anti-HSPB1 antibody

 Products by Pathway  Pathway Diagram
 AUF1 (hnRNP D0) Binds And Destabilizes MRNA Pathway antibodies  AUF1 (hnRNP D0) Binds And Destabilizes MRNA Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 FAS Pathway And Stress Induction Of HSP Regulation antibodies  FAS Pathway And Stress Induction Of HSP Regulation Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 IL-3 Signaling Pathway antibodies  IL-3 Signaling Pathway Diagram
 IL-6 Signaling Pathway antibodies  IL-6 Signaling Pathway Diagram
 MAPK Family Signaling Cascades Pathway antibodies  MAPK Family Signaling Cascades Pathway Diagram

Diseases associated with anti-HSPB1 antibody

 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >106 publications with HSPB1 and Disease Models, Animal
 Inflammation Antibodies  >75 publications with HSPB1 and Inflammation
 Necrosis Antibodies  >72 publications with HSPB1 and Necrosis
 Breast Neoplasms Antibodies  >69 publications with HSPB1 and Breast Neoplasms
 Heart Diseases Antibodies  >64 publications with HSPB1 and Heart Diseases
 Liver Diseases Antibodies  >44 publications with HSPB1 and Liver Diseases
 Reperfusion Injury Antibodies  >38 publications with HSPB1 and Reperfusion Injury
 Kidney Diseases Antibodies  >35 publications with HSPB1 and Kidney Diseases
 Lung Neoplasms Antibodies  >34 publications with HSPB1 and Lung Neoplasms
 Carcinoma, Squamous Cell Antibodies  >30 publications with HSPB1 and Carcinoma, Squamous Cell

Organs/Tissues associated with anti-HSPB1 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >242 publications with HSPB1 and Muscle
 Blood Antibodies  >181 publications with HSPB1 and Blood
 Brain Antibodies  >173 publications with HSPB1 and Brain
 Heart Antibodies  >123 publications with HSPB1 and Heart
 Nerve Antibodies  >115 publications with HSPB1 and Nerve
 Vascular Antibodies  >104 publications with HSPB1 and Vascular
 Liver Antibodies  >86 publications with HSPB1 and Liver
 Lung Antibodies  >81 publications with HSPB1 and Lung
 Kidney Antibodies  >78 publications with HSPB1 and Kidney
 Eye Antibodies  >49 publications with HSPB1 and Eye
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