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NKX2-2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS438419

MBS438419 is an antibody targeting NKX2-2. Also known as NKX2.2 (Neuroendocrine & Ewing Sarcoma Marker) Mouse Monoclonal Antibody. Reactive in Human, Mouse, Rat, Chicken. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NKX2.2 (NKX2-2), Monoclonal Antibody
Full Product Name
NKX2.2 (Neuroendocrine & Ewing Sarcoma Marker) Mouse Monoclonal Antibody
Product Gene Name
Product Synonym Names
Homeobox protein NK-2 homolog B, NK2 transcription factor like protein B, NK2 transcription factor related locus 2, NKX22, Nkx2b, tinman
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
NX2/294
Host
Mouse
Species Reactivity
Human, Mouse, Rat, Chicken
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
Expression of NKX2.2 has been found in neuroendocrine tumors of the gut, making it a potential marker for the study of gastrointestinal neuroendocrine tumors. More recently, NKX2.2 protein was identified as a target of EWS-FLI-1, the fusion protein specific to Ewing'sarcoma, and was shown to be differentially upregulated in Ewing'sarcoma on the basis of array-based gene expression analysis. It acts as a valuable marker for Ewing'sarcoma, with a sensitivity of 93% and a specificity of 89%, and aids in the differential small round cell tumors.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
40-50kDa
OMIM
604612
3D Structure
ModBase 3D Structure for O95096
Cellular Localization
Nuclear
Immunogen
Human full-length recombinant NKX2.2 protein
Hu-Chromosome Location
20p11.22
Positive Control
Pancreas or Ewings sarcoma
Applications Tested/Suitable for anti-NKX2-2 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
Application Notes for anti-NKX2-2 antibody
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Immunohistochemistry (IHC) of anti-NKX2-2 antibody

Formalin-fixed, paraffin-embedded human Ewing's Sarcoma stained with NKX2.2 Monoclonal Antibody (NX2/294).
Mouse Anti-NKX2-2 Monoclonal Antibody – Cat# MBS438419

Immunohistochemistry (IHC) of anti-NKX2-2 antibody

Formalin-fixed, paraffin-embedded human Pancreas stained with NKX2.2 Monoclonal Antibody (NX2/294).
Mouse Anti-NKX2-2 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS438419

Immunohistochemistry (IHC) of anti-NKX2-2 antibody

Formalin-fixed, paraffin-embedded Rat Pancreas stained with NKX2.2 Monoclonal Antibody (NX2/294).
Mouse Anti-NKX2-2 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS438419

Product References and Citations for anti-NKX2-2 antibody

Wang, Y.C. et al. (2009) Endocr Relat Cancer 16, 267-79. Yoshida A et. al. Am J Surg Pathol. 2012;36(7):993-9.

Other Notes

Small volumes of anti-NKX2-2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NKX2-2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
homeobox protein Nkx-2.2
NCBI Official Synonym Full Names
NK2 homeobox 2
NCBI Official Symbol
NKX2-2
NCBI Official Synonym Symbols
NKX2B; NKX2.2
UniProt Protein Name
Homeobox protein Nkx-2.2
UniProt Synonym Protein Names
Homeobox protein NK-2 homolog B
Protein Family
UniProt Gene Name
NKX2-2
UniProt Synonym Gene Names
NKX2.2; NKX2B
UniProt Entry Name
NKX22_HUMAN
NCBI Protein Information
homeobox protein Nkx-2.2

NCBI Summary for NKX2-2

The protein encoded by this gene contains a homeobox domain and may be involved in the morphogenesis of the central nervous system. This gene is found on chromosome 20 near NKX2-4, and these two genes appear to be duplicated on chromosome 14 in the form of TITF1 and NKX2-8. The encoded protein is likely to be a nuclear transcription factor. [provided by RefSeq, Jul 2008]
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UniProt Comments for NKX2-2

NKX2-2: Acts as a transcriptional activator. Required for the maintenance of NEUROD1 expression in the horomone-producing endocrine cells of the pancreas. May be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. Associates with chromatin at the NEUROD1 promoter region. Binds to a subset of consensus elements within the NEUROD1 promoter. Belongs to the NK-2 homeobox family.

Protein type: Cell development/differentiation; Transcription factor

Chromosomal Location of Human Ortholog: 20p11.22

Cellular Component: nucleoplasm

Molecular Function: chromatin binding; transcription coactivator activity

Biological Process: brain development; endocrine pancreas development; positive regulation of transcription factor activity
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-NKX2-2 antibody

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