Thymidylate Synthase (TYMS) Antibody – Mouse Monoclonal

Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase. [provided by RefSeq, Jul 2008]

TYMS: an enzyme in the thymidylate synthase family. Polymorphisms serve as prognostic markers for determining modality of treatment in certain cancers. Genotyping helps predict toxicity to 5-FU-based chemotherapy in patients with colorectal cancer and the response to platinum-based chemotherapy in advanced NSCLC. A common polymorphism is a determinant of red blood cell folate and homocysteine concentrations. Homodimer.

Protein type: Methyltransferase; DNA repair, damage; Nucleotide Metabolism - pyrimidine; Cofactor and Vitamin Metabolism - one carbon pool by folate; EC 2.1.1.45; Mitochondrial

Chromosomal Location of Human Ortholog: 18p11.32

Cellular Component: cytoplasm; cytosol; mitochondrial inner membrane; mitochondrial matrix; mitochondrion; nucleoplasm; nucleus

Molecular Function: folic acid binding; thymidylate synthase activity

Biological Process: deoxyribonucleoside monophosphate biosynthetic process; dTMP biosynthetic process; G1/S transition of mitotic cell cycle; G1/S-specific transcription in mitotic cell cycle; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; pyrimidine nucleoside biosynthetic process; response to organophosphorus