• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

CD57 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS438535

MBS438535 is an antibody targeting CD57. Also known as CD57 / B3GAT1 (Natural Killer Cell Marker) Mouse Monoclonal Antibody. Reactive in Human.Does not react with rat. Others not known.. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD57 / B3GAT1, Monoclonal Antibody
Full Product Name
CD57 / B3GAT1 (Natural Killer Cell Marker) Mouse Monoclonal Antibody
Product Gene Name
anti-CD57 antibody
Product Synonym Gene Name
B3GAT1
Product Synonym Names
3-Glucuronyltransferase 1; B3GAT1; Galactosylgalactosylxylosylprotein 3-beta-Glucuronosyltransferase 1; GLCATP; GlcUAT-P; Glucuronosyltransferase P; UDP GlcUA Glycoprotein beta 1, 3 Glucuronyltransferase
Clonality
Monoclonal
Isotype
Mouse / IgM, kappa (HNK-1) + Mouse / IgM, kappa (NK-1)
Clone Number
HNK-1 + NK-1
Host
Mouse
Species Reactivity
Human.
Does not react with rat. Others not known.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide.
Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
Anti-CD57 marks a subset of lymphocytes known as natural killer (NK) cells. Follicular center cell lymphomas often contain many NK cells within the neoplastic follicles. Anti-CD57 also stains neuroendocrine cells and their derived tumors, including carcinoid tumor and medulloblastoma. Anti-CD57 can also be useful in separating type B3 thymoma from thymic carcinoma when combined with a panel that includes antibodies against GLUT1, CD5, and CEA.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
~110kDa
OMIM
151290
3D Structure
ModBase 3D Structure for Q9P2W7
Cellular Localization
Cell Surface
Immunogen
Human peripheral blood mononuclear cells (HNK-1 & NK-1)
Hu-Chromosome Location
11q25
Positive Control
Lymph node or Tonsil
Applications Tested/Suitable for anti-CD57 antibody
Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
Application Notes for anti-CD57 antibody
Immunofluorescence (0.5-1ug/ml)
Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)

Optimal dilution for a specific application should be determined.

Immunohistochemistry (IHC) of anti-CD57 antibody

Formalin-fixed, paraffin-embedded human Tonsil stained with CD57 Monoclonal Antibody (HNK-1 + NK-1).
Mouse Anti-CD57 Monoclonal Antibody – Cat# MBS438535

Product References and Citations for anti-CD57 antibody

Abo T et. al. J Immunol, 1982, 129(4):1758-61. Abo T et al. J Immunology, 1982, 129:1752-7

Other Notes

Small volumes of anti-CD57 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD57. It may not necessarily be applicable to this product.

NCBI GI #
77695914
NCBI GeneID
27087
NCBI Accession #
NP_061114.2
UniProt Primary Accession #
Q9P2W7
UniProt Secondary Accession #
Q96FS7
UniProt Related Accession #
Q9P2W7
GenBank Nucleotide #
NM_018644.3
NCBI Official Full Name
galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1
NCBI Official Synonym Full Names
beta-1,3-glucuronyltransferase 1
NCBI Official Symbol
B3GAT1
NCBI Official Synonym Symbols
NK1; CD57; HNK1; LEU7; NK-1; GLCATP; GLCUATP
UniProt Protein Name
Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1
UniProt Synonym Protein Names
Beta-1,3-glucuronyltransferase 1; Glucuronosyltransferase P; GlcAT-P; UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase; GlcUAT-P
UniProt Gene Name
B3GAT1
UniProt Synonym Gene Names
GLCATP; GlcAT-P; GlcUAT-P
UniProt Entry Name
B3GA1_HUMAN
NCBI Protein Information
galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1

NCBI Summary for CD57

The protein encoded by this gene is a member of the glucuronyltransferase gene family. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1, also known as CD57 and LEU7). Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]
Show more

UniProt Comments for CD57

B3GAT1: Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo- orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl- sphingomyelin was the most effective, followed by palmitoyl- sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group. Belongs to the glycosyltransferase 43 family.

Protein type: Transferase; Membrane protein, integral; EC 2.4.1.135; Glycan Metabolism - heparan sulfate biosynthesis; Glycan Metabolism - chondroitin sulfate biosynthesis

Chromosomal Location of Human Ortholog: 11q25

Cellular Component: Golgi membrane

Molecular Function: galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity

Biological Process: carbohydrate metabolic process; chondroitin sulfate metabolic process; chondroitin sulfate proteoglycan biosynthetic process; glycosaminoglycan metabolic process
Show more

Research Articles on CD57

1. Case Report: chronic lymphocytic leukemia with aberrant CD56 and CD57 expression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CD57 antibody

 Products by Pathway  Pathway Diagram
 A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway antibodies  A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway Diagram
 Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway antibodies  Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway Diagram
 Glycosaminoglycan Metabolism Pathway antibodies  Glycosaminoglycan Metabolism Pathway Diagram
 Heparan Sulfate/heparin (HS-GAG) Metabolism Pathway antibodies  Heparan Sulfate/heparin (HS-GAG) Metabolism Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Carbohydrates Pathway antibodies  Metabolism Of Carbohydrates Pathway Diagram
 Other Types Of O-glycan Biosynthesis Pathway antibodies  Other Types Of O-glycan Biosynthesis Pathway Diagram
 Other Types Of O-glycan Biosynthesis Pathway antibodies  Other Types Of O-glycan Biosynthesis Pathway Diagram
 Chondroitin Sulfate Biosynthesis Pathway antibodies  Chondroitin Sulfate Biosynthesis Pathway Diagram
 Chondroitin Sulfate Biosynthesis Pathway antibodies  Chondroitin Sulfate Biosynthesis Pathway Diagram

Diseases associated with anti-CD57 antibody

 Disease Name  Pubmed Publications
 Atrophy Antibodies  >1 publications with CD57 and Atrophy
 Prenatal Injuries Antibodies  >1 publications with CD57 and Prenatal Injuries
 Necrosis Antibodies  >1 publications with CD57 and Necrosis
 Prenatal Exposure Delayed Effects Antibodies  >1 publications with CD57 and Prenatal Exposure Delayed Effects

Organs/Tissues associated with anti-CD57 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >6 publications with CD57 and Brain
 Nerve Antibodies  >5 publications with CD57 and Nerve
 Blood Antibodies  >2 publications with CD57 and Blood
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000