Mouse Anti-KRT10 Monoclonal Antibody – Cat# MBS438606

Formalin-fixed, paraffin-embedded human Tonsil stained with Cytokeratin 10/13 Monoclonal Antibody (DE-K13)

Cytokeratin 10/13 (KRT10) Antibody – Mouse Monoclonal

Name: KRT10 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS438606
Price: 280 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS438606

MBS438606 is an antibody targeting KRT10. Also known as Cytokeratin 10/13 Mouse Monoclonal Antibody. Reactive in Human, Cat. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Cytokeratin 10/13 (KRT10), Monoclonal Antibody

Full Product Name

Cytokeratin 10/13 Mouse Monoclonal Antibody

Product Gene Name

anti-KRT10 antibody

Product Synonym Names

BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10, CK13, K13, keratin type I cytoskeletal 13, Keratin-13, Krt1-13, KRT1

Clonality

Monoclonal

Isotype

IgG2a, kappa

Clone Number

DE-K13

Host

Mouse

Species Reactivity

Human, Cat

Form/Format

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Specificity

This antibody recognizes cytokeratin 10 (56.5kDa) and cytokeratin 13 (53kDa) in Western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this MAb serves as differentiation-related marker of all stratified epithelia; it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas.

Preparation and Storage

Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Molecular Weight

56.5kDa (CK10); 53kDa (CK13)

OMIM

113800

3D Structure

ModBase 3D Structure for P13645

Cellular Localization

Cytoplasmic

Immunogen

Cytoskeletal preparation extracted from human ectocervical epithelium

Hu-Chromosome Location

17q21.2

Positive Control

A431, HeLa, MCF7 cells. Tonsil, esophageal or bladder carcinoma.

Applications Tested/Suitable for anti-KRT10 antibody

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin

Application Notes for anti-KRT10 antibody

Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Western Blot (0.25-0.5ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1.0ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Product References and Citations for anti-KRT10 antibody

Ivanyi D et. al. Journal of Pathology, 1989, 159:7-12.,Ivanyi, D., Minke, J. M., Hageman, C., Groeneveld, E., and van Doornewaard, G. (1992). Patterns of expression of feline cytokeratins in healthy epithelia and mammary carcinoma cells, Am J Vet Res 53, 304-14.,Ivanyi, D., Minke, J. M., Hageman, C., Groeneveld, E., van Doornewaard, G., and Misdorp, W. (1993). Cytokeratins as markers of initial stages of squamous metaplasia in feline mammary carcinomas, Am J Vet Res 54, 1095

Other Notes

Small volumes of anti-KRT10 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for KRT10. It may not necessarily be applicable to this product.

NCBI GI #

195972866

NCBI GeneID

3858

NCBI Accession #

NP_000412.3

UniProt Primary Accession #

P13645

UniProt Secondary Accession #

Q14664; Q8N175

UniProt Related Accession #

P13645

GenBank Nucleotide #

NM_000421.3

NCBI Official Full Name

keratin, type I cytoskeletal 10

NCBI Official Synonym Full Names

keratin 10

NCBI Official Symbol

KRT10

NCBI Official Synonym Symbols

BIE; EHK; K10; KPP; BCIE; CK10

UniProt Protein Name

Keratin, type I cytoskeletal 10

UniProt Synonym Protein Names

Cytokeratin-10; CK-10; Keratin-10; K10

Protein Family

Keratin

UniProt Gene Name

KRT10

UniProt Synonym Gene Names

KPP; CK-10; K10

UniProt Entry Name

K1C10_HUMAN

NCBI Protein Information

keratin, type I cytoskeletal 10

NCBI Summary for KRT10

This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. [provided by RefSeq, Jul 2008]

UniProt Comments for KRT10

K10: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K1.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: cytoplasm; extracellular space; membrane; nucleus

Biological Process: keratinocyte differentiation

Disease: Epidermolytic Hyperkeratosis; Erythroderma, Ichthyosiform, Congenital Reticular; Ichthyosis, Cyclic, With Epidermolytic Hyperkeratosis

Research Articles on KRT10

1. Complete structure of an epithelial keratin 1/keratin 10 dimer has been presented.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-KRT10 antibody

Reference Product	PubMed Publications
KRT1 antibody	>66 publications with KRT10 and KRT1
KRT2 antibody	>9 publications with KRT10 and KRT2

Pathways associated with anti-KRT10 antibody

Products by Pathway	Pathway Diagram
Staphylococcus Aureus Infection Pathway antibodies	Staphylococcus Aureus Infection Pathway Diagram
Staphylococcus Aureus Infection Pathway antibodies	Staphylococcus Aureus Infection Pathway Diagram

Diseases associated with anti-KRT10 antibody

Disease Name	Pubmed Publications
Congenital Abnormalities Antibodies	>56 publications with KRT10 and Congenital Abnormalities
Hyperkeratosis, Epidermolytic Antibodies	>46 publications with KRT10 and Hyperkeratosis, Epidermolytic
Carcinoma, Squamous Cell Antibodies	>18 publications with KRT10 and Carcinoma, Squamous Cell
Skin Neoplasms Antibodies	>12 publications with KRT10 and Skin Neoplasms
Inflammation Antibodies	>11 publications with KRT10 and Inflammation
Necrosis Antibodies	>6 publications with KRT10 and Necrosis
Breast Neoplasms Antibodies	>5 publications with KRT10 and Breast Neoplasms
Fibrosis Antibodies	>4 publications with KRT10 and Fibrosis
Hyperplasia Antibodies	>4 publications with KRT10 and Hyperplasia
Disease Models, Animal Antibodies	>4 publications with KRT10 and Disease Models, Animal

Organs/Tissues associated with anti-KRT10 antibody

Organ/Tissue Name	Pubmed Publications
Skin Antibodies	>124 publications with KRT10 and Skin
Mouth Antibodies	>16 publications with KRT10 and Mouth
Lung Antibodies	>6 publications with KRT10 and Lung
Vascular Antibodies	>3 publications with KRT10 and Vascular
Eye Antibodies	>2 publications with KRT10 and Eye
Embryonic Tissue Antibodies	>2 publications with KRT10 and Embryonic Tissue
Ovary Antibodies	>2 publications with KRT10 and Ovary
Esophagus Antibodies	>2 publications with KRT10 and Esophagus
Brain Antibodies	>1 publications with KRT10 and Brain
Heart Antibodies	>1 publications with KRT10 and Heart