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Ep-CAM /CD326 (EPCAM) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS438621

MBS438621 is an antibody targeting Epcam. Also known as Ep-CAM /CD326 (Rat) (Epithelial Marker) Mouse Monoclonal Antibody. Reactive in Rat, Mouse.. Host: Mouse. More details

$280 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Ep-CAM /CD326 (Epcam), Monoclonal Antibody
Full Product Name
Ep-CAM /CD326 (Rat) (Epithelial Marker) Mouse Monoclonal Antibody
Product Gene Name
anti-Epcam antibody
Product Synonym Names
Adenocarcinoma-associated Antigen; Cell Surface Glycoprotein Trop-1; EGP2; EGP314; EGP40; Epithelial Cell Adhesion Molecule; Epithelial Glycoprotein 314; ESA; KSA; TACD1; TACSTD1; TROP1; Tumor-associated Calcium Signal Transducer 1, ECS-1, Epidermal Surface Antigen 1, ESA1, FLOT2, Flotillin-2, M17S1 (Membrane Component, Chromosome 17, Surface Marker-1), REG-1, Reggie-1, Reggie-2
Clonality
Monoclonal
Isotype
IgG1/ Kappa
Clone Number
Epcam/1159
Host
Mouse
Species Reactivity
Rat, Mouse.
Form/Format
Bioreactor Concentrate with 0.05% Azide.
Specificity
Epitopes of Epcam/1158 and Epcam/1159 monoclonal antibodies are different. EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas.
Preparation and Storage
Store at 2 to 8 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
40-43kDa
3D Structure
ModBase 3D Structure for O55159
Cellular Localization
Cell Junction, Lateral cell membrane. Tight junction
Immunogen
Recombinant full-length Rat Epcam protein
Hu-Chromosome Location
6q12
Positive Control
Recombinant full-length rat Epcam protein
Applications Tested/Suitable for anti-Epcam antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin
Application Notes for anti-Epcam antibody
Immunohistochemistry (Formalin-fixed) (1:50-1:100 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunohistochemistry (IHC) of anti-Epcam antibody

Formalin-fixed, paraffin-embedded Rat Colon stained with Ep-CAM Monoclonal Antibody (Epcam/1159).
Mouse Anti-EPCAM Monoclonal Antibody – Cat# MBS438621

Immunohistochemistry (IHC) of anti-Epcam antibody

Formalin-fixed, paraffin-embedded Rat Lung stained with Ep-CAM Monoclonal Antibody (Epcam/1159).
Mouse Anti-EPCAM Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS438621

Product References and Citations for anti-Epcam antibody

Litvinov SV et. al. J Cell Biol, 1994, 125:437-46. 2. Tsubura A et. al. J Cut Pathol, 1992, 19:73-9

Other Notes

Small volumes of anti-Epcam antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Epcam. It may not necessarily be applicable to this product.

NCBI GI #
25742698
NCBI GeneID
171577
NCBI Accession #
NP_612550.1
UniProt Primary Accession #
O55159
UniProt Related Accession #
O55159
GenBank Nucleotide #
NM_138541.1
NCBI Official Full Name
epithelial cell adhesion molecule
NCBI Official Synonym Full Names
epithelial cell adhesion molecule
NCBI Official Symbol
Epcam
NCBI Official Synonym Symbols
Egp314; Tacstd1
UniProt Protein Name
Epithelial cell adhesion molecule
UniProt Synonym Protein Names
Epithelial glycoprotein 314; EGP314; Protein D5.7A; Tumor-associated calcium signal transducer 1; CD_antigen: CD326
Protein Family
Epithelial cell adhesion molecule
UniProt Gene Name
Epcam
UniProt Synonym Gene Names
Tacstd1; Ep-CAM; EGP314
UniProt Entry Name
EPCAM_RAT
NCBI Protein Information
epithelial cell adhesion molecule

NCBI Summary for Epcam

involved in metastasis formation and tumor progression [RGD, Feb 2006]

UniProt Comments for Epcam

EPCAM: a single-pass type I membrane protein of the EPCAM family. May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinomas including pancreatic carcinoma. May be a marker of cancer stem cells.

Protein type: Cell adhesion; Membrane protein, integral; Cell surface; Cell cycle regulation; Motility/polarity/chemotaxis

Cellular Component: apical plasma membrane; basolateral plasma membrane; cell surface; integral to membrane; integral to plasma membrane; lateral plasma membrane; plasma membrane; tight junction

Molecular Function: protein complex binding

Biological Process: negative regulation of apoptosis; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; stem cell differentiation
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Research Articles on Epcam

1. These results suggest that, prior to implantation, EpCAM mediates intercellular adhesion in the uterine epithelium.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Epcam antibody

 Reference Product  PubMed Publications
 Myc antibody  >64 publications with Epcam and Myc
 Prom1 antibody  >58 publications with Epcam and Prom1
 Ptprc antibody  >26 publications with Epcam and Ptprc
 Tp53 antibody  >22 publications with Epcam and Tp53
 Cldn7 antibody  >20 publications with Epcam and Cldn7
 Tspan8 antibody  >8 publications with Epcam and Tspan8
 Ccnd1 antibody  >7 publications with Epcam and Ccnd1
 Nfkb1 antibody  >2 publications with Epcam and Nfkb1
 Gpnmb antibody  >1 publications with Epcam and Gpnmb

Diseases associated with anti-Epcam antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >733 publications with Epcam and Carcinoma
 Neoplasm Metastasis Antibodies  >456 publications with Epcam and Neoplasm Metastasis
 Adenocarcinoma Antibodies  >322 publications with Epcam and Adenocarcinoma
 Breast Neoplasms Antibodies  >260 publications with Epcam and Breast Neoplasms
 Liver Diseases Antibodies  >223 publications with Epcam and Liver Diseases
 Liver Neoplasms Antibodies  >212 publications with Epcam and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >198 publications with Epcam and Carcinoma, Hepatocellular
 Lung Neoplasms Antibodies  >140 publications with Epcam and Lung Neoplasms
 Neoplasms, Experimental Antibodies  >139 publications with Epcam and Neoplasms, Experimental
 Ovarian Neoplasms Antibodies  >109 publications with Epcam and Ovarian Neoplasms

Organs/Tissues associated with anti-Epcam antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >221 publications with Epcam and Lung
 Colon Antibodies  >180 publications with Epcam and Colon
 Prostate Antibodies  >121 publications with Epcam and Prostate
 Pancreas Antibodies  >47 publications with Epcam and Pancreas
 Kidney Antibodies  >47 publications with Epcam and Kidney
 Thymus Antibodies  >24 publications with Epcam and Thymus
 Spleen Antibodies  >16 publications with Epcam and Spleen
 Mammary Gland Antibodies  >14 publications with Epcam and Mammary Gland
 Testis Antibodies  >14 publications with Epcam and Testis
 Placenta Antibodies  >6 publications with Epcam and Placenta
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