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Topoisomerase (DNA) I, Mitochondrial (TOP1MT) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS438648

MBS438648 is an antibody targeting TOP1MT. Also known as Topoisomerase (DNA) I, Mitochondrial (TOP1MT) Mouse Monoclonal Antibody. Reactive in Human. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Topoisomerase (DNA) I, Mitochondrial (TOP1MT), Monoclonal Antibody
Full Product Name
Topoisomerase (DNA) I, Mitochondrial (TOP1MT) Mouse Monoclonal Antibody
Product Gene Name
anti-TOP1MT antibody
Product Synonym Names
DNA topoisomerase I; TOP1MT; Topoisomerase I mitochondrial; Type IB Topoisomerase
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
TOP1MT/488
Host
Mouse
Species Reactivity
Human
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
70kDa
OMIM
606387
3D Structure
ModBase 3D Structure for Q969P6
Cellular Localization
Cytoplasmic (Mitochondria)
Immunogen
Recombinant full-length human TOP1MT protein
Hu-Chromosome Location
8q24.3
Positive Control
A431 cells. Heart, Skeletal muscle, brain or fetal liver.
Applications Tested/Suitable for anti-TOP1MT antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
Application Notes for anti-TOP1MT antibody
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Immunohistochemistry (IHC) of anti-TOP1MT antibody

Formalin-fixed, paraffin-embedded human Gallbladder stained with Topo I, MT Monoclonal Antibody (TOP1MT/488).
Mouse Anti-TOP1MT Monoclonal Antibody – Cat# MBS438648

Immunohistochemistry (IHC) of anti-TOP1MT antibody

Formalin-fixed, paraffin-embedded human Melanoma stained with Topo I, MT Monoclonal Antibody (TOP1MT/488).
Mouse Anti-TOP1MT Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS438648

Immunohistochemistry (IHC) of anti-TOP1MT antibody

Formalin-fixed, paraffin-embedded human Pancreas stained with Topo I, MT Monoclonal Antibody (TOP1MT/488).
Mouse Anti-TOP1MT Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS438648

Product References and Citations for anti-TOP1MT antibody

Zhang, H., Barcel-, J.M., Lee, B., Kohlhagen, G., Zimonjic, D.B., Popescu, N.C. and Pommier, Y. 2001. Human mitochondrial topoisomerase I. Proc. Natl. Acad. Sci. USA 98: 10608-10613. Zhang, H., Meng, L.H. and Pommier, Y. 2007. Mitochondrial topoisomerases and alternative splicing of the human TOP1mt gene. Biochimie 89: 474-481

Other Notes

Small volumes of anti-TOP1MT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TOP1MT. It may not necessarily be applicable to this product.

NCBI GI #
386642871
NCBI GeneID
116447
NCBI Accession #
NP_001245375.1
UniProt Primary Accession #
Q969P6
UniProt Secondary Accession #
Q86ST4; Q86V82; B7ZAR5; E7ES89
UniProt Related Accession #
Q969P6
GenBank Nucleotide #
NM_001258446.1
NCBI Official Full Name
DNA topoisomerase I, mitochondrial isoform 2
NCBI Official Synonym Full Names
topoisomerase (DNA) I, mitochondrial
NCBI Official Symbol
TOP1MT
UniProt Protein Name
DNA topoisomerase I, mitochondrial
Protein Family
DNA topoisomerase
UniProt Gene Name
TOP1MT
UniProt Synonym Gene Names
TOP1mt
UniProt Entry Name
TOP1M_HUMAN
NCBI Protein Information
DNA topoisomerase I, mitochondrial

NCBI Summary for TOP1MT

This gene encodes a mitochondrial DNA topoisomerase that plays a role in the modification of DNA topology. The encoded protein is a type IB topoisomerase and catalyzes the transient breaking and rejoining of DNA to relieve tension and DNA supercoiling generated in the mitochondrial genome during replication and transcription. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]
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UniProt Comments for TOP1MT

TOP1MT: Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Belongs to the type IB topoisomerase family.

Protein type: Topoisomerase; Mitochondrial; EC 5.99.1.2

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: mitochondrion; nucleolus; nucleus

Molecular Function: DNA topoisomerase type I activity

Biological Process: chromatin remodeling; chromosome segregation; DNA replication; DNA topological change
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Research Articles on TOP1MT

1. Data indicate that the different coordination geometry achieved by the two transition metal ions has an important role in modulating their efficiency as topoisomerase I inhibitors.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TOP1MT antibody

 Reference Product  PubMed Publications
 TDP1 antibody  >2 publications with TOP1MT and TDP1
 MUS81 antibody  >1 publications with TOP1MT and MUS81

Diseases associated with anti-TOP1MT antibody

 Disease Name  Pubmed Publications
 Poisoning Antibodies  >2 publications with TOP1MT and Poisoning
 Drug-Related Side Effects and Adverse Reactions Antibodies  >2 publications with TOP1MT and Drug-Related Side Effects and Adverse Reactions
 Premature Birth Antibodies  >1 publications with TOP1MT and Premature Birth
 Blood Coagulation Disorders Antibodies  >1 publications with TOP1MT and Blood Coagulation Disorders
 Drug Hypersensitivity Antibodies  >1 publications with TOP1MT and Drug Hypersensitivity
 Drug-Induced Liver Injury Antibodies  >1 publications with TOP1MT and Drug-Induced Liver Injury

Organs/Tissues associated with anti-TOP1MT antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >3 publications with TOP1MT and Blood
 Lung Antibodies  >3 publications with TOP1MT and Lung
 Liver Antibodies  >1 publications with TOP1MT and Liver
 Intestine Antibodies  >1 publications with TOP1MT and Intestine
 Prostate Antibodies  >1 publications with TOP1MT and Prostate
 Vascular Antibodies  >1 publications with TOP1MT and Vascular
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