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ACTA1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS438670

MBS438670 is an antibody targeting ACTA1. Also known as Actin, Muscle Specific (Muscle Cell Marker) Mouse Monoclonal Antibody. Reactive in Human, Rabbit, Rat. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Actin (ACTA1), Monoclonal Antibody
Full Product Name
Actin, Muscle Specific (Muscle Cell Marker) Mouse Monoclonal Antibody
Product Gene Name
anti-ACTA1 antibody
Product Synonym Gene Name
ACTA2; ACTG2
Product Synonym Names
ACTA, ACTA1, ACTA2, ACTC1, Actin, ACTSA, Alpha-2 actin, alpha skeletal muscle, Alpha-actin-1, Cardiac muscle alpha actin-1, Skeletal muscle alpha actin-1
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
MSA/953
Host
Mouse
Species Reactivity
Human, Rabbit, Rat
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
42,009 Da
OMIM
102620
3D Structure
ModBase 3D Structure for P62736
Cellular Localization
Cytoplasmic
Immunogen
Synthetic peptide common among human muscle actins
Hu-Chromosome Location
1q42.13 (ACTA1); 10q23.3 (ACTA2); 2p13.1 (ACTG2)
Positive Control
Muscle or sarcoma
Applications Tested/Suitable for anti-ACTA1 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin
Application Notes for anti-ACTA1 antibody
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Western Blot (0.5-1.0ug/ml)
Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Immunohistochemistry (IHC) of anti-ACTA1 antibody

Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with Muscle Specific Actin Monoclonal Antibody (MSA/953)
Mouse Anti-ACTA1 Monoclonal Antibody – Cat# MBS438670

Immunohistochemistry (IHC) of anti-ACTA1 antibody

Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with Muscle Specific Actin Monoclonal Antibody (MSA/953)
Mouse Anti-ACTA1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS438670

Western Blot (WB) of anti-ACTA1 antibody

Western Blot of hSKM Cell Lysate using Muscle Specific Actin Monoclonal Antibody (MSA/953)
Mouse Anti-ACTA1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS438670

Immunohistochemistry (IHC) of anti-ACTA1 antibody

Formalin-fixed, paraffin-embedded Rat Lung stained with Muscle Specific Actin Monoclonal Antibody (MSA/953)
Mouse Anti-ACTA1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS438670

Immunohistochemistry (IHC) of anti-ACTA1 antibody

Formalin-fixed, paraffin-embedded Rat Heart stained with Muscle Specific Actin Monoclonal Antibody (MSA/953)
Mouse Anti-ACTA1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS438670

Immunohistochemistry (IHC) of anti-ACTA1 antibody

Formalin-fixed, paraffin-embedded Rat Stomach stained with Muscle Specific Actin Monoclonal Antibody (MSA/953)
Mouse Anti-ACTA1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS438670

Immunohistochemistry (IHC) of anti-ACTA1 antibody

Formalin-fixed, paraffin-embedded Rat Skeletal Muscle stained with Muscle Specific Actin Monoclonal Antibody (MSA/953)
Mouse Anti-ACTA1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS438670

Product References and Citations for anti-ACTA1 antibody

Barkalow, K. and Hartwig, J.H. 1995. Actin cytoskeleton. Setting the pace of cell movement. Curr. Biol. 5: 1000-1002.

Other Notes

Small volumes of anti-ACTA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ACTA1. It may not necessarily be applicable to this product.

NCBI GI #
213688375
NCBI GeneID
59
NCBI Accession #
NP_001135417.1
UniProt Primary Accession #
P62736
UniProt Secondary Accession #
P03996; P04108; Q6FI19; B2R8A4
UniProt Related Accession #
P62736
GenBank Nucleotide #
NM_001141945.2
NCBI Official Full Name
actin, aortic smooth muscle
NCBI Official Synonym Full Names
actin, alpha 2, smooth muscle, aorta
NCBI Official Symbol
ACTA2
NCBI Official Synonym Symbols
AAT6; ACTSA; MYMY5
UniProt Protein Name
Actin, aortic smooth muscle
UniProt Synonym Protein Names
Alpha-actin-2; Cell growth-inhibiting gene 46 protein
Protein Family
Actin
UniProt Gene Name
ACTA2
UniProt Synonym Gene Names
ACTSA; ACTVS
UniProt Entry Name
ACTA_HUMAN
NCBI Protein Information
actin, aortic smooth muscle

NCBI Summary for ACTA1

The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]
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UniProt Comments for ACTA1

ACTA2: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Up-regulated in response to enterovirus 71 (EV71) infection. Belongs to the actin family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis; Contractile

Chromosomal Location of Human Ortholog: 10q23.3

Cellular Component: cytoplasm; cytosol; extracellular space; filopodium; lamellipodium; protein complex

Molecular Function: protein kinase binding

Biological Process: muscle contraction; response to virus

Disease: Aortic Aneurysm, Familial Thoracic 6; Moyamoya Disease 5; Multisystemic Smooth Muscle Dysfunction Syndrome
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Research Articles on ACTA1

1. NGF has a role in modulating trkANGFR/p75NTR in alphaSMA-expressing conjunctival fibroblasts from human ocular cicatricial pemphigoid

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ACTA1 antibody

 Reference Product  PubMed Publications
 MYH11 antibody  >50 publications with ACTA1 and MYH11
 SRF antibody  >20 publications with ACTA1 and SRF
 MYOCD antibody  >10 publications with ACTA1 and MYOCD
 TPM3 antibody  >8 publications with ACTA1 and TPM3
 TPM1 antibody  >2 publications with ACTA1 and TPM1
 UBC antibody  >2 publications with ACTA1 and UBC
 TPM2 antibody  >1 publications with ACTA1 and TPM2

Pathways associated with anti-ACTA1 antibody

 Products by Pathway  Pathway Diagram
 Muscle Contraction Pathway antibodies  Muscle Contraction Pathway Diagram
 Myometrial Relaxation And Contraction Pathways antibodies  Myometrial Relaxation And Contraction Pathways Diagram
 Smooth Muscle Contraction Pathway antibodies  Smooth Muscle Contraction Pathway Diagram
 Striated Muscle Contraction Pathway antibodies  Striated Muscle Contraction Pathway Diagram
 Vascular Smooth Muscle Contraction Pathway antibodies  Vascular Smooth Muscle Contraction Pathway Diagram
 Vascular Smooth Muscle Contraction Pathway antibodies  Vascular Smooth Muscle Contraction Pathway Diagram

Diseases associated with anti-ACTA1 antibody

 Disease Name  Pubmed Publications
 Fibrosis Antibodies  >348 publications with ACTA1 and Fibrosis
 Cardiovascular Diseases Antibodies  >155 publications with ACTA1 and Cardiovascular Diseases
 Inflammation Antibodies  >114 publications with ACTA1 and Inflammation
 Liver Diseases Antibodies  >105 publications with ACTA1 and Liver Diseases
 Disease Models, Animal Antibodies  >88 publications with ACTA1 and Disease Models, Animal
 Liver Cirrhosis Antibodies  >75 publications with ACTA1 and Liver Cirrhosis
 Kidney Diseases Antibodies  >57 publications with ACTA1 and Kidney Diseases
 Heart Diseases Antibodies  >49 publications with ACTA1 and Heart Diseases
 Aortic Aneurysm, Thoracic Antibodies  >45 publications with ACTA1 and Aortic Aneurysm, Thoracic
 Breast Neoplasms Antibodies  >40 publications with ACTA1 and Breast Neoplasms

Organs/Tissues associated with anti-ACTA1 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >840 publications with ACTA1 and Muscle
 Vascular Antibodies  >259 publications with ACTA1 and Vascular
 Blood Antibodies  >191 publications with ACTA1 and Blood
 Lung Antibodies  >150 publications with ACTA1 and Lung
 Eye Antibodies  >141 publications with ACTA1 and Eye
 Liver Antibodies  >134 publications with ACTA1 and Liver
 Connective Tissue Antibodies  >131 publications with ACTA1 and Connective Tissue
 Kidney Antibodies  >104 publications with ACTA1 and Kidney
 Skin Antibodies  >88 publications with ACTA1 and Skin
 Heart Antibodies  >70 publications with ACTA1 and Heart
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