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Beta-2 Microglobulin (B2M) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS438711

MBS438711 is an antibody targeting B2M. Also known as Beta-2 Microglobulin (Renal Failure & Tumor Marker) Mouse Monoclonal Antibody. Reactive in Human, Non-Human Primates. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Beta-2 Microglobulin (B2M), Monoclonal Antibody
Full Product Name
Beta-2 Microglobulin (Renal Failure & Tumor Marker) Mouse Monoclonal Antibody
Product Gene Name
anti-B2M antibody
Product Synonym Names
B2M; Beta 2 microglobin; Beta 2 microglobulin; Beta chain of MHC class I molecules; Beta-2-microglobulin form pI 5.3; Hdcma22p
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
B2M/1118
Host
Mouse
Species Reactivity
Human, Non-Human Primates
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
beta 2 microglobulin is a 12KDa protein with a pI of 5.6. Serum beta 2 microglobulin levels are a reflection of cell turnover. Levels rise with fever, inflammation, and infection. Increased serum levels are also seen in B-cell malignancies and in renal failure and may indicate a worse prognosis for patients with early-stage Hodgkin's lymphoma. In urine, increased levels are seen in proximal renal tubular disease as well as renal transplant rejection. beta 2 microglobulin levels can rise either because its rate of synthesis has increased (e.g. in AIDS, malignant monoclonal plasma cell dyscrasia, solid tumours and autoimmune disease) or because of impaired renal filtration (e.g. due to renal insufficiency, graft rejection or nephrotoxicity induced by post-transplantation immunosuppressive therapy).
Preparation and Storage
Antibody with azide - store at 2 to 8°C. Non-hazardous. No MSDS Required.
Molecular Weight
12kDa
OMIM
109700
3D Structure
ModBase 3D Structure for P61769
Cellular Localization
Cell Surface, Secreted
Immunogen
Full length recombinant human B2M protein
Positive Control
HL-60 or HeLa cells. Melanomas and Lymphoma. Carcinoma of Stomach, Cervix, Endometrial, Kidney or Colon.
Applications Tested/Suitable for anti-B2M antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-B2M antibody
Flow Cytometry: 1-2ug/million cells
Immunofluorescence: 1-3ug/ml
Immunohistochemistry: 1-2ug/ml (30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Western Blot: 2-4ug/ml

Immunohistochemistry (IHC) of anti-B2M antibody

Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with Beta-2-Microglobulin Monoclonal Antibody (B2M/1118)
Mouse Anti-B2M Monoclonal Antibody – Cat# MBS438711

Immunohistochemistry (IHC) of anti-B2M antibody

Formalin-fixed, paraffin-embedded human Endometrial Carcinoma stained with Beta-2-Microglobulin Monoclonal Antibody (B2M/1118)
Mouse Anti-B2M Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS438711

Immunohistochemistry (IHC) of anti-B2M antibody

Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with Beta-2-Microglobulin Monoclonal Antibody (B2M/1118)
Mouse Anti-B2M Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS438711

Immunofluorescence (IF) of anti-B2M antibody

Immunofluorescent staining of HeLa cells. Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/1118); followed by goat anti-mouse IgG-CF488 (Green).
Mouse Anti-B2M Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS438711

Testing Data of anti-B2M antibody

Purified Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/1118). Confirmation of Integrity and Purity of Antibody.
Mouse Anti-B2M Monoclonal Antibody, Testing Data – Cat# MBS438711

Flow Cytometric (FC) of anti-B2M antibody

Flow Cytometric Analysis of HeLa cells using Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/1118); followed bygoat anti-mouse IgG-CF488 (Blue); Unstained cells Control (Red).
Mouse Anti-B2M Monoclonal Antibody, Flow Cytometric (FC) – Cat# MBS438711

Western Blot (WB) of anti-B2M antibody

Western Blot Analysis of THP-1 and Raji Cell lysate using Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/1118).
Mouse Anti-B2M Monoclonal Antibody, Western Blot (WB) – Cat# MBS438711

Product References and Citations for anti-B2M antibody

Liabeuf A, le Borgne de Kaouel C, Kourilsky FM, Malissen B, Manuel Y, Sanderson AR. An antigenic determinant of human beta 2-microglobulin masked by the association with HLA heavy chains at the cell surface: analysis using monoclonal antibodies. J Immunol. 1981 Oct;127(4):1542-8.

Other Notes

Small volumes of anti-B2M antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for B2M. It may not necessarily be applicable to this product.

NCBI GI #
4757826
NCBI GeneID
567
NCBI Accession #
NP_004039.1
UniProt Primary Accession #
P61769
UniProt Secondary Accession #
P01884; Q540F8; Q6IAT8; Q9UCK0; Q9UD48; Q9UDF4
UniProt Related Accession #
P61769
GenBank Nucleotide #
NM_004048.2
NCBI Official Full Name
beta-2-microglobulin
NCBI Official Synonym Full Names
beta-2-microglobulin
NCBI Official Symbol
B2M
NCBI Official Synonym Symbols
IMD43
UniProt Protein Name
Beta-2-microglobulin
Protein Family
Beta-2-microglobulin
UniProt Gene Name
B2M
UniProt Entry Name
B2MG_HUMAN
NCBI Protein Information
beta-2-microglobulin

NCBI Summary for B2M

This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.[provided by RefSeq, Aug 2014]
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UniProt Comments for B2M

B2M: Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Heterodimer of an alpha chain and a beta chain. Beta-2- microglobulin is the beta-chain of major histocompatibility complex class I molecules. Polymers of beta 2-microglobulin can be found in tissues from patients on long-term hemodialysis. Belongs to the beta-2-microglobulin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 15q21.1

Cellular Component: cytoplasm; early endosome membrane; endoplasmic reticulum lumen; extracellular region; extracellular space; focal adhesion; Golgi apparatus; Golgi membrane; membrane; phagocytic vesicle membrane; plasma membrane

Molecular Function: glycoprotein binding; identical protein binding; protein binding

Biological Process: antibacterial humoral response; antigen processing and presentation of endogenous peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; antigen processing and presentation of peptide antigen via MHC class I; cellular protein metabolic process; defense response to Gram-negative bacterium; defense response to Gram-positive bacterium; innate immune response; iron ion homeostasis; positive regulation of protein binding; positive regulation of receptor-mediated endocytosis; positive regulation of T cell cytokine production; regulation of defense response to virus by virus; regulation of immune response; retinal homeostasis

Disease: Hypoproteinemia, Hypercatabolic
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Research Articles on B2M

1. beta2M-mediated VEGFR-2/Akt/mTOR phosphorylation and tumor angiogenesis was significantly suppressed by over-expression of DKK-3.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-B2M antibody

 Reference Product  PubMed Publications
 JUN antibody  >85 publications with B2M and JUN
 HLA-A antibody  >16 publications with B2M and HLA-A
 HLA-B antibody  >12 publications with B2M and HLA-B
 HLA-C antibody  >4 publications with B2M and HLA-C
 FCGRT antibody  >3 publications with B2M and FCGRT
 HFE antibody  >3 publications with B2M and HFE
 CALR antibody  >1 publications with B2M and CALR

Pathways associated with anti-B2M antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Amyloid Fiber Formation Pathway antibodies  Amyloid Fiber Formation Pathway Diagram
 Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway antibodies  Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Antigen Processing-Cross Presentation Pathway antibodies  Antigen Processing-Cross Presentation Pathway Diagram
 Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies  Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram

Diseases associated with anti-B2M antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >92 publications with B2M and Cardiovascular Diseases
 Kidney Diseases Antibodies  >78 publications with B2M and Kidney Diseases
 Carcinoma Antibodies  >44 publications with B2M and Carcinoma
 Inflammation Antibodies  >44 publications with B2M and Inflammation
 Renal Insufficiency Antibodies  >33 publications with B2M and Renal Insufficiency
 Disease Models, Animal Antibodies  >30 publications with B2M and Disease Models, Animal
 Proteinuria Antibodies  >25 publications with B2M and Proteinuria
 Necrosis Antibodies  >24 publications with B2M and Necrosis
 Adenocarcinoma Antibodies  >22 publications with B2M and Adenocarcinoma
 Liver Diseases Antibodies  >22 publications with B2M and Liver Diseases

Organs/Tissues associated with anti-B2M antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >311 publications with B2M and Blood
 Kidney Antibodies  >122 publications with B2M and Kidney
 Bone Antibodies  >90 publications with B2M and Bone
 Bone Marrow Antibodies  >68 publications with B2M and Bone Marrow
 Liver Antibodies  >45 publications with B2M and Liver
 Muscle Antibodies  >36 publications with B2M and Muscle
 Brain Antibodies  >35 publications with B2M and Brain
 Heart Antibodies  >32 publications with B2M and Heart
 Skin Antibodies  >32 publications with B2M and Skin
 Vascular Antibodies  >26 publications with B2M and Vascular
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