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CD28 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS438728

MBS438728 is an antibody targeting CD28. Also known as CD28 Mouse Monoclonal Antibody. Reactive in Human, Pig, Cow, Sheep, Mouse. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD28, Monoclonal Antibody
Full Product Name
CD28 Mouse Monoclonal Antibody
Product Gene Name
anti-CD28 antibody
Product Synonym Names
T-cell-specific surface glycoprotein CD28; Tp44
Clonality
Monoclonal
Isotype
IgG2a, kappa
Clone Number
204-12
Host
Mouse
Species Reactivity
Human, Pig, Cow, Sheep, Mouse
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
Recognizes a glycoprotein of 44-88kDa, which is identified as CD28. It is the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing T-cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.
Preparation and Storage
Antibody without azide - store at -20 to -80 degree C. Non-hazardous. No MSDS required.
Molecular Weight
44kDa (monomer); 88kDa (dimer)
OMIM
186760
3D Structure
ModBase 3D Structure for P10747
Cellular Localization
Cell surface, Membrane
Immunogen
Stimulated human leucocytes
Positive Control
Jurkat cells. Human lymph nodes and tonsils.
Research Areas
AKT Signaling, Cancer, Cardiovascular, Dendritic Cell Marker, Hematopoietic Stem Cells, Immune checkpoint, Immuno Oncology, Immunology, Infectious Disease, Signal Transduction
Applications Tested/Suitable for anti-CD28 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes for anti-CD28 antibody
Flow Cytometry (Flow); Tested Dillution: 1-2ug/million cells; Protocol: Flow Cytometry Protocol
Immunofluorescence (IF); Tested Dillution: 1-3ug/ml

Flow Cytometry of anti-CD28 antibody

Flow Cytometric Analysis of paraformaldehyde-fixed Jurkat cells using CD28 Mouse Monoclonal Antibody (MBS438728) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
Mouse Anti-CD28 Monoclonal Antibody – Cat# MBS438728

SDS-PAGE Analysis of anti-CD28 antibody

SDS-PAGE Analysis of Purified CD28 Mouse Monoclonal Antibody (MBS438728). Confirmation of Integrity and Purity of Antibody.
Mouse Anti-CD28 Monoclonal Antibody, SDS-PAGE Analysis – Cat# MBS438728

Immunofluorescence of anti-CD28 antibody

Immunofluorescent staining of Jurkat cells using CD28 Mouse Monoclonal Antibody (MBS438728) followed by goat anti-Mouse IgG conjugated to CF488 (green). Red Dot is used to labelnuclei (red).
Mouse Anti-CD28 Monoclonal Antibody, Immunofluorescence – Cat# MBS438728

Product References and Citations for anti-CD28 antibody

In Leucocyte typing Workshop VI. Sandilands, G. et al. Clinical and Experimental Immunology, 162(3), 516-27 (2010)

Other Notes

Small volumes of anti-CD28 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD28. It may not necessarily be applicable to this product.

NCBI GI #
340545508
NCBI GeneID
940
NCBI Accession #
NP_001230006.1
UniProt Primary Accession #
P10747
UniProt Secondary Accession #
Q13964; Q52M23; Q70WG0; Q8NI54; Q8NI55; Q8NI56; Q8WXJ2; Q9BYV0; A8KAC1
UniProt Related Accession #
P10747
GenBank Nucleotide #
NM_001243077.1
NCBI Official Full Name
T-cell-specific surface glycoprotein CD28 isoform 2
NCBI Official Synonym Full Names
CD28 molecule
NCBI Official Symbol
CD28
NCBI Official Synonym Symbols
Tp44
UniProt Protein Name
T-cell-specific surface glycoprotein CD28
UniProt Synonym Protein Names
TP44; CD_antigen: CD28
Protein Family
T-cell-specific surface glycoprotein
UniProt Gene Name
CD28
UniProt Entry Name
CD28_HUMAN
NCBI Protein Information
T-cell-specific surface glycoprotein CD28

NCBI Summary for CD28

The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]

UniProt Comments for CD28

CD28: a type I plasma membrane protein Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Binds to B7-1 and -2. Expressed in T cells and plasma cells, but not in less mature B cells. Six splice-variant isoforms have been described.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q33

Cellular Component: cell surface; cytosol; external side of plasma membrane; integral to plasma membrane; plasma membrane

Molecular Function: coreceptor activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protease binding; protein binding; SH3/SH2 adaptor activity

Biological Process: cell surface receptor linked signal transduction; cytokine biosynthetic process; humoral immune response; phosphoinositide-mediated signaling; positive regulation of interleukin-10 production; positive regulation of interleukin-2 biosynthetic process; positive regulation of interleukin-4 production; positive regulation of mitosis; positive regulation of T cell proliferation; regulation of defense response to virus by virus; regulation of phosphoinositide 3-kinase cascade; regulatory T cell differentiation; T cell costimulation
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Research Articles on CD28

1. in complex with T cell receptor, promotes glycolysis

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD28 antibody

 Reference Product  PubMed Publications
 CD80 antibody  >1886 publications with CD28 and CD80
 CD86 antibody  >1140 publications with CD28 and CD86
 LCK antibody  >180 publications with CD28 and LCK
 VAV1 antibody  >64 publications with CD28 and VAV1
 ITK antibody  >48 publications with CD28 and ITK
 GRB2 antibody  >31 publications with CD28 and GRB2
 GRAP2 antibody  >7 publications with CD28 and GRAP2
 CD247 antibody  >1 publications with CD28 and CD247

Pathways associated with anti-CD28 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 CD28 Co-stimulation Pathway antibodies  CD28 Co-stimulation Pathway Diagram
 CD28 Dependent PI3K/Akt Signaling Pathway antibodies  CD28 Dependent PI3K/Akt Signaling Pathway Diagram
 CD28 Dependent Vav1 Pathway antibodies  CD28 Dependent Vav1 Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram

Diseases associated with anti-CD28 antibody

 Disease Name  Pubmed Publications
 Autoimmune Diseases Antibodies  >1314 publications with CD28 and Autoimmune Diseases
 Inflammation Antibodies  >904 publications with CD28 and Inflammation
 Necrosis Antibodies  >893 publications with CD28 and Necrosis
 HIV Infections Antibodies  >469 publications with CD28 and HIV Infections
 Arthritis, Rheumatoid Antibodies  >386 publications with CD28 and Arthritis, Rheumatoid
 Lung Diseases Antibodies  >345 publications with CD28 and Lung Diseases
 Cardiovascular Diseases Antibodies  >341 publications with CD28 and Cardiovascular Diseases
 Vascular Diseases Antibodies  >310 publications with CD28 and Vascular Diseases
 Lymphoma Antibodies  >310 publications with CD28 and Lymphoma
 Carcinoma Antibodies  >259 publications with CD28 and Carcinoma

Organs/Tissues associated with anti-CD28 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >8571 publications with CD28 and Blood
 Thymus Antibodies  >354 publications with CD28 and Thymus
 Brain Antibodies  >137 publications with CD28 and Brain
 Embryonic Tissue Antibodies  >56 publications with CD28 and Embryonic Tissue
 Pharynx Antibodies  >36 publications with CD28 and Pharynx
 Connective Tissue Antibodies  >29 publications with CD28 and Connective Tissue
 Placenta Antibodies  >21 publications with CD28 and Placenta
 Uterus Antibodies  >19 publications with CD28 and Uterus
 Trachea Antibodies  >11 publications with CD28 and Trachea
 Adipose Tissue Antibodies  >10 publications with CD28 and Adipose Tissue
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