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CD19 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS438777

MBS438777 is an antibody targeting CD19. Also known as CD19 (B-Lymphocyte Marker) Mouse Monoclonal Antibody. Reactive in Human, Monkey. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CD19, Monoclonal Antibody
Full Product Name
CD19 (B-Lymphocyte Marker) Mouse Monoclonal Antibody
Product Gene Name
anti-CD19 antibody
Product Synonym Names
B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; CVID3; Leu-12; Lymphocyte Surface Antigen; T-cell surface antigen Leu-12
Clonality
Monoclonal
Isotype
IgG1 / Kappa
Clone Number
CVID3/155
Host
Mouse
Species Reactivity
Human, Monkey
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
Recognizes a protein of 95kDa, identified as CD19. CD19 is a transmembrane glycoprotein that contains two extracellular immunoglobulin-like domains. CD19 is present in both benign and malignant B-cells and is considered to be the most reliable surface marker of this lineage over a wide range of maturational stages. In normal lymphoid tissue, CD19 is observed in germinal centers, in mantle zone cells, and in scattered cells of the inter-follicular areas. Anti-CD19 exhibits an overall immunoreactivity pattern similar to those of the antibodies against CD20 and CD22. However, in contrast to CD20, expression of CD19 is continuous throughout B-cell development and through terminal differentiation of B-cells into plasma cells. Anti-CD19 positivity is seen in the vast majority of B-cell neoplasms commonly at a lower intensity than normal B-cell counterparts. Plasma cell neoplasms are nearly always negative, as are T-cell neoplasms.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
95kDa
OMIM
107265
3D Structure
ModBase 3D Structure for P15391
Immunogen
Recombinant full-length human CD19 protein
Cellular Localization
Cell membrane, Membrane raft
Positive Control
hPBL or Raji cells. Human tonsil, lymph node or spleen.
Applications Tested/Suitable for anti-CD19 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes for anti-CD19 antibody
Flow Cytometry (Flow): 1-2ug/million cells

Immunofluorescence (IF): 1-3ug/ml

*Optimal dilution for a specific application should be determined.

Flow Cytometry (FC) of anti-CD19 antibody

Flow Cytometric Analysis of Raji cells. CD19 Mouse Monoclonal Antibody (CVID3/155) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Mouse Anti-CD19 Monoclonal Antibody – Cat# MBS438777

SDS-PAGE (SDS) of anti-CD19 antibody

SDS-PAGE Analysis of Purified CD19 Mouse Monoclonal Antibody (CVID3/155). Confirmation of Integrity and Purity of Antibody.
Mouse Anti-CD19 Monoclonal Antibody, SDS-PAGE (SDS) – Cat# MBS438777

Flow Cytometry (FC) of anti-CD19 antibody

Flow cytometric analysis of lymphocyte-gated PBMCs. CD19 Mouse Monoclonal Antibody (CVID3/155) labeled with CF568 (orange); unstained cells (gray).
Mouse Anti-CD19 Monoclonal Antibody, Flow Cytometry (FC) – Cat# MBS438777

Immunofluorescence (IF) of anti-CD19 antibody

Immunofluorescence staining of Raji cells. CD19 Mouse Monoclonal Antibody (CVID3/155) followed by goat anti-mouse IgG-CF488. Nuclear counterstain is RedDot
Mouse Anti-CD19 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS438777

Product References and Citations for anti-CD19 antibody

Reinherz EL, Haynes BF, Nadler LM, Berstein ID, ed. Leukocyte Typing I Human B Lymphocytes. New York: Springer-Verlag New York Inc; 1986:3-560. Knap W, Dorken B,Riebr EP, et. al., ed. Leucoyte Typing IV. New York: Oxford University Pres; 198.(Biology

Other Notes

Small volumes of anti-CD19 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD19. It may not necessarily be applicable to this product.

NCBI GI #
296010921
NCBI GeneID
930
NCBI Accession #
NP_001171569.1
UniProt Primary Accession #
P15391
UniProt Secondary Accession #
Q96S68; Q9BRD6; A0N0P9; F5H635
UniProt Related Accession #
P15391
GenBank Nucleotide #
NM_001178098.1
NCBI Official Full Name
B-lymphocyte antigen CD19 isoform 1
NCBI Official Synonym Full Names
CD19 molecule
NCBI Official Symbol
CD19
NCBI Official Synonym Symbols
B4; CVID3
UniProt Protein Name
B-lymphocyte antigen CD19
UniProt Synonym Protein Names
B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12; CD_antigen: CD19
Protein Family
B-lymphocyte antigen
UniProt Gene Name
CD19
UniProt Entry Name
CD19_HUMAN
NCBI Protein Information
B-lymphocyte antigen CD19

NCBI Summary for CD19

Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq, Jul 2008]
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UniProt Comments for CD19

CD19: a cell surface molecule which assembles with the antigen receptor of B lymphocytes. A critical signal transduction molecule that regulates B lymphocyte development, activation, and differentiation. Ligation increases intracellular calcium levels and decreases the threshold for antigen receptor signaling. Germinal center formation is significantly reduced in CD19-deficient mice.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: external side of plasma membrane; integral to plasma membrane; plasma membrane; protein complex

Molecular Function: phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein binding; receptor signaling protein activity

Biological Process: B cell receptor signaling pathway; cell surface receptor linked signal transduction; cellular defense response; phosphoinositide-mediated signaling; regulation of immune response; regulation of phosphoinositide 3-kinase cascade

Disease: Immunodeficiency, Common Variable, 2; Immunodeficiency, Common Variable, 3
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Research Articles on CD19

1. High anti-EBV IgG levels in Crohn's disease are associated with 5-aminosalicylic acid treatment, tonsillectomy, and decrease of CD19(+) cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD19 antibody

 Reference Product  PubMed Publications
 CD22 antibody  >660 publications with CD19 and CD22
 CD79A antibody  >172 publications with CD19 and CD79A
 CD79B antibody  >120 publications with CD19 and CD79B
 CD81 antibody  >117 publications with CD19 and CD81
 CR2 antibody  >79 publications with CD19 and CR2
 LYN antibody  >65 publications with CD19 and LYN
 SYK antibody  >54 publications with CD19 and SYK
 VAV1 antibody  >15 publications with CD19 and VAV1
 PIK3R1 antibody  >3 publications with CD19 and PIK3R1

Pathways associated with anti-CD19 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Activates B Cell Receptor (BCR) Leading To Generation Of Second Messengers Pathway antibodies  Antigen Activates B Cell Receptor (BCR) Leading To Generation Of Second Messengers Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 BCR Signaling Pathway antibodies  BCR Signaling Pathway Diagram
 Constitutive Signaling By Aberrant PI3K In Cancer Pathway antibodies  Constitutive Signaling By Aberrant PI3K In Cancer Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram

Diseases associated with anti-CD19 antibody

 Disease Name  Pubmed Publications
 Vascular Diseases Antibodies  >458 publications with CD19 and Vascular Diseases
 Inflammation Antibodies  >426 publications with CD19 and Inflammation
 Necrosis Antibodies  >357 publications with CD19 and Necrosis
 Disease Models, Animal Antibodies  >280 publications with CD19 and Disease Models, Animal
 Lung Diseases Antibodies  >228 publications with CD19 and Lung Diseases
 Liver Diseases Antibodies  >183 publications with CD19 and Liver Diseases
 Kidney Diseases Antibodies  >152 publications with CD19 and Kidney Diseases
 Cell Transformation, Neoplastic Antibodies  >142 publications with CD19 and Cell Transformation, Neoplastic
 Common Variable Immunodeficiency Antibodies  >98 publications with CD19 and Common Variable Immunodeficiency
 Agammaglobulinemia Antibodies  >95 publications with CD19 and Agammaglobulinemia

Organs/Tissues associated with anti-CD19 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >6846 publications with CD19 and Blood
 Bone Marrow Antibodies  >1872 publications with CD19 and Bone Marrow
 Spleen Antibodies  >637 publications with CD19 and Spleen
 Lymph Node Antibodies  >510 publications with CD19 and Lymph Node
 Lymph Antibodies  >457 publications with CD19 and Lymph
 Lung Antibodies  >239 publications with CD19 and Lung
 Thymus Antibodies  >182 publications with CD19 and Thymus
 Brain Antibodies  >155 publications with CD19 and Brain
 Tonsil Antibodies  >127 publications with CD19 and Tonsil
 Intestine Antibodies  >113 publications with CD19 and Intestine
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