Mouse Anti-CGA Monoclonal Antibody – Cat# MBS438790

Formalin-fixed, paraffin-embedded human Placenta stained with hCG Monoclonal Antibody (HCGab/52).

HCG Holo (CGA) Antibody – Mouse Monoclonal

Name: CGA Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS438790
Price: 280 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS438790

MBS438790 is an antibody targeting CGA. Also known as HCG Holo (Pregnancy & Choriocarcinoma Marker) Mouse Monoclonal Antibody. Reactive in Human. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

HCG Holo (CGA), Monoclonal Antibody

Full Product Name

HCG Holo (Pregnancy & Choriocarcinoma Marker) Mouse Monoclonal Antibody

Product Gene Name

anti-CGA antibody

Product Synonym Gene Name

CGB

Product Synonym Names

CG-alpha; CGA; Chorionic Gonadotrophin Alpha; Follicle Stimulating Hormone Alpha; Follitropin Alpha; FSH-alpha; FSHA; GPH Alpha; GPHA1; LHA; LH-alpha; Luteinizing Hormone Alpha; Lutropin Alpha; Thyroid Stimulating Hormone Alpha; Thyrotropin Alpha; TSHA; CG-beta; CGB3; CGB5; CGB7; CGB8; Choriogonadotropin Subunit beta; hCGB

Clonality

Monoclonal

Isotype

IgG1, kappa

Clone Number

HCGab/52

Host

Mouse

Species Reactivity

Human

Form/Format

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Specificity

This MAb is a very special because it reacts ONLY with the intact-HCG and not with either free alpha- or free beta-chain of HCG. HCG is a glycoprotein and is composed of two non-identical, non-covalently linked polypeptide chains designated as the alpha and beta subunits. The alpha subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH). HCG is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG MAb detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate anti-hCG positivity in 90% and 60% of cases respectively. 20% of lung squamous cell carcinomas are positive. HCG expression by non-trophoblastic tumors may indicate aggressive behavior.

Preparation and Storage

Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Molecular Weight

~35kDa (intact hCG)

OMIM

118850

3D Structure

ModBase 3D Structure for P01215

Cellular Localization

Cytoplasmic, Secreted

Immunogen

Purified hCG protein

Hu-Chromosome Location

6q12-q21 (HCG-alpha) & 19q13.33 (HCG-beta)

Positive Control

JAR or TT Cells. Placenta

Applications Tested/Suitable for anti-CGA antibody

Immunohistochemistry (IHC) Formalin

Application Notes for anti-CGA antibody

Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Product References and Citations for anti-CGA antibody

McDonald EA et. al. Endocrinology 150:4358-65 (2009).

Other Notes

Small volumes of anti-CGA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CGA. It may not necessarily be applicable to this product.

NCBI GI #

4502787

NCBI GeneID

1081

NCBI Accession #

NP_000726.1

UniProt Primary Accession #

P01215

UniProt Secondary Accession #

P01233

UniProt Related Accession #

P01215

GenBank Nucleotide #

NM_000735.3

NCBI Official Full Name

glycoprotein hormones alpha chain isoform 2

NCBI Official Synonym Full Names

glycoprotein hormones, alpha polypeptide

NCBI Official Symbol

CGA

NCBI Official Synonym Symbols

HCG; LHA; FSHA; GPHa; TSHA; GPHA1; CG-ALPHA

UniProt Protein Name

Glycoprotein hormones alpha chain

UniProt Synonym Protein Names

Anterior pituitary glycoprotein hormones common subunit alpha; Choriogonadotropin alpha chain; Chorionic gonadotrophin subunit alpha; CG-alpha; Follicle-stimulating hormone alpha chain; FSH-alpha; Follitropin alpha chain; Luteinizing hormone alpha chain; LSH-alpha; Lutropin alpha chain; Thyroid-stimulating hormone alpha chain; TSH-alpha; Thyrotropin alpha chain

Protein Family

Glycoprotein hormones

UniProt Gene Name

CGA

UniProt Synonym Gene Names

CG-alpha; FSH-alpha; LSH-alpha; TSH-alpha

UniProt Entry Name

GLHA_HUMAN

NCBI Protein Information

glycoprotein hormones alpha chain

NCBI Summary for CGA

The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. The protein encoded by this gene is the alpha subunit and belongs to the glycoprotein hormones alpha chain family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

UniProt Comments for CGA

CGA: The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. The protein encoded by this gene is the alpha subunit and belongs to the glycoprotein hormones alpha chain family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 6q12-q21

Cellular Component: extracellular region; Golgi lumen

Molecular Function: hormone activity; protein binding

Biological Process: cell-cell signaling; peptide hormone processing; signal transduction

Research Articles on CGA

1. The present results have for the first time shown protective effects exerted by hCG on vascular endothelial function.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CGA antibody

Reference Product	PubMed Publications
LHB antibody	>37 publications with CGA and LHB
FSHB antibody	>34 publications with CGA and FSHB
POMC antibody	>22 publications with CGA and POMC
TSHB antibody	>9 publications with CGA and TSHB
FSHR antibody	>6 publications with CGA and FSHR
LHCGR antibody	>3 publications with CGA and LHCGR
CGB5 antibody	>1 publications with CGA and CGB5
CGB8 antibody	>1 publications with CGA and CGB8

Pathways associated with anti-CGA antibody

Products by Pathway	Pathway Diagram
Amine-derived Hormones Pathway antibodies	Amine-derived Hormones Pathway Diagram
Androgen Biosynthesis Pathway antibodies	Androgen Biosynthesis Pathway Diagram
Asparagine N-linked Glycosylation Pathway antibodies	Asparagine N-linked Glycosylation Pathway Diagram
Autoimmune Thyroid Disease Pathway antibodies	Autoimmune Thyroid Disease Pathway Diagram
Autoimmune Thyroid Disease Pathway antibodies	Autoimmune Thyroid Disease Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
FSH Signaling Pathway antibodies	FSH Signaling Pathway Diagram
G Alpha (s) Signalling Events Pathway antibodies	G Alpha (s) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram

Diseases associated with anti-CGA antibody

Disease Name	Pubmed Publications
Adenocarcinoma Antibodies	>297 publications with CGA and Adenocarcinoma
Nervous System Diseases Antibodies	>193 publications with CGA and Nervous System Diseases
Prostatic Diseases Antibodies	>144 publications with CGA and Prostatic Diseases
Prostatic Neoplasms Antibodies	>143 publications with CGA and Prostatic Neoplasms
Cardiovascular Diseases Antibodies	>124 publications with CGA and Cardiovascular Diseases
Lung Neoplasms Antibodies	>123 publications with CGA and Lung Neoplasms
Disease Progression Antibodies	>116 publications with CGA and Disease Progression
Liver Diseases Antibodies	>82 publications with CGA and Liver Diseases
Inflammation Antibodies	>81 publications with CGA and Inflammation
Hyperplasia Antibodies	>80 publications with CGA and Hyperplasia

Organs/Tissues associated with anti-CGA antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>188 publications with CGA and Brain
Lung Antibodies	>176 publications with CGA and Lung
Liver Antibodies	>163 publications with CGA and Liver
Stomach Antibodies	>145 publications with CGA and Stomach
Thyroid Antibodies	>112 publications with CGA and Thyroid
Vascular Antibodies	>111 publications with CGA and Vascular
Heart Antibodies	>111 publications with CGA and Heart
Muscle Antibodies	>106 publications with CGA and Muscle
Pituitary Gland Antibodies	>82 publications with CGA and Pituitary Gland
Skin Antibodies	>61 publications with CGA and Skin