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Nuclear Antigen (HNA) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS438815

MBS438815 is an antibody targeting Nuclear Antigen (HNA). Also known as Human Nuclear Antigen (HNA) (Human Cell Marker) Mouse Monoclonal Antibody. Reactive in Human, Non-Human Primates.Does not react with Mouse, Rat and Chicken. Host: Mouse. More details

$500 / 0.5 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Nuclear Antigen (HNA), Monoclonal Antibody
Full Product Name
Human Nuclear Antigen (HNA) (Human Cell Marker) Mouse Monoclonal Antibody
Product Synonym Gene Name
HNA
Product Synonym Names
HNA; HuNu
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
235-1
Host
Mouse
Species Reactivity
Human, Non-Human Primates.
Does not react with Mouse, Rat and Chicken
Form/Format
Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.
Purity/Purification
Purified Ab conjugated to PE
Specificity
This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb 235-1 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
Preparation and Storage
Antibody with azide - store at 4 to 8 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
70kDa; 80kDa
Cellular Localization
Nuclei
Immunogen
Nuclei of human myeloid leukemia biopsy cells
Positive Control
All human cells or tissues
Applications Tested/Suitable for anti-Nuclear Antigen (HNA) antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Flow Cytometry (5ul per test per one million cells in 0.1ml or 5ul per 100ul of whole blood)
Immunofluorescence (1:50-1:100 for 30 minutes at RT)

Flow Cytometry (FC/FACS) of anti-Nuclear Antigen (HNA) antibody

Flow Cytometry of Human Nuclear Antigen on MCF-7 Cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled HNA Monoclonal Antibody (235-1).
Mouse Anti-Nuclear Antigen (HNA) Monoclonal Antibody – Cat# MBS438815

Flow Cytometry (FC/FACS) of anti-Nuclear Antigen (HNA) antibody

Flow Cytometry of Human Nuclear Antigen on HeLa Cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled HNA Monoclonal Antibody (235-1).
Mouse Anti-Nuclear Antigen (HNA) Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS438815

Product References and Citations for anti-Nuclear Antigen (HNA) antibody

Rosario Sanchez-Pernaute et. al. Parthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's disease. Brain. 2008; 131(8): 2127-2139.Glaser R, Lu MM, Narula N, Epstein JA (2002) Smooth muscle cells, but not myocytes, of host origin in transplanted human hearts. Circulation Jul 2;106(1):17-9. Parthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's diseaseParthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's diseaseParthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's diseas

Other Notes

Small volumes of anti-Nuclear Antigen (HNA) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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