Mouse Anti-KRT18 Monoclonal Antibody – Cat# MBS438926

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (KRT18/836).

Cytokeratin 18 (KRT18) Antibody – Mouse Monoclonal

Name: KRT18 Antibody; Monoclonal; Mouse; Human/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS438926
Price: 280 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS438926

MBS438926 is an antibody targeting KRT18. Also known as Cytokeratin 18 (KRT18) Mouse Monoclonal Antibody. Reactive in Human, Dog, Cat.Does not react with Rat. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Cytokeratin 18 (KRT18), Monoclonal Antibody

Full Product Name

Cytokeratin 18 (KRT18) Mouse Monoclonal Antibody

Product Gene Name

anti-KRT18 antibody

Product Synonym Names

Cell Proliferation-inducing Gene 46 Protein; CK18; CYK18 Cytokeratin Endo B; K18; Keratin-18; Kerd; KRT18

Clonality

Monoclonal

Isotype

IgG1

Clone Number

KRT18/836

Host

Mouse

Species Reactivity

Human, Dog, Cat.
Does not react with Rat

Form/Format

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Specificity

This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

Preparation and Storage

Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Molecular Weight

45kDa

OMIM

148070

3D Structure

ModBase 3D Structure for P05783

Cellular Localization

Cytoplasmic

Immunogen

Recombinant human KRT18 protein

Hu-Chromosome Location

12q13.13

Positive Control

MCF-7, HeLa, A431 cells or Breast Cancer

Applications Tested/Suitable for anti-KRT18 antibody

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin

Application Notes for anti-KRT18 antibody

Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Product References and Citations for anti-KRT18 antibody

Lauerova, L., et al. 1988. Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule. Hybridoma 5: 495-504.

Other Notes

Small volumes of anti-KRT18 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for KRT18. It may not necessarily be applicable to this product.

NCBI GI #

4557888

NCBI GeneID

3875

NCBI Accession #

NP_000215.1

UniProt Primary Accession #

P05783

UniProt Secondary Accession #

Q53G38; Q5U0N8; Q9BW26

UniProt Related Accession #

P05783

GenBank Nucleotide #

NM_000224.2

NCBI Official Full Name

keratin, type I cytoskeletal 18

NCBI Official Synonym Full Names

keratin 18

NCBI Official Symbol

KRT18

NCBI Official Synonym Symbols

K18; CK-18; CYK18

UniProt Protein Name

Keratin, type I cytoskeletal 18

UniProt Synonym Protein Names

Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18; K18

Protein Family

Keratin

UniProt Gene Name

KRT18

UniProt Synonym Gene Names

CYK18; CK-18; K18

UniProt Entry Name

K1C18_HUMAN

NCBI Protein Information

keratin, type I cytoskeletal 18

NCBI Summary for KRT18

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

UniProt Comments for KRT18

K18: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Keratin 18 and its filament partner keratin 8 are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Defects are a cause of cryptogenic cirrhosis.

Protein type: Cytoskeletal; Nucleolus

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: cytoplasm; intermediate filament; keratin filament; microtubule organizing center

Molecular Function: protein binding

Biological Process: anatomical structure morphogenesis; Golgi to plasma membrane CFTR protein transport; intermediate filament cytoskeleton organization and biogenesis; negative regulation of apoptosis

Disease: Cirrhosis, Familial

Research Articles on KRT18

1. As an apoptosis-related biomarker, serum M30 [M30 cytokeratin-18 peptide, human ] level has a merit to be the most promising test for prediction or differential diagnosis of HELLP syndrome in PE patients.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-KRT18 antibody

Reference Product	PubMed Publications
KRT8 antibody	>63 publications with KRT18 and KRT8
EGR1 antibody	>1 publications with KRT18 and EGR1
TCHP antibody	>1 publications with KRT18 and TCHP
YWHAZ antibody	>1 publications with KRT18 and YWHAZ
CASP6 antibody	>1 publications with KRT18 and CASP6

Pathways associated with anti-KRT18 antibody

Products by Pathway	Pathway Diagram
Caspase Cascade In Apoptosis Pathway antibodies	Caspase Cascade In Apoptosis Pathway Diagram
EGFR1 Signaling Pathway antibodies	EGFR1 Signaling Pathway Diagram
Pathogenic Escherichia Coli Infection Pathway antibodies	Pathogenic Escherichia Coli Infection Pathway Diagram
Pathogenic Escherichia Coli Infection Pathway antibodies	Pathogenic Escherichia Coli Infection Pathway Diagram
Pathogenic Escherichia Coli Infection Pathway antibodies	Pathogenic Escherichia Coli Infection Pathway Diagram

Diseases associated with anti-KRT18 antibody

Disease Name	Pubmed Publications
Liver Diseases Antibodies	>45 publications with KRT18 and Liver Diseases
Fibrosis Antibodies	>20 publications with KRT18 and Fibrosis
Fatty Liver Antibodies	>19 publications with KRT18 and Fatty Liver
Breast Neoplasms Antibodies	>17 publications with KRT18 and Breast Neoplasms
Neoplasm Metastasis Antibodies	>17 publications with KRT18 and Neoplasm Metastasis
Adenocarcinoma Antibodies	>16 publications with KRT18 and Adenocarcinoma
Inflammation Antibodies	>13 publications with KRT18 and Inflammation
Liver Cirrhosis Antibodies	>11 publications with KRT18 and Liver Cirrhosis
Necrosis Antibodies	>11 publications with KRT18 and Necrosis
Disease Models, Animal Antibodies	>10 publications with KRT18 and Disease Models, Animal

Organs/Tissues associated with anti-KRT18 antibody

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>60 publications with KRT18 and Liver
Blood Antibodies	>38 publications with KRT18 and Blood
Prostate Antibodies	>10 publications with KRT18 and Prostate
Bone Antibodies	>9 publications with KRT18 and Bone
Lymph Antibodies	>8 publications with KRT18 and Lymph
Lymph Node Antibodies	>8 publications with KRT18 and Lymph Node
Muscle Antibodies	>7 publications with KRT18 and Muscle
Kidney Antibodies	>7 publications with KRT18 and Kidney
Stomach Antibodies	>7 publications with KRT18 and Stomach
Eye Antibodies	>7 publications with KRT18 and Eye