• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Macrophage & Histiocytoma Marker Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS438935

MBS438935 is an antibody targeting Macrophage & Histiocytoma Marker. Also known as Macrophage & Histiocytoma Marker Mouse Monoclonal Antibody. Reactive in Human.Does not react with Mouse, Rat and Pig. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Macrophage & Histiocytoma Marker, Monoclonal Antibody
Full Product Name
Macrophage & Histiocytoma Marker Mouse Monoclonal Antibody
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
D11
Host
Mouse
Species Reactivity
Human.
Does not react with Mouse, Rat and Pig
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% azide.
Specificity
In Western blotting, it detects an antigen of 125kDa in human liver and 135kDa in tumors of histiocytic origin. Comparative study of this MAb and a standard CD68 MAb showed that their antigens are different. Its antigen in all macrophage types studied is located on the plasma membrane and within cytoplasmic structures including lysosomes. This MAb shows a restricted reactivity to cells of the monocyte/macrophage system. It specifically reacts with blood monocytes and stains resident macrophages in a wide variety of human tissues. This MAb does not stain antigen-presenting cells, e.g., Langerhans cells. Reportedly, its reactivity is restricted to histiocytes and macrophages.
Preparation and Storage
Antibody with azide - store at 2 to 8°C. Non-hazardous. No MSDS required.
Molecular Weight
125kDa -135kDa
Cellular Localization
Cell Surface & Cytoplasmic
Immunogen
Membrane preparation from human hepatocytes
Positive Control
Liver or Histiocytoma
Applications Tested/Suitable for anti-Macrophage & Histiocytoma Marker antibody
Immunohistochemistry (IHC) Formalin
Application Notes for anti-Macrophage & Histiocytoma Marker antibody
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunohistochemistry (IHC) of anti-Macrophage & Histiocytoma Marker antibody

Formalin-fixed, paraffin-embedded human Histiocytoma stained with Macrophage Monoclonal Antibody (D11).
Mouse Anti-Macrophage & Histiocytoma Marker Monoclonal Antibody – Cat# MBS438935

Immunohistochemistry (IHC) of anti-Macrophage & Histiocytoma Marker antibody

Formalin-fixed, paraffin-embedded human Histiocytoma stained with Macrophage Monoclonal Antibody (D11).
Mouse Anti-Macrophage & Histiocytoma Marker Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS438935

SDS-PAGE of anti-Macrophage & Histiocytoma Marker antibody

SDS-PAGE Analysis Purified Macrophage Antibody (D11). Confirmation of Purity and Integrity of Antibody.
Mouse Anti-Macrophage & Histiocytoma Marker Monoclonal Antibody, SDS-PAGE – Cat# MBS438935

Product References and Citations for anti-Macrophage & Histiocytoma Marker antibody

Rudinskaya TD, Poltoranina VS, Baranov VN, Petrovichev NN, Voytenkov BO, Tretyakov LO. D11, a novel monoclonal antibody specific for human mature macrophages and peripheral blood monocytes. Immunol Lett. 1992 Jun;33(1):1-7. Frenkel MA, Tupitsyn NN, Rudinskaia TD, Poltoranina VS, Lebedeva NB, Volkova MA, Kaletin GI. [Use of a new antimacrophage monoclonal antibody D11 in diagnosis of hemoblastosis]. [Article in Russian] Gematol Transfuziol. 1995 Jul-Aug;40(4):13-6. Tupitsyn NN, et al. Reactivity of anti-macrophage monoclonal antibody D11 in human leukemia and malignant lymphoma. Int J Cancer. 1996;68(2):160-3. Petrovichev NN, et al. Antimacrophage monoclonal antibody D11 in the diagnosis of tumors of histiocytic origin. Acta Cytol. 1997;41(2):357-63.

Other Notes

Small volumes of anti-Macrophage & Histiocytoma Marker antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000