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CD54 / ICAM-1 (ICAM1) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS438953

MBS438953 is an antibody targeting ICAM1. Also known as CD54 / ICAM-1 Mouse Monoclonal Antibody. Reactive in Human. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CD54 / ICAM-1 (ICAM1), Monoclonal Antibody
Full Product Name
CD54 / ICAM-1 Mouse Monoclonal Antibody
Product Gene Name
anti-ICAM1 antibody
Product Synonym Names
CD54; Cell surface glycoprotein P3.58; Human rhinovirus receptor; ICAM-1; Intercellular adhesion molecule 1; Ly 47; Major group rhinovirus receptor; MALA2; MyD10; Surface antigen of activated B cells
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
W-CAM-1; same as Wehi-CAM-1 or 1H4
Host
Mouse
Species Reactivity
Human
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
Recognizes an 85-115kDa protein (variation with cell type), identified as intercellular adhesion molecule (ICAM-1) (Workshop IV). It has 7 potential N-linked glycosylation sites. ICAM-1 is a single chain glycoprotein of Ig supergene family, present on unstimulated endothelial cells (EC) and on a variety of other cell types including activated fibroblasts, EC, macrophages, and lymphocytes. ICAM-1 mediates cell adhesion by binding to integrins CD11a/CD18 (leukocyte adhesion molecule, LFA-1) and to CD11b/CD18 (Mac-1). This interaction enhances antigen-specific T-cell activation. ICAM-1 also binds to CD43 and to Plasmodium falciparum infected RBCs. W-CAM-1 MAb blocks aggregation of cell lines mediated by the ICAM-1 and blocks homotypic binding of purified populations of activated T- and B-lymphocytes and also aggregation of mixed T- and B-cell blasts. It inhibits T-cell adhesion to normal human endothelial cells. Activation induced by cell-cell contact (mixed lymphocyte reaction, T-cell mediated B-cell activation) is significantly inhibited. This MAb blocks elements of both effector arms of immune system (cytotoxic cell function and Ig production).
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
85-115kDa
OMIM
147840
3D Structure
ModBase 3D Structure for P05362
Cellular Localization
Cell Surface
Immunogen
Raji Burkitt lymphoma cells
Hu-Chromosome Location
19p13.3-p13.2
Positive Control
Raji cells, MOLT 4, HeLa or SW480 cells. Human tonsil.
Applications Tested/Suitable for anti-ICAM1 antibody
Functional Studies, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
Application Notes for anti-ICAM1 antibody
Functional Studies (Order Ab without BSA & Azide)
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes)

Immunohistochemistry (IHC) of anti-ICAM1 antibody

Formalin-fixed, paraffin-embedded human Melanoma stained with CD54 Monoclonal Antibody (W-CAM-1).
Mouse Anti-ICAM1 Monoclonal Antibody – Cat# MBS438953

Product References and Citations for anti-ICAM1 antibody

Boyd AW et. al. Blood, 1989, 73(7):1896-903. Boyd AW et. al. Proceedings of the National Academy of Sciences, 1988, 85(9):3095 Wawryk et al. J Clin Pathol 44, 497-501 (1991). Fecondo et al., Proc. Nat. Acad. Sci. 88(7), 28792882, (1991)

Other Notes

Small volumes of anti-ICAM1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ICAM1. It may not necessarily be applicable to this product.

NCBI GI #
167466198
NCBI GeneID
3383
NCBI Accession #
NP_000192.2
UniProt Primary Accession #
P05362
UniProt Secondary Accession #
Q5NKV7; Q96B50; B2R6M3
UniProt Related Accession #
P05362
GenBank Nucleotide #
NM_000201.2
NCBI Official Full Name
intercellular adhesion molecule 1
NCBI Official Synonym Full Names
intercellular adhesion molecule 1
NCBI Official Symbol
ICAM1
NCBI Official Synonym Symbols
BB2; CD54; P3.58
UniProt Protein Name
Intercellular adhesion molecule 1
UniProt Synonym Protein Names
Major group rhinovirus receptor; CD_antigen: CD54
Protein Family
Intercellular adhesion molecule
UniProt Gene Name
ICAM1
UniProt Synonym Gene Names
ICAM-1
UniProt Entry Name
ICAM1_HUMAN
NCBI Protein Information
intercellular adhesion molecule 1

NCBI Summary for ICAM1

This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008]

UniProt Comments for ICAM1

ICAM1: a type I membrane protein of the immunoglobulin superfamily. Is a ligand for the leukocyte adhesion LFA-1 protein (Integrin alpha-L/beta-2) and a Rhinovirus receptor. Typically expressed on endothelial cells and cells of the immune system. ICAM1 binds to integrins of type CD11a / CD18, or CD11b / CD18. Its expression is activated by p53 in an NF-kappaB-independent manner. Induced by TNFalpha in a process that involves IKKbeta.

Protein type: Immunoglobulin superfamily; Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: cell surface; extracellular space; focal adhesion; integral to plasma membrane; membrane; plasma membrane

Molecular Function: integrin binding; protein binding; receptor activity; transmembrane receptor activity

Biological Process: adhesion to host; cell adhesion; extracellular matrix organization and biogenesis; heterophilic cell adhesion; leukocyte adhesion; leukocyte migration; membrane to membrane docking; positive regulation of cellular extravasation; regulation of immune response; regulation of leukocyte mediated cytotoxicity; T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell; virion attachment, binding of host cell surface receptor

Disease: Malaria, Susceptibility To
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Research Articles on ICAM1

1. ICAM1 and CD44 could have a compensation effect on maintaining the stemness characteristics of esophageal squamous cell carcinoma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ICAM1 antibody

 Reference Product  PubMed Publications
 TNF antibody  >3666 publications with ICAM1 and TNF
 RELA antibody  >249 publications with ICAM1 and RELA
 VCAM1 antibody  >140 publications with ICAM1 and VCAM1
 ITGAM antibody  >25 publications with ICAM1 and ITGAM
 NFKB1 antibody  >23 publications with ICAM1 and NFKB1
 IRF1 antibody  >18 publications with ICAM1 and IRF1
 ITGB2 antibody  >7 publications with ICAM1 and ITGB2
 ITGAL antibody  >7 publications with ICAM1 and ITGAL
 EZR antibody  >1 publications with ICAM1 and EZR

Pathways associated with anti-ICAM1 antibody

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 AGE-RAGE Signaling Pathway In Diabetic Complications antibodies  AGE-RAGE Signaling Pathway In Diabetic Complications Diagram
 AGE-RAGE Signaling Pathway In Diabetic Complications antibodies  AGE-RAGE Signaling Pathway In Diabetic Complications Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram

Diseases associated with anti-ICAM1 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >6149 publications with ICAM1 and Inflammation
 Necrosis Antibodies  >5093 publications with ICAM1 and Necrosis
 Cardiovascular Diseases Antibodies  >3949 publications with ICAM1 and Cardiovascular Diseases
 Atherosclerosis Antibodies  >1602 publications with ICAM1 and Atherosclerosis
 Heart Diseases Antibodies  >1094 publications with ICAM1 and Heart Diseases
 Reperfusion Injury Antibodies  >937 publications with ICAM1 and Reperfusion Injury
 Myocardial Ischemia Antibodies  >842 publications with ICAM1 and Myocardial Ischemia
 Kidney Diseases Antibodies  >825 publications with ICAM1 and Kidney Diseases
 Hypertension Antibodies  >579 publications with ICAM1 and Hypertension
 Asthma Antibodies  >517 publications with ICAM1 and Asthma

Organs/Tissues associated with anti-ICAM1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >12387 publications with ICAM1 and Blood
 Vascular Antibodies  >9358 publications with ICAM1 and Vascular
 Lung Antibodies  >1900 publications with ICAM1 and Lung
 Heart Antibodies  >1399 publications with ICAM1 and Heart
 Brain Antibodies  >1273 publications with ICAM1 and Brain
 Kidney Antibodies  >1253 publications with ICAM1 and Kidney
 Liver Antibodies  >1237 publications with ICAM1 and Liver
 Skin Antibodies  >1233 publications with ICAM1 and Skin
 Muscle Antibodies  >1075 publications with ICAM1 and Muscle
 Embryonic Tissue Antibodies  >793 publications with ICAM1 and Embryonic Tissue
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