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Neurofilament (Nf-L) (NEFL) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS439019

MBS439019 is an antibody targeting NEFL. Also known as Neurofilament (NF-L) (Neuronal Marker) Mouse Monoclonal Antibody. Reactive in Human, Rat, Pig, Cow, Chicken. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Neurofilament (NF-L) (NEFL), Monoclonal Antibody
Full Product Name
Neurofilament (NF-L) (Neuronal Marker) Mouse Monoclonal Antibody
Product Gene Name
anti-NEFL antibody
Product Synonym Names
68kDa neurofilament protein, Light molecular weight neurofilament protein, NEFL, Neurofilament light polypeptide 68kDa, Neurofilament light polypeptide, Neurofilament protein, light chain, Neurofilament subunit NF-L, Neurofilament triplet L protein, NF-L, NF68
Clonality
Monoclonal
Isotype
IgG1
Clone Number
NFL/736
Host
Mouse
Species Reactivity
Human, Rat, Pig, Cow, Chicken
Form/Format
1.0mg/ml of Ab purified from Bioreactor Concentrate by Protein A/G.
Specificity
This MAb reacts with a 68kDa protein, identified as light sub-unit of neurofilaments (NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.
Preparation and Storage
Antibody without azide - store at -20°C to -80°C. Non-hazardous. No MSDS required.
Molecular Weight
68kDa
OMIM
162280
3D Structure
ModBase 3D Structure for P07196
Cellular Localization
Cytoplasmic
Immunogen
Recombinant human NEFL protein
Hu-Chromosome Location
8p21.2
Positive Control
HEK293 cells; Brain, Neuroblastoma.
Applications Tested/Suitable for anti-NEFL antibody
Immunofluorescence (IF), Flow Cytometry (FC), Immunohistochemistry (IHC) Formalin
Application Notes for anti-NEFL antibody
Immunofluorescence (1-2ug/ml)
Flow Cytometry (1-2ug/million cells)
Immunohistochemistry (Formalin-fixed)
(0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunohistochemistry (IHC) of anti-NEFL antibody

Formalin-fixed, paraffin-embedded Rat Cerebellum stained with Neurofilament Monoclonal Antibody (NFL/736).
Mouse Anti-NEFL Monoclonal Antibody – Cat# MBS439019

Flow Cytometry (FC) of anti-NEFL antibody

Flow Cytometric Analysis of HEK293 cells using Neurofilament Monoclonal Antibody (NFL/736) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
Mouse Anti-NEFL Monoclonal Antibody, Flow Cytometry (FC) – Cat# MBS439019

Product References and Citations for anti-NEFL antibody

Angelides, K.J., et. al. 1989. J. Cell Biol. 108: 1495-1506.

Other Notes

Small volumes of anti-NEFL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NEFL. It may not necessarily be applicable to this product.

NCBI GI #
105990539
NCBI GeneID
4747
NCBI Accession #
NP_006149.2
UniProt Primary Accession #
P07196
UniProt Secondary Accession #
Q16154; Q8IU72; B9ZVN2
UniProt Related Accession #
P07196
GenBank Nucleotide #
NM_006158.4
NCBI Official Full Name
neurofilament light polypeptide
NCBI Official Synonym Full Names
neurofilament, light polypeptide
NCBI Official Symbol
NEFL
NCBI Official Synonym Symbols
NFL; NF-L; NF68; CMT1F; CMT2E; PPP1R110
UniProt Protein Name
Neurofilament light polypeptide
UniProt Synonym Protein Names
68 kDa neurofilament protein; Neurofilament triplet L protein
Protein Family
Neurofilament light polypeptide
UniProt Gene Name
NEFL
UniProt Synonym Gene Names
NF68; NFL; NF-L
UniProt Entry Name
NFL_HUMAN
NCBI Protein Information
neurofilament light polypeptide

NCBI Summary for NEFL

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]
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UniProt Comments for NEFL

NFL: one of the three (L, M, and H) intermediate filament proteins that form neurofilaments. Neurofilaments are involved in the maintenance of neuronal caliber. NF-L is the most abundant of the three neurofilament proteins. Defects cause Charcot-Marie-Tooth disease type 1F (CMT1F).

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 8p21

Cellular Component: axon; cytoplasm; cytosol; neurofilament; TSC1-TSC2 complex

Molecular Function: identical protein binding; protein binding; protein binding, bridging; protein C-terminus binding; Ras guanyl-nucleotide exchange factor activity; structural constituent of cytoskeleton

Biological Process: anterograde axon cargo transport; axon transport of mitochondrion; intermediate filament organization; MAPKKK cascade; neurofilament bundle assembly; retrograde axon cargo transport

Disease: Charcot-marie-tooth Disease, Axonal, Type 2e; Charcot-marie-tooth Disease, Demyelinating, Type 1f
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Research Articles on NEFL

1. Finally, we demonstrated that NEFL inhibited the NF-kappaB pathway, thereby suppressing the expression of uPA and decreasing NSCLC invasiveness and migration.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-NEFL antibody

 Products by Pathway  Pathway Diagram
 ARMS-mediated Activation Pathway antibodies  ARMS-mediated Activation Pathway Diagram
 Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway antibodies  Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 CREB Phosphorylation Through The Activation Of CaMKII Pathway antibodies  CREB Phosphorylation Through The Activation Of CaMKII Pathway Diagram
 CREB Phosphorylation Through The Activation Of Ras Pathway antibodies  CREB Phosphorylation Through The Activation Of Ras Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram

Diseases associated with anti-NEFL antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >69 publications with NEFL and Nervous System Diseases
 Peripheral Nervous System Diseases Antibodies  >56 publications with NEFL and Peripheral Nervous System Diseases
 Atrophy Antibodies  >12 publications with NEFL and Atrophy
 Brain Diseases Antibodies  >9 publications with NEFL and Brain Diseases
 Breast Neoplasms Antibodies  >9 publications with NEFL and Breast Neoplasms
 Disease Models, Animal Antibodies  >6 publications with NEFL and Disease Models, Animal
 Charcot-Marie-Tooth disease, Type 2E Antibodies  >4 publications with NEFL and Charcot-Marie-Tooth disease, Type 2E
 Ataxia Antibodies  >3 publications with NEFL and Ataxia
 Lung Neoplasms Antibodies  >3 publications with NEFL and Lung Neoplasms
 Hypertension Antibodies  >2 publications with NEFL and Hypertension

Organs/Tissues associated with anti-NEFL antibody

 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >42 publications with NEFL and Nerve
 Brain Antibodies  >32 publications with NEFL and Brain
 Prostate Antibodies  >11 publications with NEFL and Prostate
 Spinal Cord Antibodies  >9 publications with NEFL and Spinal Cord
 Lung Antibodies  >6 publications with NEFL and Lung
 Eye Antibodies  >3 publications with NEFL and Eye
 Ganglia Antibodies  >2 publications with NEFL and Ganglia
 Embryonic Tissue Antibodies  >2 publications with NEFL and Embryonic Tissue
 Bone Marrow Antibodies  >2 publications with NEFL and Bone Marrow
 Kidney Antibodies  >2 publications with NEFL and Kidney
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