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AMP Deaminase, Isoform E (AMPD3) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS439031

MBS439031 is an antibody targeting AMPD3. Also known as AMP Deaminase, Isoform E (AMPD3) (Erythroid Marker) Mouse Monoclonal Antibody. Reactive in Human. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
AMP Deaminase, Isoform E (AMPD3), Monoclonal Antibody
Full Product Name
AMP Deaminase, Isoform E (AMPD3) (Erythroid Marker) Mouse Monoclonal Antibody
Product Gene Name
anti-AMPD3 antibody
Product Synonym Names
Adenosine monophosphate deaminase (isoform E); AMP aminohydrolase; AMP deaminase 3; AMP deaminase isoform E; Ampd3; Erythrocyte AMP deaminase; Erythrocyte specific AMP deaminase; Erythrocyte specific protein; Erythrocyte type AMP deaminase; Myoadenylate deaminase
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
AMPD3/901
Host
Mouse
Species Reactivity
Human
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
It recognizes a protein of ~90kDa, which is identified as Adenosine Monophosphate Deaminase, isoform E (AMPD3). It has 767 amino acids and is assigned an EC 3.5.4.6. It is a highly regulated enzyme that catalyzes the hydrolytic deamination of adenosine monophosphate to inosine monophosphate, a branch point in the adenylate catabolic pathway. AMPD3 gene encodes the erythrocyte (E) isoforms, whereas other family members encode isoforms that predominate in muscle (M) and liver (L) cells. This MAb shows reactivity with cells of the erythroid lineage at all stages of maturation in the peripheral blood, bone marrow, and fetal liver. Non-erythroid lineages are negative by flow cytometry. This MAb is useful in the erythroleukemia, identification of bone marrow erythroid precursors, gating erythroid nucleated precursor cells from malignant cells in bone marrow specimens.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
~90kDa
OMIM
102772
3D Structure
ModBase 3D Structure for Q01432
Cellular Localization
Cell Surface
Immunogen
Recombinant full-length human AMDP3 protein
Hu-Chromosome Location
11p15.4
Positive Control
RBC. Fetal liver; Spleen or Placenta
Applications Tested/Suitable for anti-AMPD3 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin
Application Notes for anti-AMPD3 antibody
Flow Cytometry (1-2ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Immunocytochemistry (1-2.0ug/ml for 30 minutes at RT)
Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Immunohistochemistry (IHC) of anti-AMPD3 antibody

Formalin-fixed, paraffin-embedded human Tonsil stained with AMPD3 Monoclonal Antibody (AMPD3/901)
Mouse Anti-AMPD3 Monoclonal Antibody – Cat# MBS439031

Immunohistochemistry (IHC) of anti-AMPD3 antibody

Formalin-fixed, paraffin-embedded human Placenta stained with AMPD3 Monoclonal Antibody (AMPD3/901)
Mouse Anti-AMPD3 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS439031

Product References and Citations for anti-AMPD3 antibody

Sabina RL, Waldenstrm A, Ronquist G. The contribution of Ca+ calmodulin activation of human erythrocyte AMP deaminase (isoform E) to the erythrocyte metabolic dysregulation of familial phosphofructokinase deficiency. Haematologica. 2006;91(5):652-5

Other Notes

Small volumes of anti-AMPD3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for AMPD3. It may not necessarily be applicable to this product.

NCBI GI #
4502079
NCBI GeneID
272
NCBI Accession #
NP_000471.1
UniProt Primary Accession #
Q01432
UniProt Secondary Accession #
A0AUX0; B7Z2S2; B7Z763; B7Z877
UniProt Related Accession #
Q01432
GenBank Nucleotide #
NM_000480.2
NCBI Official Full Name
AMP deaminase 3 isoform 1A
NCBI Official Synonym Full Names
adenosine monophosphate deaminase 3
NCBI Official Symbol
AMPD3
UniProt Protein Name
AMP deaminase 3
UniProt Synonym Protein Names
AMP deaminase isoform E; Erythrocyte AMP deaminase
Protein Family
AMP deaminase
UniProt Gene Name
AMPD3
UniProt Entry Name
AMPD3_HUMAN
NCBI Protein Information
AMP deaminase 3

NCBI Summary for AMPD3

This gene encodes a member of the AMP deaminase gene family. The encoded protein is a highly regulated enzyme that catalyzes the hydrolytic deamination of adenosine monophosphate to inosine monophosphate, a branch point in the adenylate catabolic pathway. This gene encodes the erythrocyte (E) isoforms, whereas other family members encode isoforms that predominate in muscle (M) and liver (L) cells. Mutations in this gene lead to the clinically asymptomatic, autosomal recessive condition erythrocyte AMP deaminase deficiency. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008]
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UniProt Comments for AMPD3

AMP deaminase plays a critical role in energy metabolism.

Research Articles on AMPD3

1. Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-AMPD3 antibody

 Products by Pathway  Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Nucleotides Pathway antibodies  Metabolism Of Nucleotides Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Salvage Pathway antibodies  Purine Salvage Pathway Diagram

Diseases associated with anti-AMPD3 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >4 publications with AMPD3 and Inflammation
 Lung Injury Antibodies  >3 publications with AMPD3 and Lung Injury
 Carcinoma, Hepatocellular Antibodies  >1 publications with AMPD3 and Carcinoma, Hepatocellular
 Hemorrhage Antibodies  >1 publications with AMPD3 and Hemorrhage
 Liver Neoplasms Antibodies  >1 publications with AMPD3 and Liver Neoplasms
 Lung Neoplasms Antibodies  >1 publications with AMPD3 and Lung Neoplasms
 Neoplasms, Experimental Antibodies  >1 publications with AMPD3 and Neoplasms, Experimental
 Liver Diseases Antibodies  >1 publications with AMPD3 and Liver Diseases
 Fibrosis Antibodies  >1 publications with AMPD3 and Fibrosis
 Necrosis Antibodies  >1 publications with AMPD3 and Necrosis

Organs/Tissues associated with anti-AMPD3 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >17 publications with AMPD3 and Muscle
 Blood Antibodies  >10 publications with AMPD3 and Blood
 Liver Antibodies  >7 publications with AMPD3 and Liver
 Heart Antibodies  >5 publications with AMPD3 and Heart
 Lung Antibodies  >5 publications with AMPD3 and Lung
 Placenta Antibodies  >3 publications with AMPD3 and Placenta
 Embryonic Tissue Antibodies  >2 publications with AMPD3 and Embryonic Tissue
 Brain Antibodies  >2 publications with AMPD3 and Brain
 Kidney Antibodies  >1 publications with AMPD3 and Kidney
 Eye Antibodies  >1 publications with AMPD3 and Eye
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