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Napsin A (NAPSA) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS439056

MBS439056 is an antibody targeting NAPSA. Also known as Napsin A (Lung Adenocarcinoma Marker) Mouse Monoclonal Antibody. Reactive in Human. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Napsin A (NAPSA), Monoclonal Antibody
Full Product Name
Napsin A (Lung Adenocarcinoma Marker) Mouse Monoclonal Antibody
Product Gene Name
anti-NAPSA antibody
Product Synonym Names
ASP4, Aspartyl protease 4, KAP, Kidney derived aspartic protease like protein (Kdap), NAP1, NAPA, Napsa, napsin A aspartic peptidase, Pronapsin A, SNAPA
Clonality
Monoclonal
Isotype
IgG, kappa
Clone Number
NAPSA/1238
Host
Mouse
Species Reactivity
Human
Form/Format
200ug/ml of Ab purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Specificity
Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B. There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
37kDa
OMIM
605631
3D Structure
ModBase 3D Structure for O96009
Cellular Localization
Cytoplasmic
Immunogen
Recombinant fragment of human Napsin-A protein
Hu-Chromosome Location
19q13.33
Positive Control
Lung adenocarcinoma
Applications Tested/Suitable for anti-NAPSA antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
Application Notes for anti-NAPSA antibody
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Western Blot (WB) of anti-NAPSA antibody

Western Blot of K562 and HEK293 Cell Lysates using Napsin-A Monoclonal Antibody (NAPSA/1238)
Mouse Anti-NAPSA Monoclonal Antibody – Cat# MBS439056

Immunohistochemistry (IHC) of anti-NAPSA antibody

Formalin-fixed, paraffin-embedded human Lung Adenocarcinoma stained with Napsin-A Monoclonal Antibody (NAPSA/1238).
Mouse Anti-NAPSA Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS439056

Product References and Citations for anti-NAPSA antibody

Bishop, JA et. al. Hum Pathol 41: 20-25 Ordonez, NG 2012 Adv Anat Pathol 19: 66-73 Ye, J et. al. Appl Immunhistochem Mol Morphol 19: 313-31

Other Notes

Small volumes of anti-NAPSA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NAPSA. It may not necessarily be applicable to this product.

NCBI GI #
4758754
NCBI GeneID
9476
NCBI Accession #
NP_004842.1
UniProt Primary Accession #
O96009
UniProt Secondary Accession #
Q8WWD9
UniProt Related Accession #
O96009
GenBank Nucleotide #
NM_004851.2
NCBI Official Full Name
napsin-A preproprotein
NCBI Official Synonym Full Names
napsin A aspartic peptidase
NCBI Official Symbol
NAPSA
NCBI Official Synonym Symbols
KAP; Kdap; NAP1; NAPA; SNAPA
UniProt Protein Name
Napsin-A
UniProt Synonym Protein Names
Aspartyl protease 4; ASP4; Asp 4; Napsin-1; TA01/TA02
Protein Family
Napsin
UniProt Gene Name
NAPSA
UniProt Synonym Gene Names
NAP1; NAPA; ASP4; Asp 4
UniProt Entry Name
NAPSA_HUMAN
NCBI Protein Information
napsin-A

NCBI Summary for NAPSA

This gene encodes a member of the peptidase A1 family of aspartic proteases. The encoded preproprotein is proteolytically processed to generate an activation peptide and the mature protease. The activation peptides of aspartic proteinases function as inhibitors of the protease active site. These peptide segments, or pro-parts, are deemed important for correct folding, targeting, and control of the activation of aspartic proteinase zymogens. The encoded protease may play a role in the proteolytic processing of pulmonary surfactant protein B in the lung and may function in protein catabolism in the renal proximal tubules. This gene has been described as a marker for lung adenocarcinoma and renal cell carcinoma. [provided by RefSeq, Feb 2016]
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UniProt Comments for NAPSA

NAPSA: May be involved in processing of pneumocyte surfactant precursors. Belongs to the peptidase A1 family.

Protein type: Secreted, signal peptide; EC 3.4.23.-; Secreted; Protease

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: extracellular space; lysosome

Molecular Function: aspartic-type endopeptidase activity; endopeptidase activity; peptidase activity

Biological Process: cellular protein metabolic process; membrane protein proteolysis; protein catabolic process; surfactant homeostasis
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Research Articles on NAPSA

1. Combining HNF-1beta and napsin A may distinguish clear cell carcinoma from high-grade serous carcinoma, endometrioid adenocarcinoma and metastatic Krukenberg tumors.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-NAPSA antibody

 Products by Pathway  Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Surfactant Metabolism Pathway antibodies  Surfactant Metabolism Pathway Diagram

Diseases associated with anti-NAPSA antibody

 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >9 publications with NAPSA and Kidney Diseases
 Liver Neoplasms Antibodies  >4 publications with NAPSA and Liver Neoplasms
 Fibrosis Antibodies  >3 publications with NAPSA and Fibrosis
 Neoplasms, Experimental Antibodies  >3 publications with NAPSA and Neoplasms, Experimental
 Carcinoma, Hepatocellular Antibodies  >2 publications with NAPSA and Carcinoma, Hepatocellular
 Liver Diseases Antibodies  >2 publications with NAPSA and Liver Diseases
 Cardiovascular Diseases Antibodies  >1 publications with NAPSA and Cardiovascular Diseases
 Hyperplasia Antibodies  >1 publications with NAPSA and Hyperplasia
 Disease Models, Animal Antibodies  >1 publications with NAPSA and Disease Models, Animal
 Inflammation Antibodies  >1 publications with NAPSA and Inflammation

Organs/Tissues associated with anti-NAPSA antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >82 publications with NAPSA and Lung
 Kidney Antibodies  >18 publications with NAPSA and Kidney
 Uterus Antibodies  >3 publications with NAPSA and Uterus
 Prostate Antibodies  >3 publications with NAPSA and Prostate
 Spleen Antibodies  >2 publications with NAPSA and Spleen
 Trachea Antibodies  >1 publications with NAPSA and Trachea
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