Mouse Anti-VCAM1 Monoclonal Antibody – Cat# MBS439086

CD106 / VCAM1 (VCAM1) Antibody – Mouse Monoclonal

Name: VCAM1 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS439086
Price: 280 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS439086

MBS439086 is an antibody targeting VCAM1. Also known as CD106 / VCAM1 (Activated Endothelial Cell Marker) Mouse Monoclonal Antibody. Reactive in Human. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CD106 / VCAM1, Monoclonal Antibody

Full Product Name

CD106 / VCAM1 (Activated Endothelial Cell Marker) Mouse Monoclonal Antibody

Product Gene Name

anti-VCAM1 antibody

Product Synonym Names

CD106; INCAM-100; L1CAM; Vascular Cell Adhesion Molecule 1 (VCAM1); Vascular cell adhesion protein 1

Clonality

Monoclonal

Isotype

IgG1, kappa

Clone Number

B-K9

Host

Mouse

Species Reactivity

Human

Form/Format

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Specificity

Recognizes a protein of 110kDa, identified as CD106 (also known as vascular cell adhesion molecule-1 (VCAM-1) and INCAM-100). CD106 is a member of the Ig superfamily of adhesion molecules and is expressed at high levels on cytokine stimulated vascular endothelial cells, and at minimal levels on un-stimulated endothelial cells. It is also present on follicular and inter-follicular dendritic cells of lymph nodes, myoblasts, and some macrophages. CD106 serves as a ligand for leukocyte integrin (VLA-4 or CD49d/CD29) and mediates cell adhesion of leukocytes to activated endothelium. It plays a role in various immunological and inflammatory responses. This MAb inhibits the binding of leukocytes to VCAM-1 on stimulated endothelial cells.

Preparation and Storage

Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Molecular Weight

110kDa

OMIM

192225

3D Structure

ModBase 3D Structure for P19320

Cellular Localization

Cell Surface

Immunogen

Activated human umbilical vein endothelial cells (HUVEC)

Hu-Chromosome Location

1q21.2

Positive Control

Human placenta or tonsil

Applications Tested/Suitable for anti-VCAM1 antibody

Flow Cytometry (FC/FACS), Immunofluorescence (IF)

Application Notes for anti-VCAM1 antibody

Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)

Product References and Citations for anti-VCAM1 antibody

Schlossman SF et al. eds. Leukocyte Typing V, p1764-1767, Oxford University Press, Oxford, 1995. Vermot-Desroches C et al. Heterogeneity of antigen expression among human umbilical cord vascular endothelial cells: identification of cell subsets by co-expression of haemopoietic antigens. Immunol Lett 1995, 48(1):1-9 Rice GE et al. Vascular and nonvascular expression of INCAM-110. A target for mononuclear leukocyte adhesion in normal and inflamed human tissues. Am J Pathol 1991, 138(2):385-393 Huang MJ et al. Expression of vascular cell adhesion molecule-1 by follicular dendritic cells. Leuk Lymphoma 1995, 18(3-4):259-264. Rosen GD et al. Roles for the integrin VLA-4 and its counter receptor VCAM-1 in myogenesis. Cell 1992, 69(7):1107-1119 Bevilacqua MP. Endothelial-leukocyte adhesion molecules. Annu Rev Immunol 1993;11:767-804 Foster CA. VCAM-1/alpha 4-integrin adhesion pathway: therapeutic target for allergic inflammatory disorders. J Allergy Clin Immunol 1996, 98(6 Pt 2):S270-277

Other Notes

Small volumes of anti-VCAM1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for VCAM1. It may not necessarily be applicable to this product.

NCBI GI #

4507875

NCBI GeneID

7412

NCBI Accession #

NP_001069.1

UniProt Primary Accession #

P19320

UniProt Secondary Accession #

Q6NUP8; A8K6R7; B4DKS4; E9PDD1

UniProt Related Accession #

P19320

GenBank Nucleotide #

NM_001078.3

NCBI Official Full Name

vascular cell adhesion protein 1 isoform a

NCBI Official Synonym Full Names

vascular cell adhesion molecule 1

NCBI Official Symbol

VCAM1

NCBI Official Synonym Symbols

CD106; INCAM-100

UniProt Protein Name

Vascular cell adhesion protein 1

UniProt Synonym Protein Names

INCAM-100; CD_antigen: CD106

Protein Family

Vascular cell adhesion protein

UniProt Gene Name

VCAM1

UniProt Synonym Gene Names

L1CAM; V-CAM 1; VCAM-1

UniProt Entry Name

VCAM1_HUMAN

NCBI Protein Information

vascular cell adhesion protein 1

NCBI Summary for VCAM1

This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2010]

UniProt Comments for VCAM1

VCAM1: Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion

Chromosomal Location of Human Ortholog: 1p32-p31

Cellular Component: apical part of cell; cell surface; early endosome; endoplasmic reticulum; external side of plasma membrane; extracellular space; filopodium; Golgi apparatus; microvillus; plasma membrane; podosome

Molecular Function: amine oxidase activity; cell adhesion molecule binding; integrin binding

Biological Process: amine metabolic process; B cell differentiation; cell adhesion; cell-matrix adhesion; extracellular matrix organization and biogenesis; heterophilic cell adhesion; leukocyte adhesion; leukocyte tethering or rolling; membrane to membrane docking; positive regulation of T cell proliferation; regulation of immune response

Research Articles on VCAM1

1. Identify the selectins ICAM-1 and VCAM-1 as strong and independent predictors of all-cause and cardiovascular mortality in patients with stable carotid atherosclerosis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-VCAM1 antibody

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African Trypanosomiasis Pathway antibodies	African Trypanosomiasis Pathway Diagram
African Trypanosomiasis Pathway antibodies	African Trypanosomiasis Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cellular Roles Of Anthrax Toxin Pathway antibodies	Cellular Roles Of Anthrax Toxin Pathway Diagram
Cytokine Signaling In Immune System Pathway antibodies	Cytokine Signaling In Immune System Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram

Diseases associated with anti-VCAM1 antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>122 publications with VCAM1 and Inflammation
Cardiovascular Diseases Antibodies	>79 publications with VCAM1 and Cardiovascular Diseases
Necrosis Antibodies	>62 publications with VCAM1 and Necrosis
Atherosclerosis Antibodies	>58 publications with VCAM1 and Atherosclerosis
Heart Diseases Antibodies	>21 publications with VCAM1 and Heart Diseases
Kidney Diseases Antibodies	>20 publications with VCAM1 and Kidney Diseases
Fibrosis Antibodies	>16 publications with VCAM1 and Fibrosis
Hypertension Antibodies	>14 publications with VCAM1 and Hypertension
Lung Diseases Antibodies	>13 publications with VCAM1 and Lung Diseases
Myocardial Ischemia Antibodies	>10 publications with VCAM1 and Myocardial Ischemia

Organs/Tissues associated with anti-VCAM1 antibody

Organ/Tissue Name	Pubmed Publications
Vascular Antibodies	>259 publications with VCAM1 and Vascular
Blood Antibodies	>154 publications with VCAM1 and Blood
Heart Antibodies	>33 publications with VCAM1 and Heart
Brain Antibodies	>30 publications with VCAM1 and Brain
Kidney Antibodies	>28 publications with VCAM1 and Kidney
Embryonic Tissue Antibodies	>25 publications with VCAM1 and Embryonic Tissue
Lung Antibodies	>22 publications with VCAM1 and Lung
Liver Antibodies	>22 publications with VCAM1 and Liver
Skin Antibodies	>10 publications with VCAM1 and Skin
Lymph Node Antibodies	>8 publications with VCAM1 and Lymph Node